nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
- Autores
- Collavino, Mónica Mariana; Tripp, H. James; Frank, Ildiko E.; Vidoz, María Laura; Calderoli, Priscila Anabel; Donato, Mariano Humberto; Zehr, Jonathan P.; Aguilar, Orlando Mario
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A dataset of 87 020 nifH reads and 16 782 unique nifH protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of Alphaproteobacteria, Betaproteobacteria and Deltaproteobacteria, mainly relatives to Bradyrhizobium and Methylosinus/Methylocystis, but a surprising paucity of Gammaproteobacteria. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on nifH diversity. nifH gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Facultad de Ciencias Naturales y Museo
Laboratorio de Sistemática y Biología Evolutiva - Materia
-
Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/127286
Ver los metadatos del registro completo
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nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamicsCollavino, Mónica MarianaTripp, H. JamesFrank, Ildiko E.Vidoz, María LauraCalderoli, Priscila AnabelDonato, Mariano HumbertoZehr, Jonathan P.Aguilar, Orlando MarioCiencias NaturalesBiologíanifH pyrosequencingsoil chemistryphylogenetic analysisBacteriaA dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of <i>Alphaproteobacteria</i>, <i>Betaproteobacteria</i> and <i>Deltaproteobacteria</i>, mainly relatives to <i>Bradyrhizobium</i> and <i>Methylosinus/Methylocystis</i>, but a surprising paucity of <i>Gammaproteobacteria</i>. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on <i>nifH</i> diversity. <i>nifH</i> gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Naturales y MuseoLaboratorio de Sistemática y Biología Evolutiva2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf3211-3223http://sedici.unlp.edu.ar/handle/10915/127286enginfo:eu-repo/semantics/altIdentifier/issn/1462-2920info:eu-repo/semantics/altIdentifier/issn/1462-2912info:eu-repo/semantics/altIdentifier/pmid/25314671info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.12423info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:22:31Zoai:sedici.unlp.edu.ar:10915/127286Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:22:31.838SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics |
| title |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics |
| spellingShingle |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics Collavino, Mónica Mariana Ciencias Naturales Biología nifH pyrosequencing soil chemistry phylogenetic analysis Bacteria |
| title_short |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics |
| title_full |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics |
| title_fullStr |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics |
| title_full_unstemmed |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics |
| title_sort |
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics |
| dc.creator.none.fl_str_mv |
Collavino, Mónica Mariana Tripp, H. James Frank, Ildiko E. Vidoz, María Laura Calderoli, Priscila Anabel Donato, Mariano Humberto Zehr, Jonathan P. Aguilar, Orlando Mario |
| author |
Collavino, Mónica Mariana |
| author_facet |
Collavino, Mónica Mariana Tripp, H. James Frank, Ildiko E. Vidoz, María Laura Calderoli, Priscila Anabel Donato, Mariano Humberto Zehr, Jonathan P. Aguilar, Orlando Mario |
| author_role |
author |
| author2 |
Tripp, H. James Frank, Ildiko E. Vidoz, María Laura Calderoli, Priscila Anabel Donato, Mariano Humberto Zehr, Jonathan P. Aguilar, Orlando Mario |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Naturales Biología nifH pyrosequencing soil chemistry phylogenetic analysis Bacteria |
| topic |
Ciencias Naturales Biología nifH pyrosequencing soil chemistry phylogenetic analysis Bacteria |
| dc.description.none.fl_txt_mv |
A dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of <i>Alphaproteobacteria</i>, <i>Betaproteobacteria</i> and <i>Deltaproteobacteria</i>, mainly relatives to <i>Bradyrhizobium</i> and <i>Methylosinus/Methylocystis</i>, but a surprising paucity of <i>Gammaproteobacteria</i>. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on <i>nifH</i> diversity. <i>nifH</i> gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria. Facultad de Ciencias Exactas Instituto de Biotecnologia y Biologia Molecular Facultad de Ciencias Naturales y Museo Laboratorio de Sistemática y Biología Evolutiva |
| description |
A dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of <i>Alphaproteobacteria</i>, <i>Betaproteobacteria</i> and <i>Deltaproteobacteria</i>, mainly relatives to <i>Bradyrhizobium</i> and <i>Methylosinus/Methylocystis</i>, but a surprising paucity of <i>Gammaproteobacteria</i>. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on <i>nifH</i> diversity. <i>nifH</i> gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria. |
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2014 |
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2014-10 |
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eng |
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