Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108

Autores
Passone, María Alejandra; Rosso, Laura Cristina; Etcheverry, Miriam Graciela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Effects of interacting conditions of sub-lethal levels of antioxidants, water potential (Ψ) and temperature were evaluated on growth, sclerotial characteristics, aflatoxin B 1 (AFB 1) production and aflD (=nor-1) gene expression by Aspergillus flavus strain RCP08108. These studies were carried out on peanut meal extract agar osmotically modified to -2.8,-7.1, -9.9 and -16.0MPa and incubated at 28 and 20°C. The food grade antioxidants added were butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) at (1+1mM-M1) and (5+5mM-M2). To relate the aflD expression after toxigenic A. flavus grew under interacting stress conditions, real-time PCR was used. Antioxidant mixtures caused a higher and significant (p<0.001) reduction in growth rate. The major impact on size and volume sclerotia was produced by Ψ; followed by antioxidant mixtures. High AFB 1 levels were observed in response to the M1 applied at -7.1MPa. Induction of the aflD gene was observed in response to the M1 treatment at -2.8, -7.1 and -9.9MPa; but significant decreases of AFB 1 production and aflD transcripts were observed; when the fungus grew in the presence of the M2 treatment.These results showed that it is necessary to apply food-grade antioxidants into the peanut storage system at levels higher than 5mM. This is an important tool to avoid sub-lethal antioxidant doses that can lead to fungal growth, increase resistance structures, and stimulate aflD gene expression and AFB 1 accumulation in this substrate.
Fil: Passone, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina
Fil: Rosso, Laura Cristina. Consiglio Nazionale delle Ricerche; Italia
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
AFLATOXINS
ANTIOXIDANTS
ASPERGILLUS FLAVUS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/198038
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/198038
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108Passone, María AlejandraRosso, Laura CristinaEtcheverry, Miriam GracielaAFLATOXINSANTIOXIDANTSASPERGILLUS FLAVUSENVIRONMENTAL FACTORSGROWTHSCLEROTIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Effects of interacting conditions of sub-lethal levels of antioxidants, water potential (Ψ) and temperature were evaluated on growth, sclerotial characteristics, aflatoxin B 1 (AFB 1) production and aflD (=nor-1) gene expression by Aspergillus flavus strain RCP08108. These studies were carried out on peanut meal extract agar osmotically modified to -2.8,-7.1, -9.9 and -16.0MPa and incubated at 28 and 20°C. The food grade antioxidants added were butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) at (1+1mM-M1) and (5+5mM-M2). To relate the aflD expression after toxigenic A. flavus grew under interacting stress conditions, real-time PCR was used. Antioxidant mixtures caused a higher and significant (p<0.001) reduction in growth rate. The major impact on size and volume sclerotia was produced by Ψ; followed by antioxidant mixtures. High AFB 1 levels were observed in response to the M1 applied at -7.1MPa. Induction of the aflD gene was observed in response to the M1 treatment at -2.8, -7.1 and -9.9MPa; but significant decreases of AFB 1 production and aflD transcripts were observed; when the fungus grew in the presence of the M2 treatment.These results showed that it is necessary to apply food-grade antioxidants into the peanut storage system at levels higher than 5mM. This is an important tool to avoid sub-lethal antioxidant doses that can lead to fungal growth, increase resistance structures, and stimulate aflD gene expression and AFB 1 accumulation in this substrate.Fil: Passone, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; ArgentinaFil: Rosso, Laura Cristina. Consiglio Nazionale delle Ricerche; ItaliaFil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaElsevier Gmbh2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/198038Passone, María Alejandra; Rosso, Laura Cristina; Etcheverry, Miriam Graciela; Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108; Elsevier Gmbh; Microbiological Research; 167; 8; 9-2012; 470-4770944-5013CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0944501311001030info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2011.11.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:07Zoai:ri.conicet.gov.ar:11336/198038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:07.344CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
title Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
spellingShingle Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
Passone, María Alejandra
AFLATOXINS
ANTIOXIDANTS
ASPERGILLUS FLAVUS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIA
title_short Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
title_full Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
title_fullStr Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
title_full_unstemmed Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
title_sort Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108
dc.creator.none.fl_str_mv Passone, María Alejandra
Rosso, Laura Cristina
Etcheverry, Miriam Graciela
author Passone, María Alejandra
author_facet Passone, María Alejandra
Rosso, Laura Cristina
Etcheverry, Miriam Graciela
author_role author
author2 Rosso, Laura Cristina
Etcheverry, Miriam Graciela
author2_role author
author
dc.subject.none.fl_str_mv AFLATOXINS
ANTIOXIDANTS
ASPERGILLUS FLAVUS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIA
topic AFLATOXINS
ANTIOXIDANTS
ASPERGILLUS FLAVUS
ENVIRONMENTAL FACTORS
GROWTH
SCLEROTIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Effects of interacting conditions of sub-lethal levels of antioxidants, water potential (Ψ) and temperature were evaluated on growth, sclerotial characteristics, aflatoxin B 1 (AFB 1) production and aflD (=nor-1) gene expression by Aspergillus flavus strain RCP08108. These studies were carried out on peanut meal extract agar osmotically modified to -2.8,-7.1, -9.9 and -16.0MPa and incubated at 28 and 20°C. The food grade antioxidants added were butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) at (1+1mM-M1) and (5+5mM-M2). To relate the aflD expression after toxigenic A. flavus grew under interacting stress conditions, real-time PCR was used. Antioxidant mixtures caused a higher and significant (p<0.001) reduction in growth rate. The major impact on size and volume sclerotia was produced by Ψ; followed by antioxidant mixtures. High AFB 1 levels were observed in response to the M1 applied at -7.1MPa. Induction of the aflD gene was observed in response to the M1 treatment at -2.8, -7.1 and -9.9MPa; but significant decreases of AFB 1 production and aflD transcripts were observed; when the fungus grew in the presence of the M2 treatment.These results showed that it is necessary to apply food-grade antioxidants into the peanut storage system at levels higher than 5mM. This is an important tool to avoid sub-lethal antioxidant doses that can lead to fungal growth, increase resistance structures, and stimulate aflD gene expression and AFB 1 accumulation in this substrate.
Fil: Passone, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina
Fil: Rosso, Laura Cristina. Consiglio Nazionale delle Ricerche; Italia
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Effects of interacting conditions of sub-lethal levels of antioxidants, water potential (Ψ) and temperature were evaluated on growth, sclerotial characteristics, aflatoxin B 1 (AFB 1) production and aflD (=nor-1) gene expression by Aspergillus flavus strain RCP08108. These studies were carried out on peanut meal extract agar osmotically modified to -2.8,-7.1, -9.9 and -16.0MPa and incubated at 28 and 20°C. The food grade antioxidants added were butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) at (1+1mM-M1) and (5+5mM-M2). To relate the aflD expression after toxigenic A. flavus grew under interacting stress conditions, real-time PCR was used. Antioxidant mixtures caused a higher and significant (p<0.001) reduction in growth rate. The major impact on size and volume sclerotia was produced by Ψ; followed by antioxidant mixtures. High AFB 1 levels were observed in response to the M1 applied at -7.1MPa. Induction of the aflD gene was observed in response to the M1 treatment at -2.8, -7.1 and -9.9MPa; but significant decreases of AFB 1 production and aflD transcripts were observed; when the fungus grew in the presence of the M2 treatment.These results showed that it is necessary to apply food-grade antioxidants into the peanut storage system at levels higher than 5mM. This is an important tool to avoid sub-lethal antioxidant doses that can lead to fungal growth, increase resistance structures, and stimulate aflD gene expression and AFB 1 accumulation in this substrate.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/198038
Passone, María Alejandra; Rosso, Laura Cristina; Etcheverry, Miriam Graciela; Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108; Elsevier Gmbh; Microbiological Research; 167; 8; 9-2012; 470-477
0944-5013
CONICET Digital
CONICET
url http://hdl.handle.net/11336/198038
identifier_str_mv Passone, María Alejandra; Rosso, Laura Cristina; Etcheverry, Miriam Graciela; Influence of sub-lethal doses, water potential and temperature on growth sclerotia, aflatoxins and aflD(=nor-1) expression by Aspergillus flavus RCPO8108; Elsevier Gmbh; Microbiological Research; 167; 8; 9-2012; 470-477
0944-5013
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0944501311001030
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2011.11.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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