Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis

Autores
Hartleben, Cláudia P.; Leal, Fernanda M. A.; Monte, Leonardo G.; Hartwig, Daiane D.; Seixas, Fabiana K.; Vasconcellos, Sílvio A.; Brihuega, Bibiana Felicitas; Dellagostin, Odir A.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.
Instituto de Patobiología
Fil: Hartleben, Cláudia P. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Leal, Fernanda M. A. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Monte, Leonardo G. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Hartwig, Daiane D. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil
Fil: Seixas, Fabiana K. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratório de Genômica Funcional; Brasil
Fil: Vasconcellos, Sílvio A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil
Fuente
Current Microbiology 66 (2) : 106–109 (February 2013)
Materia
Enfermedades de los Animales
Leptospirosis
Cerdo
Técnicas Inmunológicas
Antígenos
ELISA
Animal Diseases
Swine
Immunological Techniques
Antigens
Análisis Serológico
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4273
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4273
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine LeptospirosisHartleben, Cláudia P.Leal, Fernanda M. A.Monte, Leonardo G.Hartwig, Daiane D.Seixas, Fabiana K.Vasconcellos, Sílvio A.Brihuega, Bibiana FelicitasDellagostin, Odir A.Enfermedades de los AnimalesLeptospirosisCerdoTécnicas InmunológicasAntígenosELISAAnimal DiseasesSwineImmunological TechniquesAntigensAnálisis SerológicoLeptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.Instituto de PatobiologíaFil: Hartleben, Cláudia P. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; BrasilFil: Leal, Fernanda M. A. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; BrasilFil: Monte, Leonardo G. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; BrasilFil: Hartwig, Daiane D. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; BrasilFil: Seixas, Fabiana K. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratório de Genômica Funcional; BrasilFil: Vasconcellos, Sílvio A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; BrasilFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; BrasilSpringer2019-01-16T13:59:11Z2019-01-16T13:59:11Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007%2Fs00284-012-0237-xhttp://hdl.handle.net/20.500.12123/42730343-86511432-0991https://doi.org/10.1007/s00284-012-0237-xCurrent Microbiology 66 (2) : 106–109 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:25Zoai:localhost:20.500.12123/4273instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:25.521INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
title Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
spellingShingle Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
Hartleben, Cláudia P.
Enfermedades de los Animales
Leptospirosis
Cerdo
Técnicas Inmunológicas
Antígenos
ELISA
Animal Diseases
Swine
Immunological Techniques
Antigens
Análisis Serológico
title_short Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
title_full Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
title_fullStr Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
title_full_unstemmed Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
title_sort Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
dc.creator.none.fl_str_mv Hartleben, Cláudia P.
Leal, Fernanda M. A.
Monte, Leonardo G.
Hartwig, Daiane D.
Seixas, Fabiana K.
Vasconcellos, Sílvio A.
Brihuega, Bibiana Felicitas
Dellagostin, Odir A.
author Hartleben, Cláudia P.
author_facet Hartleben, Cláudia P.
Leal, Fernanda M. A.
Monte, Leonardo G.
Hartwig, Daiane D.
Seixas, Fabiana K.
Vasconcellos, Sílvio A.
Brihuega, Bibiana Felicitas
Dellagostin, Odir A.
author_role author
author2 Leal, Fernanda M. A.
Monte, Leonardo G.
Hartwig, Daiane D.
Seixas, Fabiana K.
Vasconcellos, Sílvio A.
Brihuega, Bibiana Felicitas
Dellagostin, Odir A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Leptospirosis
Cerdo
Técnicas Inmunológicas
Antígenos
ELISA
Animal Diseases
Swine
Immunological Techniques
Antigens
Análisis Serológico
topic Enfermedades de los Animales
Leptospirosis
Cerdo
Técnicas Inmunológicas
Antígenos
ELISA
Animal Diseases
Swine
Immunological Techniques
Antigens
Análisis Serológico
dc.description.none.fl_txt_mv Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.
Instituto de Patobiología
Fil: Hartleben, Cláudia P. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Leal, Fernanda M. A. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Monte, Leonardo G. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Hartwig, Daiane D. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil
Fil: Seixas, Fabiana K. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratório de Genômica Funcional; Brasil
Fil: Vasconcellos, Sílvio A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil
description Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
2019-01-16T13:59:11Z
2019-01-16T13:59:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007%2Fs00284-012-0237-x
http://hdl.handle.net/20.500.12123/4273
0343-8651
1432-0991
https://doi.org/10.1007/s00284-012-0237-x
url https://link.springer.com/article/10.1007%2Fs00284-012-0237-x
http://hdl.handle.net/20.500.12123/4273
https://doi.org/10.1007/s00284-012-0237-x
identifier_str_mv 0343-8651
1432-0991
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Current Microbiology 66 (2) : 106–109 (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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