The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells

Autores
Tacconi de Alaniz, María Josefa; Tacconi de Gómez Dumm, Irma Nelva; Brenner, Rodolfo Roberto
Año de publicación
1976
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Incubation of HTC cells (7288 C) with 114C-α-linolenic acid in Swim's 77 medium during 24 hours converted the fatty acid to octadeca-6,9,12,15-tetraenoic acid, eicosa-11,14,17-trienoic acid, eicosa-8,11,14,17-tetraenoic acid, eicosa-5,11,14,17-tetraenoic acid, eicosa-5,8,11,14,17-pentaenoic acid and unsaturated acids of 22 carbons. The existence of two pathways was recognized: one initiated by a Δ6-desaturation and the other by an elongation ofα-linolenic acid. Incubation of the cells with insulin and dibutyryl cyclic AMP modified both pathways in different ways. HTC cells were sensitive to insulin which enhanced the de-saturating route increasing eicosapentaenoic acid synthesis and depressed the elongating route decreasing eicosatrienoic acid. In an opposite way, dibutyryl cyclic AMP decreased eicosapentaenoic acid synthesis and increased eicosatrienoic acid.
Facultad de Ciencias Médicas
Materia
Medicina
Bioquímica
HTC cells
a-linolenic acid
A6-desaturation
insulin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140246
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/140246
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cellsTacconi de Alaniz, María JosefaTacconi de Gómez Dumm, Irma NelvaBrenner, Rodolfo RobertoMedicinaBioquímicaHTC cellsa-linolenic acidA6-desaturationinsulinIncubation of HTC cells (7288 C) with 114C-α-linolenic acid in Swim's 77 medium during 24 hours converted the fatty acid to octadeca-6,9,12,15-tetraenoic acid, eicosa-11,14,17-trienoic acid, eicosa-8,11,14,17-tetraenoic acid, eicosa-5,11,14,17-tetraenoic acid, eicosa-5,8,11,14,17-pentaenoic acid and unsaturated acids of 22 carbons. The existence of two pathways was recognized: one initiated by a Δ6-desaturation and the other by an elongation ofα-linolenic acid. Incubation of the cells with insulin and dibutyryl cyclic AMP modified both pathways in different ways. HTC cells were sensitive to insulin which enhanced the de-saturating route increasing eicosapentaenoic acid synthesis and depressed the elongating route decreasing eicosatrienoic acid. In an opposite way, dibutyryl cyclic AMP decreased eicosapentaenoic acid synthesis and increased eicosatrienoic acid.Facultad de Ciencias Médicas1976-07-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf3-8http://sedici.unlp.edu.ar/handle/10915/140246enginfo:eu-repo/semantics/altIdentifier/issn/0300-8177info:eu-repo/semantics/altIdentifier/issn/1573-4919info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01731897info:eu-repo/semantics/altIdentifier/pmid/184376info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:02Zoai:sedici.unlp.edu.ar:10915/140246Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:03.187SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
title The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
spellingShingle The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
Tacconi de Alaniz, María Josefa
Medicina
Bioquímica
HTC cells
a-linolenic acid
A6-desaturation
insulin
title_short The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
title_full The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
title_fullStr The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
title_full_unstemmed The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
title_sort The action of insulin and dibutyryl cyclic AMP on the biosynthesis of polyunsaturated acids ofα-linolenic acid family in HTC cells
dc.creator.none.fl_str_mv Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author Tacconi de Alaniz, María Josefa
author_facet Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author_role author
author2 Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author2_role author
author
dc.subject.none.fl_str_mv Medicina
Bioquímica
HTC cells
a-linolenic acid
A6-desaturation
insulin
topic Medicina
Bioquímica
HTC cells
a-linolenic acid
A6-desaturation
insulin
dc.description.none.fl_txt_mv Incubation of HTC cells (7288 C) with 114C-α-linolenic acid in Swim's 77 medium during 24 hours converted the fatty acid to octadeca-6,9,12,15-tetraenoic acid, eicosa-11,14,17-trienoic acid, eicosa-8,11,14,17-tetraenoic acid, eicosa-5,11,14,17-tetraenoic acid, eicosa-5,8,11,14,17-pentaenoic acid and unsaturated acids of 22 carbons. The existence of two pathways was recognized: one initiated by a Δ6-desaturation and the other by an elongation ofα-linolenic acid. Incubation of the cells with insulin and dibutyryl cyclic AMP modified both pathways in different ways. HTC cells were sensitive to insulin which enhanced the de-saturating route increasing eicosapentaenoic acid synthesis and depressed the elongating route decreasing eicosatrienoic acid. In an opposite way, dibutyryl cyclic AMP decreased eicosapentaenoic acid synthesis and increased eicosatrienoic acid.
Facultad de Ciencias Médicas
description Incubation of HTC cells (7288 C) with 114C-α-linolenic acid in Swim's 77 medium during 24 hours converted the fatty acid to octadeca-6,9,12,15-tetraenoic acid, eicosa-11,14,17-trienoic acid, eicosa-8,11,14,17-tetraenoic acid, eicosa-5,11,14,17-tetraenoic acid, eicosa-5,8,11,14,17-pentaenoic acid and unsaturated acids of 22 carbons. The existence of two pathways was recognized: one initiated by a Δ6-desaturation and the other by an elongation ofα-linolenic acid. Incubation of the cells with insulin and dibutyryl cyclic AMP modified both pathways in different ways. HTC cells were sensitive to insulin which enhanced the de-saturating route increasing eicosapentaenoic acid synthesis and depressed the elongating route decreasing eicosatrienoic acid. In an opposite way, dibutyryl cyclic AMP decreased eicosapentaenoic acid synthesis and increased eicosatrienoic acid.
publishDate 1976
dc.date.none.fl_str_mv 1976-07-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140246
url http://sedici.unlp.edu.ar/handle/10915/140246
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0300-8177
info:eu-repo/semantics/altIdentifier/issn/1573-4919
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01731897
info:eu-repo/semantics/altIdentifier/pmid/184376
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
3-8
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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