nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics

Autores
Collavino, Mónica Mariana; Tripp, H. James; Frank, Ildiko E.; Vidoz, María Laura; Calderoli, Priscila Anabel; Donato, Mariano Humberto; Zehr, Jonathan P.; Aguilar, Orlando Mario
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A dataset of 87 020 nifH reads and 16 782 unique nifH protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of Alphaproteobacteria, Betaproteobacteria and Deltaproteobacteria, mainly relatives to Bradyrhizobium and Methylosinus/Methylocystis, but a surprising paucity of Gammaproteobacteria. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on nifH diversity. nifH gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Facultad de Ciencias Naturales y Museo
Laboratorio de Sistemática y Biología Evolutiva
Materia
Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127286

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/127286
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamicsCollavino, Mónica MarianaTripp, H. JamesFrank, Ildiko E.Vidoz, María LauraCalderoli, Priscila AnabelDonato, Mariano HumbertoZehr, Jonathan P.Aguilar, Orlando MarioCiencias NaturalesBiologíanifH pyrosequencingsoil chemistryphylogenetic analysisBacteriaA dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of <i>Alphaproteobacteria</i>, <i>Betaproteobacteria</i> and <i>Deltaproteobacteria</i>, mainly relatives to <i>Bradyrhizobium</i> and <i>Methylosinus/Methylocystis</i>, but a surprising paucity of <i>Gammaproteobacteria</i>. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on <i>nifH</i> diversity. <i>nifH</i> gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Naturales y MuseoLaboratorio de Sistemática y Biología Evolutiva2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf3211-3223http://sedici.unlp.edu.ar/handle/10915/127286enginfo:eu-repo/semantics/altIdentifier/issn/1462-2920info:eu-repo/semantics/altIdentifier/issn/1462-2912info:eu-repo/semantics/altIdentifier/pmid/25314671info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.12423info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:42Zoai:sedici.unlp.edu.ar:10915/127286Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:43.198SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
spellingShingle nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
Collavino, Mónica Mariana
Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
title_short nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_full nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_fullStr nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_full_unstemmed nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_sort nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
dc.creator.none.fl_str_mv Collavino, Mónica Mariana
Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author Collavino, Mónica Mariana
author_facet Collavino, Mónica Mariana
Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author_role author
author2 Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
topic Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
dc.description.none.fl_txt_mv A dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of <i>Alphaproteobacteria</i>, <i>Betaproteobacteria</i> and <i>Deltaproteobacteria</i>, mainly relatives to <i>Bradyrhizobium</i> and <i>Methylosinus/Methylocystis</i>, but a surprising paucity of <i>Gammaproteobacteria</i>. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on <i>nifH</i> diversity. <i>nifH</i> gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Facultad de Ciencias Naturales y Museo
Laboratorio de Sistemática y Biología Evolutiva
description A dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of <i>Alphaproteobacteria</i>, <i>Betaproteobacteria</i> and <i>Deltaproteobacteria</i>, mainly relatives to <i>Bradyrhizobium</i> and <i>Methylosinus/Methylocystis</i>, but a surprising paucity of <i>Gammaproteobacteria</i>. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on <i>nifH</i> diversity. <i>nifH</i> gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127286
url http://sedici.unlp.edu.ar/handle/10915/127286
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1462-2920
info:eu-repo/semantics/altIdentifier/issn/1462-2912
info:eu-repo/semantics/altIdentifier/pmid/25314671
info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.12423
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
3211-3223
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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