Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth

Autores
Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Ramos Cabrera, Efren Venancio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Bruno, Cecilia Ines. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146046
Acceder
id CONICETDig_a5cb4e2fe5f467f25bfb8ecbdd37cf32
oai_identifier_str oai:ri.conicet.gov.ar:11336/146046
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growthCollavino, Mónica MarianaRamos Cabrera, Efren VenancioBruno, Cecilia InesAguilar, Orlando MarioENDOPHYTESN2-FIXING BACTERIANIFH GENE PYROSEQUENCINGTOMATOhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Ramos Cabrera, Efren Venancio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Bruno, Cecilia Ines. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; ArgentinaFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaSpringer2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146046Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-19751517-83821678-4405CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs42770-020-00373-3info:eu-repo/semantics/altIdentifier/url/https://europepmc.org/article/med/32895888#free-full-textinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:22Zoai:ri.conicet.gov.ar:11336/146046instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:22.686CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
spellingShingle Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
Collavino, Mónica Mariana
ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
title_short Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_full Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_fullStr Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_full_unstemmed Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_sort Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
dc.creator.none.fl_str_mv Collavino, Mónica Mariana
Ramos Cabrera, Efren Venancio
Bruno, Cecilia Ines
Aguilar, Orlando Mario
author Collavino, Mónica Mariana
author_facet Collavino, Mónica Mariana
Ramos Cabrera, Efren Venancio
Bruno, Cecilia Ines
Aguilar, Orlando Mario
author_role author
author2 Ramos Cabrera, Efren Venancio
Bruno, Cecilia Ines
Aguilar, Orlando Mario
author2_role author
author
author
dc.subject.none.fl_str_mv ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
topic ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Ramos Cabrera, Efren Venancio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Bruno, Cecilia Ines. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146046
Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-1975
1517-8382
1678-4405
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146046
identifier_str_mv Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-1975
1517-8382
1678-4405
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs42770-020-00373-3
info:eu-repo/semantics/altIdentifier/url/https://europepmc.org/article/med/32895888#free-full-text
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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