Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent

Autores
Alaniz Zanon, Maria Silvina; Oddino, Claudio Marcelo; Giovanini, D.; Barbero, C.; Chiotta, María Laura; Chulze, Sofia Noemi
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Maize (Zea mays L.) is the cereal with the highest volume of production worldwide, and the second most important in Argentina. The presence of aflatoxins in the different stages of the maize agri-food chain is a current problem in food safety and it is caused by the contamination with species of Aspergillus section Flavi, mainly A. flavus. During many years the research group has focused on the development of a biological control strategy based on the competitive exclusion mechanism. Several studies have demonstrated the effectiveness of the non-toxicogenic A. flavus AFCHG2 strain developed by solid state fermentation on long grain rice. However, considering the United Nations sustainable development goal of zero hunger, it was proposed to replace this substrate and to develop a biopolymer that allows the growth and transport of the biological control agent to be applied to crops such as maize and peanuts. In this sense, different natural, economic and starch-rich substrates were analysed: cassava starch (10 and 15%), rice flour (10 and 15%) and maize starch (5, 10 and 15%). In addition, urea was added as a nitrogen source, and citric acid as promoter of greater crosslinking of starch chains. Also, the development of the biocontrol strain in polymers with the addition of glucose or sucrose was evaluated. The diameter of the pores of each polymer was determined and those with a pore diameter of 93-97 µm were selected assuming they allow a better use of the entire substrate by the biological control agent. In addition, the growth of the biological control strain in the different preparations was analysed. The synthesis of this biopolymer included stages of gelation, cooling, freezing, thawing, drying, sterilization and curing, hydration, pH regulation, inoculation, incubation and final drying. The effectiveness of the bioformulate evaluated under field showed a reduction of 81% in aflatoxin accumulation in maize kernels in comparison with the non-inoculated controls. The development of this biotechnological tool allowed us to present a process and product patent that is currently pending. In addition, it offers to producers an eco-friendly, economical and safe alternative that contributes to food quality and safety.
Fil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Oddino, Claudio Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Giovanini, D.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Barbero, C.. Universidad Nacional de Río Cuarto; Argentina
Fil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
World Mycotoxin Forum 13th Conference
Parma
Italia
World Mycotoxin Forum
Materia
MAIZE
AFLATOXINS
BIOCONTROL
BIOPOLYMER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228437
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228437
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agentAlaniz Zanon, Maria SilvinaOddino, Claudio MarceloGiovanini, D.Barbero, C.Chiotta, María LauraChulze, Sofia NoemiMAIZEAFLATOXINSBIOCONTROLBIOPOLYMERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Maize (Zea mays L.) is the cereal with the highest volume of production worldwide, and the second most important in Argentina. The presence of aflatoxins in the different stages of the maize agri-food chain is a current problem in food safety and it is caused by the contamination with species of Aspergillus section Flavi, mainly A. flavus. During many years the research group has focused on the development of a biological control strategy based on the competitive exclusion mechanism. Several studies have demonstrated the effectiveness of the non-toxicogenic A. flavus AFCHG2 strain developed by solid state fermentation on long grain rice. However, considering the United Nations sustainable development goal of zero hunger, it was proposed to replace this substrate and to develop a biopolymer that allows the growth and transport of the biological control agent to be applied to crops such as maize and peanuts. In this sense, different natural, economic and starch-rich substrates were analysed: cassava starch (10 and 15%), rice flour (10 and 15%) and maize starch (5, 10 and 15%). In addition, urea was added as a nitrogen source, and citric acid as promoter of greater crosslinking of starch chains. Also, the development of the biocontrol strain in polymers with the addition of glucose or sucrose was evaluated. The diameter of the pores of each polymer was determined and those with a pore diameter of 93-97 µm were selected assuming they allow a better use of the entire substrate by the biological control agent. In addition, the growth of the biological control strain in the different preparations was analysed. The synthesis of this biopolymer included stages of gelation, cooling, freezing, thawing, drying, sterilization and curing, hydration, pH regulation, inoculation, incubation and final drying. The effectiveness of the bioformulate evaluated under field showed a reduction of 81% in aflatoxin accumulation in maize kernels in comparison with the non-inoculated controls. The development of this biotechnological tool allowed us to present a process and product patent that is currently pending. In addition, it offers to producers an eco-friendly, economical and safe alternative that contributes to food quality and safety.Fil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Oddino, Claudio Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Giovanini, D.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Barbero, C.. Universidad Nacional de Río Cuarto; ArgentinaFil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaWorld Mycotoxin Forum 13th ConferenceParmaItaliaWorld Mycotoxin ForumWorld Mycotoxin Forum2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228437Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent; World Mycotoxin Forum 13th Conference; Parma; Italia; 2022; 136-137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.bastiaanse-communication.com/WMF2021-2022/media/book%20of%20abstracts%20WMFmeetsITALY%20final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:38Zoai:ri.conicet.gov.ar:11336/228437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:38.376CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
title Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
spellingShingle Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
Alaniz Zanon, Maria Silvina
MAIZE
AFLATOXINS
BIOCONTROL
BIOPOLYMER
title_short Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
title_full Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
title_fullStr Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
title_full_unstemmed Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
title_sort Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent
dc.creator.none.fl_str_mv Alaniz Zanon, Maria Silvina
Oddino, Claudio Marcelo
Giovanini, D.
Barbero, C.
Chiotta, María Laura
Chulze, Sofia Noemi
author Alaniz Zanon, Maria Silvina
author_facet Alaniz Zanon, Maria Silvina
Oddino, Claudio Marcelo
Giovanini, D.
Barbero, C.
Chiotta, María Laura
Chulze, Sofia Noemi
author_role author
author2 Oddino, Claudio Marcelo
Giovanini, D.
Barbero, C.
Chiotta, María Laura
Chulze, Sofia Noemi
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MAIZE
AFLATOXINS
BIOCONTROL
BIOPOLYMER
topic MAIZE
AFLATOXINS
BIOCONTROL
BIOPOLYMER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Maize (Zea mays L.) is the cereal with the highest volume of production worldwide, and the second most important in Argentina. The presence of aflatoxins in the different stages of the maize agri-food chain is a current problem in food safety and it is caused by the contamination with species of Aspergillus section Flavi, mainly A. flavus. During many years the research group has focused on the development of a biological control strategy based on the competitive exclusion mechanism. Several studies have demonstrated the effectiveness of the non-toxicogenic A. flavus AFCHG2 strain developed by solid state fermentation on long grain rice. However, considering the United Nations sustainable development goal of zero hunger, it was proposed to replace this substrate and to develop a biopolymer that allows the growth and transport of the biological control agent to be applied to crops such as maize and peanuts. In this sense, different natural, economic and starch-rich substrates were analysed: cassava starch (10 and 15%), rice flour (10 and 15%) and maize starch (5, 10 and 15%). In addition, urea was added as a nitrogen source, and citric acid as promoter of greater crosslinking of starch chains. Also, the development of the biocontrol strain in polymers with the addition of glucose or sucrose was evaluated. The diameter of the pores of each polymer was determined and those with a pore diameter of 93-97 µm were selected assuming they allow a better use of the entire substrate by the biological control agent. In addition, the growth of the biological control strain in the different preparations was analysed. The synthesis of this biopolymer included stages of gelation, cooling, freezing, thawing, drying, sterilization and curing, hydration, pH regulation, inoculation, incubation and final drying. The effectiveness of the bioformulate evaluated under field showed a reduction of 81% in aflatoxin accumulation in maize kernels in comparison with the non-inoculated controls. The development of this biotechnological tool allowed us to present a process and product patent that is currently pending. In addition, it offers to producers an eco-friendly, economical and safe alternative that contributes to food quality and safety.
Fil: Alaniz Zanon, Maria Silvina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Oddino, Claudio Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Giovanini, D.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Barbero, C.. Universidad Nacional de Río Cuarto; Argentina
Fil: Chiotta, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
World Mycotoxin Forum 13th Conference
Parma
Italia
World Mycotoxin Forum
description Maize (Zea mays L.) is the cereal with the highest volume of production worldwide, and the second most important in Argentina. The presence of aflatoxins in the different stages of the maize agri-food chain is a current problem in food safety and it is caused by the contamination with species of Aspergillus section Flavi, mainly A. flavus. During many years the research group has focused on the development of a biological control strategy based on the competitive exclusion mechanism. Several studies have demonstrated the effectiveness of the non-toxicogenic A. flavus AFCHG2 strain developed by solid state fermentation on long grain rice. However, considering the United Nations sustainable development goal of zero hunger, it was proposed to replace this substrate and to develop a biopolymer that allows the growth and transport of the biological control agent to be applied to crops such as maize and peanuts. In this sense, different natural, economic and starch-rich substrates were analysed: cassava starch (10 and 15%), rice flour (10 and 15%) and maize starch (5, 10 and 15%). In addition, urea was added as a nitrogen source, and citric acid as promoter of greater crosslinking of starch chains. Also, the development of the biocontrol strain in polymers with the addition of glucose or sucrose was evaluated. The diameter of the pores of each polymer was determined and those with a pore diameter of 93-97 µm were selected assuming they allow a better use of the entire substrate by the biological control agent. In addition, the growth of the biological control strain in the different preparations was analysed. The synthesis of this biopolymer included stages of gelation, cooling, freezing, thawing, drying, sterilization and curing, hydration, pH regulation, inoculation, incubation and final drying. The effectiveness of the bioformulate evaluated under field showed a reduction of 81% in aflatoxin accumulation in maize kernels in comparison with the non-inoculated controls. The development of this biotechnological tool allowed us to present a process and product patent that is currently pending. In addition, it offers to producers an eco-friendly, economical and safe alternative that contributes to food quality and safety.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228437
Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent; World Mycotoxin Forum 13th Conference; Parma; Italia; 2022; 136-137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228437
identifier_str_mv Bioformulate to reduce the accumulation of aflatoxins in maize based on a biopolymer as a carrier and support for growth of the biocontrol agent; World Mycotoxin Forum 13th Conference; Parma; Italia; 2022; 136-137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.bastiaanse-communication.com/WMF2021-2022/media/book%20of%20abstracts%20WMFmeetsITALY%20final.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv World Mycotoxin Forum
publisher.none.fl_str_mv World Mycotoxin Forum
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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