nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics

Autores
Collavino, Mónica Mariana; Tripp, H. James; Frank, Ildiko E.; Vidoz, María Laura; Calderoli, Priscila Anabel; Donato, Mariano Humberto; Zehr, Jonathan P.; Aguilar, Orlando Mario
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A dataset of 87020 nifH reads and 16782 unique nifH protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N2-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of Alphaproteobacteria, Betaproteobacteria and Deltaproteobacteria, mainly relatives to Bradyrhizobium and Methylosinus/Methylocystis, but a surprising paucity of Gammaproteobacteria. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on nifH diversity. nifH gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N2-fixing bacteria.
Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Tripp, H. James. University of California; Estados Unidos
Fil: Frank, Ildiko E.. University of California; Estados Unidos
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Calderoli, Priscila Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Donato, Mariano Humberto. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Sistemática y Biología Evolutiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zehr, Jonathan P.. University of California; Estados Unidos
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Community
Composition
Diversity
Nifh
Soil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3399
Acceder
id CONICETDig_3c7e076de1ed26afbc6638b3cc559848
oai_identifier_str oai:ri.conicet.gov.ar:11336/3399
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamicsCollavino, Mónica MarianaTripp, H. JamesFrank, Ildiko E.Vidoz, María LauraCalderoli, Priscila AnabelDonato, Mariano HumbertoZehr, Jonathan P.Aguilar, Orlando MarioCommunityCompositionDiversityNifhSoilhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A dataset of 87020 nifH reads and 16782 unique nifH protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N2-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of Alphaproteobacteria, Betaproteobacteria and Deltaproteobacteria, mainly relatives to Bradyrhizobium and Methylosinus/Methylocystis, but a surprising paucity of Gammaproteobacteria. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on nifH diversity. nifH gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N2-fixing bacteria.Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Tripp, H. James. University of California; Estados UnidosFil: Frank, Ildiko E.. University of California; Estados UnidosFil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Calderoli, Priscila Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Donato, Mariano Humberto. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Sistemática y Biología Evolutiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zehr, Jonathan P.. University of California; Estados UnidosFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaWiley Blackwell Publishing, Inc2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3399Collavino, Mónica Mariana; Tripp, H. James; Frank, Ildiko E.; Vidoz, María Laura; Calderoli, Priscila Anabel; et al.; nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 16; 10; 10-2014; 3211-32231462-2912enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12423/abstractinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/1462-2920.12423info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:58Zoai:ri.conicet.gov.ar:11336/3399instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:59.082CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
title nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
spellingShingle nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
Collavino, Mónica Mariana
Community
Composition
Diversity
Nifh
Soil
title_short nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
title_full nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
title_fullStr nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
title_full_unstemmed nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
title_sort nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics
dc.creator.none.fl_str_mv Collavino, Mónica Mariana
Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author Collavino, Mónica Mariana
author_facet Collavino, Mónica Mariana
Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author_role author
author2 Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Community
Composition
Diversity
Nifh
Soil
topic Community
Composition
Diversity
Nifh
Soil
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A dataset of 87020 nifH reads and 16782 unique nifH protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N2-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of Alphaproteobacteria, Betaproteobacteria and Deltaproteobacteria, mainly relatives to Bradyrhizobium and Methylosinus/Methylocystis, but a surprising paucity of Gammaproteobacteria. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on nifH diversity. nifH gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N2-fixing bacteria.
Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Tripp, H. James. University of California; Estados Unidos
Fil: Frank, Ildiko E.. University of California; Estados Unidos
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Calderoli, Priscila Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Donato, Mariano Humberto. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Sistemática y Biología Evolutiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zehr, Jonathan P.. University of California; Estados Unidos
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
description A dataset of 87020 nifH reads and 16782 unique nifH protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N2-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of Alphaproteobacteria, Betaproteobacteria and Deltaproteobacteria, mainly relatives to Bradyrhizobium and Methylosinus/Methylocystis, but a surprising paucity of Gammaproteobacteria. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on nifH diversity. nifH gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N2-fixing bacteria.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3399
Collavino, Mónica Mariana; Tripp, H. James; Frank, Ildiko E.; Vidoz, María Laura; Calderoli, Priscila Anabel; et al.; nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 16; 10; 10-2014; 3211-3223
1462-2912
url http://hdl.handle.net/11336/3399
identifier_str_mv Collavino, Mónica Mariana; Tripp, H. James; Frank, Ildiko E.; Vidoz, María Laura; Calderoli, Priscila Anabel; et al.; nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 16; 10; 10-2014; 3211-3223
1462-2912
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12423/abstract
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/1462-2920.12423
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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