Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300

Autores
Passone, Maria Alejandra; Rosso, Laura Cristina; Varela, María Celeste; Ciancio, A.; Etcheverry, Miriam Graciela
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the work was to examine the effects of sub-lethal doses of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) (1+1 mmol/l and 5+5 mmol/l), water activities (0.98, 0.95, 0.93, 0.89 aW) and temperatures (28, 20 °C) on growth, sclerotial characteristics, aflatoxin accumulation and aflD (=nor-1) transcript level by Aspergillus parasiticus RCP08300 on peanut based medium. Growth rate and aflatoxin production were inhibited by BHA-BHT mixture (1+1 mmol/l), regardless of environmental factor assayed. Although sclerotia number and aflD expression were stimulated by this treatment, sclerotia dry weight and volume were reduced by 62.3 and 31.2%, respectively. In contrast, when the fungus grew in presence of the higher dose of BHA-BHT mixture none or very low aflatoxin accumulation and aflD expression occurred. Similarly, A. parasiticus growth has been highly influenced by BHA-BHT (5+5 mmol/l) and interacting stress factors. Data show that sub-lethal antioxidant doses significantly reduced growth and aflatoxin accumulation by A. parasiticus but these treatments were not able to repress the expression of the early expression gene (aflD) involved in aflatoxin biosynthesis. Thus, this represents a high potential risk of stored peanuts contamination with aflatoxins.
Fil: Passone, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Rosso, Laura Cristina. Consiglio Nazionale Delle Ricerche. Istituto Per la Protezione Sostenibile Delle Piante; Italia
Fil: Varela, María Celeste. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ciancio, A.. Consiglio Nazionale Delle Ricerche. Istituto Per la Protezione Sostenibile Delle Piante; Italia
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Materia
AFLATOXINS
AFLD GENE
ANTIOXIDANTS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIAL CHARACTERISTICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192900
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192900
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300Passone, Maria AlejandraRosso, Laura CristinaVarela, María CelesteCiancio, A.Etcheverry, Miriam GracielaAFLATOXINSAFLD GENEANTIOXIDANTSENVIRONMENTAL FACTORSGROWTHSCLEROTIAL CHARACTERISTICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the work was to examine the effects of sub-lethal doses of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) (1+1 mmol/l and 5+5 mmol/l), water activities (0.98, 0.95, 0.93, 0.89 aW) and temperatures (28, 20 °C) on growth, sclerotial characteristics, aflatoxin accumulation and aflD (=nor-1) transcript level by Aspergillus parasiticus RCP08300 on peanut based medium. Growth rate and aflatoxin production were inhibited by BHA-BHT mixture (1+1 mmol/l), regardless of environmental factor assayed. Although sclerotia number and aflD expression were stimulated by this treatment, sclerotia dry weight and volume were reduced by 62.3 and 31.2%, respectively. In contrast, when the fungus grew in presence of the higher dose of BHA-BHT mixture none or very low aflatoxin accumulation and aflD expression occurred. Similarly, A. parasiticus growth has been highly influenced by BHA-BHT (5+5 mmol/l) and interacting stress factors. Data show that sub-lethal antioxidant doses significantly reduced growth and aflatoxin accumulation by A. parasiticus but these treatments were not able to repress the expression of the early expression gene (aflD) involved in aflatoxin biosynthesis. Thus, this represents a high potential risk of stored peanuts contamination with aflatoxins.Fil: Passone, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Rosso, Laura Cristina. Consiglio Nazionale Delle Ricerche. Istituto Per la Protezione Sostenibile Delle Piante; ItaliaFil: Varela, María Celeste. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Ciancio, A.. Consiglio Nazionale Delle Ricerche. Istituto Per la Protezione Sostenibile Delle Piante; ItaliaFil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaWageningen Academic Publishers2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192900Passone, Maria Alejandra; Rosso, Laura Cristina; Varela, María Celeste; Ciancio, A.; Etcheverry, Miriam Graciela; Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300; Wageningen Academic Publishers; World Mycotoxin Journal; 4; 2; 6-2011; 157-1671875-07101875-0796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2010.1261info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.3920/WMJ2010.1261info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:21Zoai:ri.conicet.gov.ar:11336/192900instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:21.99CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
title Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
spellingShingle Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
Passone, Maria Alejandra
AFLATOXINS
AFLD GENE
ANTIOXIDANTS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIAL CHARACTERISTICS
title_short Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
title_full Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
title_fullStr Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
title_full_unstemmed Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
title_sort Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300
dc.creator.none.fl_str_mv Passone, Maria Alejandra
Rosso, Laura Cristina
Varela, María Celeste
Ciancio, A.
Etcheverry, Miriam Graciela
author Passone, Maria Alejandra
author_facet Passone, Maria Alejandra
Rosso, Laura Cristina
Varela, María Celeste
Ciancio, A.
Etcheverry, Miriam Graciela
author_role author
author2 Rosso, Laura Cristina
Varela, María Celeste
Ciancio, A.
Etcheverry, Miriam Graciela
author2_role author
author
author
author
dc.subject.none.fl_str_mv AFLATOXINS
AFLD GENE
ANTIOXIDANTS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIAL CHARACTERISTICS
topic AFLATOXINS
AFLD GENE
ANTIOXIDANTS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIAL CHARACTERISTICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the work was to examine the effects of sub-lethal doses of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) (1+1 mmol/l and 5+5 mmol/l), water activities (0.98, 0.95, 0.93, 0.89 aW) and temperatures (28, 20 °C) on growth, sclerotial characteristics, aflatoxin accumulation and aflD (=nor-1) transcript level by Aspergillus parasiticus RCP08300 on peanut based medium. Growth rate and aflatoxin production were inhibited by BHA-BHT mixture (1+1 mmol/l), regardless of environmental factor assayed. Although sclerotia number and aflD expression were stimulated by this treatment, sclerotia dry weight and volume were reduced by 62.3 and 31.2%, respectively. In contrast, when the fungus grew in presence of the higher dose of BHA-BHT mixture none or very low aflatoxin accumulation and aflD expression occurred. Similarly, A. parasiticus growth has been highly influenced by BHA-BHT (5+5 mmol/l) and interacting stress factors. Data show that sub-lethal antioxidant doses significantly reduced growth and aflatoxin accumulation by A. parasiticus but these treatments were not able to repress the expression of the early expression gene (aflD) involved in aflatoxin biosynthesis. Thus, this represents a high potential risk of stored peanuts contamination with aflatoxins.
Fil: Passone, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Rosso, Laura Cristina. Consiglio Nazionale Delle Ricerche. Istituto Per la Protezione Sostenibile Delle Piante; Italia
Fil: Varela, María Celeste. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ciancio, A.. Consiglio Nazionale Delle Ricerche. Istituto Per la Protezione Sostenibile Delle Piante; Italia
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
description The aim of the work was to examine the effects of sub-lethal doses of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) (1+1 mmol/l and 5+5 mmol/l), water activities (0.98, 0.95, 0.93, 0.89 aW) and temperatures (28, 20 °C) on growth, sclerotial characteristics, aflatoxin accumulation and aflD (=nor-1) transcript level by Aspergillus parasiticus RCP08300 on peanut based medium. Growth rate and aflatoxin production were inhibited by BHA-BHT mixture (1+1 mmol/l), regardless of environmental factor assayed. Although sclerotia number and aflD expression were stimulated by this treatment, sclerotia dry weight and volume were reduced by 62.3 and 31.2%, respectively. In contrast, when the fungus grew in presence of the higher dose of BHA-BHT mixture none or very low aflatoxin accumulation and aflD expression occurred. Similarly, A. parasiticus growth has been highly influenced by BHA-BHT (5+5 mmol/l) and interacting stress factors. Data show that sub-lethal antioxidant doses significantly reduced growth and aflatoxin accumulation by A. parasiticus but these treatments were not able to repress the expression of the early expression gene (aflD) involved in aflatoxin biosynthesis. Thus, this represents a high potential risk of stored peanuts contamination with aflatoxins.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192900
Passone, Maria Alejandra; Rosso, Laura Cristina; Varela, María Celeste; Ciancio, A.; Etcheverry, Miriam Graciela; Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300; Wageningen Academic Publishers; World Mycotoxin Journal; 4; 2; 6-2011; 157-167
1875-0710
1875-0796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192900
identifier_str_mv Passone, Maria Alejandra; Rosso, Laura Cristina; Varela, María Celeste; Ciancio, A.; Etcheverry, Miriam Graciela; Effects of sub-lethal food grade antioxidant doses and environmental stressors on growth, sclerotia, aflatoxins and aflD (nor-1) expression by Aspergillus parasiticus RCP08300; Wageningen Academic Publishers; World Mycotoxin Journal; 4; 2; 6-2011; 157-167
1875-0710
1875-0796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2010.1261
info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.3920/WMJ2010.1261
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic Publishers
publisher.none.fl_str_mv Wageningen Academic Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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