Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells
- Autores
- Tacconi de Alaniz, María Josefa; Tacconi de Gómez Dumm, Irma Nelva; Brenner, Rodolfo Roberto
- Año de publicación
- 1980
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The effect of fasting on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288c (HTC) cells and in rat liver microsomes was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C] eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in complete medium (fed cells). This effect was also shown in rat liver microsomes of rats fasted for 12, 24 and 48 hr. Fasting produced a decrease of the amount of ethanolamine fraction in HTC cells together with a significant increase in the specific activity of the same fraction. Fasting also modified the fatty acid composition of HTC cells and rat liver microsomes. There was observed a relative increase of 18:2ω6 acid. The importance of the difference in fatty acid and lipid composition among fasted and fed HTC cells and rat liver microsomes on Δ5 desaturation activity was discussed.
Facultad de Ciencias Médicas
Consejo Nacional de Investigaciones Científicas y Técnicas - Materia
- Ciencias Médicas
- Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/143730
Ver los metadatos del registro completo
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Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cellsTacconi de Alaniz, María JosefaTacconi de Gómez Dumm, Irma NelvaBrenner, Rodolfo RobertoCiencias MédicasThe effect of fasting on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288c (HTC) cells and in rat liver microsomes was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C] eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in complete medium (fed cells). This effect was also shown in rat liver microsomes of rats fasted for 12, 24 and 48 hr. Fasting produced a decrease of the amount of ethanolamine fraction in HTC cells together with a significant increase in the specific activity of the same fraction. Fasting also modified the fatty acid composition of HTC cells and rat liver microsomes. There was observed a relative increase of 18:2ω6 acid. The importance of the difference in fatty acid and lipid composition among fasted and fed HTC cells and rat liver microsomes on Δ5 desaturation activity was discussed.Facultad de Ciencias MédicasConsejo Nacional de Investigaciones Científicas y Técnicas1980-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf165-170http://sedici.unlp.edu.ar/handle/10915/143730enginfo:eu-repo/semantics/altIdentifier/issn/0300-8177info:eu-repo/semantics/altIdentifier/issn/1573-4919info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00225290info:eu-repo/semantics/altIdentifier/pmid/7464829info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:17Zoai:sedici.unlp.edu.ar:10915/143730Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:18.035SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells |
| title |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells |
| spellingShingle |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells Tacconi de Alaniz, María Josefa Ciencias Médicas |
| title_short |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells |
| title_full |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells |
| title_fullStr |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells |
| title_full_unstemmed |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells |
| title_sort |
Effect of fasting on Δ5 desaturation activity in rat liver microsomes and HTC cells |
| dc.creator.none.fl_str_mv |
Tacconi de Alaniz, María Josefa Tacconi de Gómez Dumm, Irma Nelva Brenner, Rodolfo Roberto |
| author |
Tacconi de Alaniz, María Josefa |
| author_facet |
Tacconi de Alaniz, María Josefa Tacconi de Gómez Dumm, Irma Nelva Brenner, Rodolfo Roberto |
| author_role |
author |
| author2 |
Tacconi de Gómez Dumm, Irma Nelva Brenner, Rodolfo Roberto |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Ciencias Médicas |
| topic |
Ciencias Médicas |
| dc.description.none.fl_txt_mv |
The effect of fasting on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288c (HTC) cells and in rat liver microsomes was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C] eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in complete medium (fed cells). This effect was also shown in rat liver microsomes of rats fasted for 12, 24 and 48 hr. Fasting produced a decrease of the amount of ethanolamine fraction in HTC cells together with a significant increase in the specific activity of the same fraction. Fasting also modified the fatty acid composition of HTC cells and rat liver microsomes. There was observed a relative increase of 18:2ω6 acid. The importance of the difference in fatty acid and lipid composition among fasted and fed HTC cells and rat liver microsomes on Δ5 desaturation activity was discussed. Facultad de Ciencias Médicas Consejo Nacional de Investigaciones Científicas y Técnicas |
| description |
The effect of fasting on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288c (HTC) cells and in rat liver microsomes was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C] eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in complete medium (fed cells). This effect was also shown in rat liver microsomes of rats fasted for 12, 24 and 48 hr. Fasting produced a decrease of the amount of ethanolamine fraction in HTC cells together with a significant increase in the specific activity of the same fraction. Fasting also modified the fatty acid composition of HTC cells and rat liver microsomes. There was observed a relative increase of 18:2ω6 acid. The importance of the difference in fatty acid and lipid composition among fasted and fed HTC cells and rat liver microsomes on Δ5 desaturation activity was discussed. |
| publishDate |
1980 |
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1980-12-16 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/143730 |
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eng |
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eng |
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