Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis
- Autores
- Hartleben, Cláudia P.; Leal, Fernanda M. A.; Monte, Leonardo G.; Hartwig, Daiane D.; Seixas, Fabiana K.; Vasconcellos, Sílvio A.; Brihuega, Bibiana Felicitas; Dellagostin, Odir A.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.
Instituto de Patobiología
Fil: Hartleben, Cláudia P. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Leal, Fernanda M. A. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Monte, Leonardo G. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil
Fil: Hartwig, Daiane D. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil
Fil: Seixas, Fabiana K. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratório de Genômica Funcional; Brasil
Fil: Vasconcellos, Sílvio A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil - Fuente
- Current Microbiology 66 (2) : 106–109 (February 2013)
- Materia
-
Enfermedades de los Animales
Leptospirosis
Cerdo
Técnicas Inmunológicas
Antígenos
ELISA
Animal Diseases
Swine
Immunological Techniques
Antigens
Análisis Serológico - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4273
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Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine LeptospirosisHartleben, Cláudia P.Leal, Fernanda M. A.Monte, Leonardo G.Hartwig, Daiane D.Seixas, Fabiana K.Vasconcellos, Sílvio A.Brihuega, Bibiana FelicitasDellagostin, Odir A.Enfermedades de los AnimalesLeptospirosisCerdoTécnicas InmunológicasAntígenosELISAAnimal DiseasesSwineImmunological TechniquesAntigensAnálisis SerológicoLeptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.Instituto de PatobiologíaFil: Hartleben, Cláudia P. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; BrasilFil: Leal, Fernanda M. A. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; BrasilFil: Monte, Leonardo G. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; BrasilFil: Hartwig, Daiane D. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; BrasilFil: Seixas, Fabiana K. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratório de Genômica Funcional; BrasilFil: Vasconcellos, Sílvio A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; BrasilFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; BrasilSpringer2019-01-16T13:59:11Z2019-01-16T13:59:11Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007%2Fs00284-012-0237-xhttp://hdl.handle.net/20.500.12123/42730343-86511432-0991https://doi.org/10.1007/s00284-012-0237-xCurrent Microbiology 66 (2) : 106–109 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4273instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:33.446INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis |
title |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis |
spellingShingle |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis Hartleben, Cláudia P. Enfermedades de los Animales Leptospirosis Cerdo Técnicas Inmunológicas Antígenos ELISA Animal Diseases Swine Immunological Techniques Antigens Análisis Serológico |
title_short |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis |
title_full |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis |
title_fullStr |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis |
title_full_unstemmed |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis |
title_sort |
Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis |
dc.creator.none.fl_str_mv |
Hartleben, Cláudia P. Leal, Fernanda M. A. Monte, Leonardo G. Hartwig, Daiane D. Seixas, Fabiana K. Vasconcellos, Sílvio A. Brihuega, Bibiana Felicitas Dellagostin, Odir A. |
author |
Hartleben, Cláudia P. |
author_facet |
Hartleben, Cláudia P. Leal, Fernanda M. A. Monte, Leonardo G. Hartwig, Daiane D. Seixas, Fabiana K. Vasconcellos, Sílvio A. Brihuega, Bibiana Felicitas Dellagostin, Odir A. |
author_role |
author |
author2 |
Leal, Fernanda M. A. Monte, Leonardo G. Hartwig, Daiane D. Seixas, Fabiana K. Vasconcellos, Sílvio A. Brihuega, Bibiana Felicitas Dellagostin, Odir A. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Enfermedades de los Animales Leptospirosis Cerdo Técnicas Inmunológicas Antígenos ELISA Animal Diseases Swine Immunological Techniques Antigens Análisis Serológico |
topic |
Enfermedades de los Animales Leptospirosis Cerdo Técnicas Inmunológicas Antígenos ELISA Animal Diseases Swine Immunological Techniques Antigens Análisis Serológico |
dc.description.none.fl_txt_mv |
Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys. Instituto de Patobiología Fil: Hartleben, Cláudia P. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil Fil: Leal, Fernanda M. A. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil Fil: Monte, Leonardo G. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasil Fil: Hartwig, Daiane D. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil Fil: Seixas, Fabiana K. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratório de Genômica Funcional; Brasil Fil: Vasconcellos, Sílvio A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasil |
description |
Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02 2019-01-16T13:59:11Z 2019-01-16T13:59:11Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://link.springer.com/article/10.1007%2Fs00284-012-0237-x http://hdl.handle.net/20.500.12123/4273 0343-8651 1432-0991 https://doi.org/10.1007/s00284-012-0237-x |
url |
https://link.springer.com/article/10.1007%2Fs00284-012-0237-x http://hdl.handle.net/20.500.12123/4273 https://doi.org/10.1007/s00284-012-0237-x |
identifier_str_mv |
0343-8651 1432-0991 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
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restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Current Microbiology 66 (2) : 106–109 (February 2013) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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