Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agric...
- Autores
- Calderoli, Priscila Anabel; Collavino, Mónica Mariana; Behrends Kraemer, Filipe; Morrás, Héctor J. M.; Aguilar, Orlando Mario
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
Instituto de Biotecnologia y Biologia Molecular - Materia
-
Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/87448
Ver los metadatos del registro completo
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Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soilCalderoli, Priscila AnabelCollavino, Mónica MarianaBehrends Kraemer, FilipeMorrás, Héctor J. M.Aguilar, Orlando MarioBioquímicaCyanobacteria and GeobacternifH transcriptssoil diazotrophic communitysoil management practicesIn this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N<sub>2</sub> fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.Instituto de Biotecnologia y Biologia Molecular2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87448enginfo:eu-repo/semantics/altIdentifier/issn/2045-8827info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.502info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:10Zoai:sedici.unlp.edu.ar:10915/87448Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:10.353SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil |
title |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil |
spellingShingle |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil Calderoli, Priscila Anabel Bioquímica Cyanobacteria and Geobacter nifH transcripts soil diazotrophic community soil management practices |
title_short |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil |
title_full |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil |
title_fullStr |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil |
title_full_unstemmed |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil |
title_sort |
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil |
dc.creator.none.fl_str_mv |
Calderoli, Priscila Anabel Collavino, Mónica Mariana Behrends Kraemer, Filipe Morrás, Héctor J. M. Aguilar, Orlando Mario |
author |
Calderoli, Priscila Anabel |
author_facet |
Calderoli, Priscila Anabel Collavino, Mónica Mariana Behrends Kraemer, Filipe Morrás, Héctor J. M. Aguilar, Orlando Mario |
author_role |
author |
author2 |
Collavino, Mónica Mariana Behrends Kraemer, Filipe Morrás, Héctor J. M. Aguilar, Orlando Mario |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Bioquímica Cyanobacteria and Geobacter nifH transcripts soil diazotrophic community soil management practices |
topic |
Bioquímica Cyanobacteria and Geobacter nifH transcripts soil diazotrophic community soil management practices |
dc.description.none.fl_txt_mv |
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N<sub>2</sub> fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified. Instituto de Biotecnologia y Biologia Molecular |
description |
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N<sub>2</sub> fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/87448 |
url |
http://sedici.unlp.edu.ar/handle/10915/87448 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/2045-8827 info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.502 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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