Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth

Autores
Collavino, Mónica M.; Ramos Cabrera, Efrén Venancio; Bruno, Cecilia; Aguilar, Orlando Mario
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
Instituto de Biotecnología y Biología Molecular
Materia
Biología
Endophytes
N2-fixing bacteria
Tomato
nifH gene pyrosequencing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/142827

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spelling Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growthCollavino, Mónica M.Ramos Cabrera, Efrén VenancioBruno, CeciliaAguilar, Orlando MarioBiologíaEndophytesN2-fixing bacteriaTomatonifH gene pyrosequencingThe aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.Instituto de Biotecnología y Biología Molecular2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1965-1975http://sedici.unlp.edu.ar/handle/10915/142827enginfo:eu-repo/semantics/altIdentifier/issn/1678-4405info:eu-repo/semantics/altIdentifier/issn/1517-8382info:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3info:eu-repo/semantics/altIdentifier/pmid/32895888info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:32Zoai:sedici.unlp.edu.ar:10915/142827Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:33.014SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
spellingShingle Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
Collavino, Mónica M.
Biología
Endophytes
N2-fixing bacteria
Tomato
nifH gene pyrosequencing
title_short Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_full Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_fullStr Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_full_unstemmed Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_sort Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
dc.creator.none.fl_str_mv Collavino, Mónica M.
Ramos Cabrera, Efrén Venancio
Bruno, Cecilia
Aguilar, Orlando Mario
author Collavino, Mónica M.
author_facet Collavino, Mónica M.
Ramos Cabrera, Efrén Venancio
Bruno, Cecilia
Aguilar, Orlando Mario
author_role author
author2 Ramos Cabrera, Efrén Venancio
Bruno, Cecilia
Aguilar, Orlando Mario
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Endophytes
N2-fixing bacteria
Tomato
nifH gene pyrosequencing
topic Biología
Endophytes
N2-fixing bacteria
Tomato
nifH gene pyrosequencing
dc.description.none.fl_txt_mv The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
Instituto de Biotecnología y Biología Molecular
description The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization.Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/142827
url http://sedici.unlp.edu.ar/handle/10915/142827
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1678-4405
info:eu-repo/semantics/altIdentifier/issn/1517-8382
info:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3
info:eu-repo/semantics/altIdentifier/pmid/32895888
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1965-1975
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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