Effect of different carbon sources on Δ5 desaturation activity of HTC cells

Autores
Tacconi de Alaniz, María Josefa; Tacconi de Gómez Dumm, Irma Nelva; Brenner, Rodolfo Roberto
Año de publicación
1982
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of different carbon sources on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted. cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on Δ5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar Δ5 desaturation activity to those cells incubated in S-77 medium alone.
Facultad de Ciencias Médicas
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/141342
Acceder
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network_name_str SEDICI (UNLP)
spelling Effect of different carbon sources on Δ5 desaturation activity of HTC cellsTacconi de Alaniz, María JosefaTacconi de Gómez Dumm, Irma NelvaBrenner, Rodolfo RobertoCiencias ExactasΔ5 desaturationMinimal Deviation Hepatoma 7288 cFisiologíaThe effect of different carbon sources on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted. cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on Δ5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar Δ5 desaturation activity to those cells incubated in S-77 medium alone.Facultad de Ciencias MédicasFacultad de Ciencias Exactas1982-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf15-18http://sedici.unlp.edu.ar/handle/10915/141342enginfo:eu-repo/semantics/altIdentifier/issn/0300-8177info:eu-repo/semantics/altIdentifier/issn/1573-4919info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00214817info:eu-repo/semantics/altIdentifier/pmid/6817076info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:11Zoai:sedici.unlp.edu.ar:10915/141342Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:12.085SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title Effect of different carbon sources on Δ5 desaturation activity of HTC cells
spellingShingle Effect of different carbon sources on Δ5 desaturation activity of HTC cells
Tacconi de Alaniz, María Josefa
Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
title_short Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_full Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_fullStr Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_full_unstemmed Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_sort Effect of different carbon sources on Δ5 desaturation activity of HTC cells
dc.creator.none.fl_str_mv Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author Tacconi de Alaniz, María Josefa
author_facet Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author_role author
author2 Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
topic Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
dc.description.none.fl_txt_mv The effect of different carbon sources on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted. cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on Δ5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar Δ5 desaturation activity to those cells incubated in S-77 medium alone.
Facultad de Ciencias Médicas
Facultad de Ciencias Exactas
description The effect of different carbon sources on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted. cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on Δ5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar Δ5 desaturation activity to those cells incubated in S-77 medium alone.
publishDate 1982
dc.date.none.fl_str_mv 1982-10-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/141342
url http://sedici.unlp.edu.ar/handle/10915/141342
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0300-8177
info:eu-repo/semantics/altIdentifier/issn/1573-4919
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00214817
info:eu-repo/semantics/altIdentifier/pmid/6817076
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
15-18
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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