Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn

Autores
Astoreca, Andrea Luciana; Vaamonde, Graciela; Dalcero, Ana Maria; Marin, Sonia; Ramos, Antonio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB1+/CPA+), III (AFB1+/CPA-) and IV (AFB1-/CPA+), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different temperatures (10-40 ºC) and aW levels (0.80-0.98). AFB1 and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB1 production occurred more favourably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aW evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB1 production occurred at 0.96 aW and 30 ºC after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to design control strategies of these mycotoxins in corn.
Fil: Astoreca, Andrea Luciana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); Argentina
Fil: Vaamonde, Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología. Cátedra de Micología; Argentina
Fil: Marin, Sonia. Universitat de Lleida. Departament de Tecnologia d’Aliments; España
Fil: Ramos, Antonio. Universitat de Lleida. Departament de Tecnologia d’Aliments; España
Materia
Abiotic Factors
Aspergillus Flavus
Aflatoxins
Cyclopiazonic Acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4322
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4322
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from cornAstoreca, Andrea LucianaVaamonde, GracielaDalcero, Ana MariaMarin, SoniaRamos, AntonioAbiotic FactorsAspergillus FlavusAflatoxinsCyclopiazonic Acidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB1+/CPA+), III (AFB1+/CPA-) and IV (AFB1-/CPA+), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different temperatures (10-40 ºC) and aW levels (0.80-0.98). AFB1 and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB1 production occurred more favourably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aW evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB1 production occurred at 0.96 aW and 30 ºC after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to design control strategies of these mycotoxins in corn.Fil: Astoreca, Andrea Luciana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); ArgentinaFil: Vaamonde, Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología. Cátedra de Micología; ArgentinaFil: Marin, Sonia. Universitat de Lleida. Departament de Tecnologia d’Aliments; EspañaFil: Ramos, Antonio. Universitat de Lleida. Departament de Tecnologia d’Aliments; EspañaElsevier2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4322Astoreca, Andrea Luciana; Vaamonde, Graciela; Dalcero, Ana Maria; Marin, Sonia; Ramos, Antonio; Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn; Elsevier; Food Microbiology; 38; 7-2013; 276-2830740-0020enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0740002013001512info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fm.2013.07.012info:eu-repo/semantics/altIdentifier/issn/0740-0020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:18Zoai:ri.conicet.gov.ar:11336/4322instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:18.513CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
title Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
spellingShingle Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
Astoreca, Andrea Luciana
Abiotic Factors
Aspergillus Flavus
Aflatoxins
Cyclopiazonic Acid
title_short Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
title_full Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
title_fullStr Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
title_full_unstemmed Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
title_sort Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn
dc.creator.none.fl_str_mv Astoreca, Andrea Luciana
Vaamonde, Graciela
Dalcero, Ana Maria
Marin, Sonia
Ramos, Antonio
author Astoreca, Andrea Luciana
author_facet Astoreca, Andrea Luciana
Vaamonde, Graciela
Dalcero, Ana Maria
Marin, Sonia
Ramos, Antonio
author_role author
author2 Vaamonde, Graciela
Dalcero, Ana Maria
Marin, Sonia
Ramos, Antonio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Abiotic Factors
Aspergillus Flavus
Aflatoxins
Cyclopiazonic Acid
topic Abiotic Factors
Aspergillus Flavus
Aflatoxins
Cyclopiazonic Acid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB1+/CPA+), III (AFB1+/CPA-) and IV (AFB1-/CPA+), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different temperatures (10-40 ºC) and aW levels (0.80-0.98). AFB1 and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB1 production occurred more favourably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aW evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB1 production occurred at 0.96 aW and 30 ºC after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to design control strategies of these mycotoxins in corn.
Fil: Astoreca, Andrea Luciana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); Argentina
Fil: Vaamonde, Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología E Inmunología. Cátedra de Micología; Argentina
Fil: Marin, Sonia. Universitat de Lleida. Departament de Tecnologia d’Aliments; España
Fil: Ramos, Antonio. Universitat de Lleida. Departament de Tecnologia d’Aliments; España
description The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB1+/CPA+), III (AFB1+/CPA-) and IV (AFB1-/CPA+), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different temperatures (10-40 ºC) and aW levels (0.80-0.98). AFB1 and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB1 production occurred more favourably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aW evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB1 production occurred at 0.96 aW and 30 ºC after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to design control strategies of these mycotoxins in corn.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4322
Astoreca, Andrea Luciana; Vaamonde, Graciela; Dalcero, Ana Maria; Marin, Sonia; Ramos, Antonio; Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn; Elsevier; Food Microbiology; 38; 7-2013; 276-283
0740-0020
url http://hdl.handle.net/11336/4322
identifier_str_mv Astoreca, Andrea Luciana; Vaamonde, Graciela; Dalcero, Ana Maria; Marin, Sonia; Ramos, Antonio; Abiotic factors and their interactions influence on the co-production of aflatoxins and cyclopiazonic acid by strains of Aspergillus flavus isolated from corn; Elsevier; Food Microbiology; 38; 7-2013; 276-283
0740-0020
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0740002013001512
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fm.2013.07.012
info:eu-repo/semantics/altIdentifier/issn/0740-0020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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