Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco
- Autores
- Patricio, S.; Pereyra, Carina Maricel; Gonzalez, M. L.; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- There are few reports on the contamination of total aflatoxins (AFs), AFB1, deoxynivalenol (DON) and zearelenone (ZEA) in maize straw harvested in Mexico; hence, the aim of this study was to determine the concentration of such mycotoxins by immunoassay analytical technique (ELISA) and High-performance liquid chromatography (HPLC) in straw coming from 17 genotypes of white and yellow maize cultivated during 2009 in the municipality of Tepatitlan, Jalisco. Results showed a high percentage of occurrence of total aflatoxins by ELISA (88.2 %) and AFB1 by HPLC (84 %) with average levels of 7.0 ± 3.29 mg Kg-1 and 3.68 ± 2.91 mg Kg-1 respectively; DON determination by ELISA method presented 100 % of positive samples, average concentration was 6.04 ± 3.49 mg Kg-1; regarding the determination of ZEA by ELISA, 98.5 % of positive samples was observed with average levels of 1164.6 ± 1046.7 mg Kg-1 and by HPLC 81 % presented average levels of 544.6 ± 1035 mg Kg-1, no statistical difference was observed among straw of the different genotypes of maize (p>0.05) in any of the analyzed mycotoxins. It is important to mention that none of the samples exceeded the highest level recommended by the Mexican legislation for aflatoxins in feed for animals (20 mg Kg-1), however, 23.5 % (16/68) of the determined samples by HPLC surpassed the limit allowed for AFB1 by the European Community (5 mg Kg-1); 4.4 % (3/68) surpassed the levels allowed by DON (12 mg Kg-1); while ZEA showed 11.8 % (8/68) of samples detected for ELISA and 10.3 % (7/68) by HPLC with levels that exceed those recommended by the European Commission (2000 mg Kg-1), there is no regulation in Mexico for these mycotoxins.
Fil: Patricio, S.. Universidad de Guadalajara; México
Fil: Pereyra, Carina Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Gonzalez, M. L.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Maize straw
Aflatoxins
Deoxynivalenol
Zearelenone
ELISA
HPLC - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/161142
Ver los metadatos del registro completo
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Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, JaliscoAflatoxinas, deoxinivalenol y zearalenona en rastrojo de maíz cosechado en Tepatitlán, JaliscoPatricio, S.Pereyra, Carina MaricelGonzalez, M. L.Cavaglieri, Lilia ReneéDalcero, Ana MariaMaize strawAflatoxinsDeoxynivalenolZearelenoneELISAHPLChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1There are few reports on the contamination of total aflatoxins (AFs), AFB1, deoxynivalenol (DON) and zearelenone (ZEA) in maize straw harvested in Mexico; hence, the aim of this study was to determine the concentration of such mycotoxins by immunoassay analytical technique (ELISA) and High-performance liquid chromatography (HPLC) in straw coming from 17 genotypes of white and yellow maize cultivated during 2009 in the municipality of Tepatitlan, Jalisco. Results showed a high percentage of occurrence of total aflatoxins by ELISA (88.2 %) and AFB1 by HPLC (84 %) with average levels of 7.0 ± 3.29 mg Kg-1 and 3.68 ± 2.91 mg Kg-1 respectively; DON determination by ELISA method presented 100 % of positive samples, average concentration was 6.04 ± 3.49 mg Kg-1; regarding the determination of ZEA by ELISA, 98.5 % of positive samples was observed with average levels of 1164.6 ± 1046.7 mg Kg-1 and by HPLC 81 % presented average levels of 544.6 ± 1035 mg Kg-1, no statistical difference was observed among straw of the different genotypes of maize (p>0.05) in any of the analyzed mycotoxins. It is important to mention that none of the samples exceeded the highest level recommended by the Mexican legislation for aflatoxins in feed for animals (20 mg Kg-1), however, 23.5 % (16/68) of the determined samples by HPLC surpassed the limit allowed for AFB1 by the European Community (5 mg Kg-1); 4.4 % (3/68) surpassed the levels allowed by DON (12 mg Kg-1); while ZEA showed 11.8 % (8/68) of samples detected for ELISA and 10.3 % (7/68) by HPLC with levels that exceed those recommended by the European Commission (2000 mg Kg-1), there is no regulation in Mexico for these mycotoxins.Fil: Patricio, S.. Universidad de Guadalajara; MéxicoFil: Pereyra, Carina Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Gonzalez, M. L.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaUniversidad Autónoma de Nayarit2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161142Patricio, S.; Pereyra, Carina Maricel; Gonzalez, M. L.; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria; Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco; Universidad Autónoma de Nayarit; Revista Bio-Ciencias; 4; 1; 5-2016; 3-142007-3380CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://revistabiociencias.uan.edu.mx/index.php/BIOCIENCIAS/issue/view/26/showTocinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:34Zoai:ri.conicet.gov.ar:11336/161142instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:34.599CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco Aflatoxinas, deoxinivalenol y zearalenona en rastrojo de maíz cosechado en Tepatitlán, Jalisco |
| title |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco |
| spellingShingle |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco Patricio, S. Maize straw Aflatoxins Deoxynivalenol Zearelenone ELISA HPLC |
| title_short |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco |
| title_full |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco |
| title_fullStr |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco |
| title_full_unstemmed |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco |
| title_sort |
Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco |
| dc.creator.none.fl_str_mv |
Patricio, S. Pereyra, Carina Maricel Gonzalez, M. L. Cavaglieri, Lilia Reneé Dalcero, Ana Maria |
| author |
Patricio, S. |
| author_facet |
Patricio, S. Pereyra, Carina Maricel Gonzalez, M. L. Cavaglieri, Lilia Reneé Dalcero, Ana Maria |
| author_role |
author |
| author2 |
Pereyra, Carina Maricel Gonzalez, M. L. Cavaglieri, Lilia Reneé Dalcero, Ana Maria |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Maize straw Aflatoxins Deoxynivalenol Zearelenone ELISA HPLC |
| topic |
Maize straw Aflatoxins Deoxynivalenol Zearelenone ELISA HPLC |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
There are few reports on the contamination of total aflatoxins (AFs), AFB1, deoxynivalenol (DON) and zearelenone (ZEA) in maize straw harvested in Mexico; hence, the aim of this study was to determine the concentration of such mycotoxins by immunoassay analytical technique (ELISA) and High-performance liquid chromatography (HPLC) in straw coming from 17 genotypes of white and yellow maize cultivated during 2009 in the municipality of Tepatitlan, Jalisco. Results showed a high percentage of occurrence of total aflatoxins by ELISA (88.2 %) and AFB1 by HPLC (84 %) with average levels of 7.0 ± 3.29 mg Kg-1 and 3.68 ± 2.91 mg Kg-1 respectively; DON determination by ELISA method presented 100 % of positive samples, average concentration was 6.04 ± 3.49 mg Kg-1; regarding the determination of ZEA by ELISA, 98.5 % of positive samples was observed with average levels of 1164.6 ± 1046.7 mg Kg-1 and by HPLC 81 % presented average levels of 544.6 ± 1035 mg Kg-1, no statistical difference was observed among straw of the different genotypes of maize (p>0.05) in any of the analyzed mycotoxins. It is important to mention that none of the samples exceeded the highest level recommended by the Mexican legislation for aflatoxins in feed for animals (20 mg Kg-1), however, 23.5 % (16/68) of the determined samples by HPLC surpassed the limit allowed for AFB1 by the European Community (5 mg Kg-1); 4.4 % (3/68) surpassed the levels allowed by DON (12 mg Kg-1); while ZEA showed 11.8 % (8/68) of samples detected for ELISA and 10.3 % (7/68) by HPLC with levels that exceed those recommended by the European Commission (2000 mg Kg-1), there is no regulation in Mexico for these mycotoxins. Fil: Patricio, S.. Universidad de Guadalajara; México Fil: Pereyra, Carina Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Gonzalez, M. L.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
There are few reports on the contamination of total aflatoxins (AFs), AFB1, deoxynivalenol (DON) and zearelenone (ZEA) in maize straw harvested in Mexico; hence, the aim of this study was to determine the concentration of such mycotoxins by immunoassay analytical technique (ELISA) and High-performance liquid chromatography (HPLC) in straw coming from 17 genotypes of white and yellow maize cultivated during 2009 in the municipality of Tepatitlan, Jalisco. Results showed a high percentage of occurrence of total aflatoxins by ELISA (88.2 %) and AFB1 by HPLC (84 %) with average levels of 7.0 ± 3.29 mg Kg-1 and 3.68 ± 2.91 mg Kg-1 respectively; DON determination by ELISA method presented 100 % of positive samples, average concentration was 6.04 ± 3.49 mg Kg-1; regarding the determination of ZEA by ELISA, 98.5 % of positive samples was observed with average levels of 1164.6 ± 1046.7 mg Kg-1 and by HPLC 81 % presented average levels of 544.6 ± 1035 mg Kg-1, no statistical difference was observed among straw of the different genotypes of maize (p>0.05) in any of the analyzed mycotoxins. It is important to mention that none of the samples exceeded the highest level recommended by the Mexican legislation for aflatoxins in feed for animals (20 mg Kg-1), however, 23.5 % (16/68) of the determined samples by HPLC surpassed the limit allowed for AFB1 by the European Community (5 mg Kg-1); 4.4 % (3/68) surpassed the levels allowed by DON (12 mg Kg-1); while ZEA showed 11.8 % (8/68) of samples detected for ELISA and 10.3 % (7/68) by HPLC with levels that exceed those recommended by the European Commission (2000 mg Kg-1), there is no regulation in Mexico for these mycotoxins. |
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2016 |
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2016-05 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/161142 Patricio, S.; Pereyra, Carina Maricel; Gonzalez, M. L.; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria; Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco; Universidad Autónoma de Nayarit; Revista Bio-Ciencias; 4; 1; 5-2016; 3-14 2007-3380 CONICET Digital CONICET |
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http://hdl.handle.net/11336/161142 |
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Patricio, S.; Pereyra, Carina Maricel; Gonzalez, M. L.; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria; Aflatoxins, deoxynivalenol and zearalenone in maize straw harvested in Tepatitlan, Jalisco; Universidad Autónoma de Nayarit; Revista Bio-Ciencias; 4; 1; 5-2016; 3-14 2007-3380 CONICET Digital CONICET |
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