Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
- Autores
- Collavino, Mónica M.; Ramos Cabrera, Efrén Venancio; Bruno, Cecilia; Aguilar, Orlando Mario
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
Instituto de Biotecnología y Biología Molecular - Materia
-
Biología
Endophytes
N2-fixing bacteria
Tomato
nifH gene pyrosequencing - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/142827
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Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growthCollavino, Mónica M.Ramos Cabrera, Efrén VenancioBruno, CeciliaAguilar, Orlando MarioBiologíaEndophytesN2-fixing bacteriaTomatonifH gene pyrosequencingThe aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.Instituto de Biotecnología y Biología Molecular2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1965-1975http://sedici.unlp.edu.ar/handle/10915/142827enginfo:eu-repo/semantics/altIdentifier/issn/1678-4405info:eu-repo/semantics/altIdentifier/issn/1517-8382info:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3info:eu-repo/semantics/altIdentifier/pmid/32895888info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:32Zoai:sedici.unlp.edu.ar:10915/142827Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:33.014SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
title |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
spellingShingle |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth Collavino, Mónica M. Biología Endophytes N2-fixing bacteria Tomato nifH gene pyrosequencing |
title_short |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
title_full |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
title_fullStr |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
title_full_unstemmed |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
title_sort |
Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth |
dc.creator.none.fl_str_mv |
Collavino, Mónica M. Ramos Cabrera, Efrén Venancio Bruno, Cecilia Aguilar, Orlando Mario |
author |
Collavino, Mónica M. |
author_facet |
Collavino, Mónica M. Ramos Cabrera, Efrén Venancio Bruno, Cecilia Aguilar, Orlando Mario |
author_role |
author |
author2 |
Ramos Cabrera, Efrén Venancio Bruno, Cecilia Aguilar, Orlando Mario |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Biología Endophytes N2-fixing bacteria Tomato nifH gene pyrosequencing |
topic |
Biología Endophytes N2-fixing bacteria Tomato nifH gene pyrosequencing |
dc.description.none.fl_txt_mv |
The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops. Instituto de Biotecnología y Biología Molecular |
description |
The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
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publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/142827 |
url |
http://sedici.unlp.edu.ar/handle/10915/142827 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1678-4405 info:eu-repo/semantics/altIdentifier/issn/1517-8382 info:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3 info:eu-repo/semantics/altIdentifier/pmid/32895888 |
dc.rights.none.fl_str_mv |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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