Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agric...

Autores
Calderoli, Priscila Anabel; Collavino, Mónica Mariana; Behrends Kraemer, Filipe; Morrás, Héctor J. M.; Aguilar, Orlando Mario
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
Instituto de Biotecnologia y Biologia Molecular
Materia
Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87448

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network_name_str SEDICI (UNLP)
spelling Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soilCalderoli, Priscila AnabelCollavino, Mónica MarianaBehrends Kraemer, FilipeMorrás, Héctor J. M.Aguilar, Orlando MarioBioquímicaCyanobacteria and GeobacternifH transcriptssoil diazotrophic communitysoil management practicesIn this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N<sub>2</sub> fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.Instituto de Biotecnologia y Biologia Molecular2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87448enginfo:eu-repo/semantics/altIdentifier/issn/2045-8827info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.502info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:10Zoai:sedici.unlp.edu.ar:10915/87448Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:10.353SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
spellingShingle Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
Calderoli, Priscila Anabel
Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
title_short Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_full Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_fullStr Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_full_unstemmed Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
title_sort Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N<sub>2</sub>-fixing community depending on depth and agricultural use of soil
dc.creator.none.fl_str_mv Calderoli, Priscila Anabel
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Orlando Mario
author Calderoli, Priscila Anabel
author_facet Calderoli, Priscila Anabel
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Orlando Mario
author_role author
author2 Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Orlando Mario
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
topic Bioquímica
Cyanobacteria and Geobacter
nifH transcripts
soil diazotrophic community
soil management practices
dc.description.none.fl_txt_mv In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N<sub>2</sub> fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
Instituto de Biotecnologia y Biologia Molecular
description In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N<sub>2</sub> fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/87448
url http://sedici.unlp.edu.ar/handle/10915/87448
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2045-8827
info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.502
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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