Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use...

Autores: Calderoli, Priscila A.; Collavino, Mónica Mariana; Behrends Kraemer, Filipe; Morrás, Héctor J. M.; Aguilar, Mario O.
Año de publicación: 2017
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Fil: Calderoli, Priscila A. Universidad Nacional de La Plata; Argentina.
Fil: Calderoli, Priscila A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Manejo y Conservación de Suelos; Argentina.
Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina.
Fil: Morrás, Héctor J. M. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina.
Fil: Aguilar, Mario O. Universidad Nacional de La Plata; Argentina.
Fil: Aguilar, Mario O. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina.
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbón might contribute to the functional signatures of particular nifH phylotypes in fieldsunder no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
Fuente: MicrobiologyOpen, 2017, vol. 6, no. 5, p. 1-15.
Materia: Cyanobacteria and Geobacter
NifH transcripts
Soil diazotrophic community
Soil management
Practices
Nivel de accesibilidad: acceso abierto
Condiciones de uso: http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Institución: Universidad Nacional del Nordeste
OAI Identificador: oai:repositorio.unne.edu.ar:123456789/27726

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network_acronym_str	RIUNNE
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network_name_str	Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soilCalderoli, Priscila A.Collavino, Mónica MarianaBehrends Kraemer, FilipeMorrás, Héctor J. M.Aguilar, Mario O.Cyanobacteria and GeobacterNifH transcriptsSoil diazotrophic communitySoil managementPracticesFil: Calderoli, Priscila A. Universidad Nacional de La Plata; Argentina.Fil: Calderoli, Priscila A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Manejo y Conservación de Suelos; Argentina.Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina.Fil: Morrás, Héctor J. M. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina.Fil: Aguilar, Mario O. Universidad Nacional de La Plata; Argentina.Fil: Aguilar, Mario O. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina.In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbón might contribute to the functional signatures of particular nifH phylotypes in fieldsunder no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.Blackwell Publishing2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfCalderoli, Priscila A., et al., 2017. Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil. MicrobiologyOpen. Oxford: Blackwell Publishing, vol. 6, no. 5, p. 1-15. ISSN 2045-8827.2045-8827http://repositorio.unne.edu.ar/handle/123456789/27726MicrobiologyOpen, 2017, vol. 6, no. 5, p. 1-15.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1002/mbo3.502https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.502info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:29:50Zoai:repositorio.unne.edu.ar:123456789/27726instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:29:50.445Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
spellingShingle	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil Calderoli, Priscila A. Cyanobacteria and Geobacter NifH transcripts Soil diazotrophic community Soil management Practices
title_short	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_full	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_fullStr	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_full_unstemmed	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_sort	Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
dc.creator.none.fl_str_mv	Calderoli, Priscila A. Collavino, Mónica Mariana Behrends Kraemer, Filipe Morrás, Héctor J. M. Aguilar, Mario O.
author	Calderoli, Priscila A.
author_facet	Calderoli, Priscila A. Collavino, Mónica Mariana Behrends Kraemer, Filipe Morrás, Héctor J. M. Aguilar, Mario O.
author_role	author
author2	Collavino, Mónica Mariana Behrends Kraemer, Filipe Morrás, Héctor J. M. Aguilar, Mario O.
author2_role	author author author author
dc.subject.none.fl_str_mv	Cyanobacteria and Geobacter NifH transcripts Soil diazotrophic community Soil management Practices
topic	Cyanobacteria and Geobacter NifH transcripts Soil diazotrophic community Soil management Practices
dc.description.none.fl_txt_mv	Fil: Calderoli, Priscila A. Universidad Nacional de La Plata; Argentina. Fil: Calderoli, Priscila A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Manejo y Conservación de Suelos; Argentina. Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina. Fil: Morrás, Héctor J. M. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina. Fil: Aguilar, Mario O. Universidad Nacional de La Plata; Argentina. Fil: Aguilar, Mario O. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbón might contribute to the functional signatures of particular nifH phylotypes in fieldsunder no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
description	Fil: Calderoli, Priscila A. Universidad Nacional de La Plata; Argentina.
publishDate	2017
dc.date.none.fl_str_mv	2017-05
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	Calderoli, Priscila A., et al., 2017. Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil. MicrobiologyOpen. Oxford: Blackwell Publishing, vol. 6, no. 5, p. 1-15. ISSN 2045-8827. 2045-8827 http://repositorio.unne.edu.ar/handle/123456789/27726
identifier_str_mv	Calderoli, Priscila A., et al., 2017. Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil. MicrobiologyOpen. Oxford: Blackwell Publishing, vol. 6, no. 5, p. 1-15. ISSN 2045-8827. 2045-8827
url	http://repositorio.unne.edu.ar/handle/123456789/27726
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	https://doi.org/10.1002/mbo3.502 https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.502
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv	openAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Blackwell Publishing
publisher.none.fl_str_mv	Blackwell Publishing
dc.source.none.fl_str_mv	MicrobiologyOpen, 2017, vol. 6, no. 5, p. 1-15. reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) instname:Universidad Nacional del Nordeste
reponame_str	Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection	Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str	Universidad Nacional del Nordeste
repository.name.fl_str_mv	Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv	ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score	12.559606

Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use...

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