Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis

Autores
Martinez, Mara Leila; Rodriguez, Marcelo A.; Saraullo, Vanina Rosa; Irazu, Lucía E.; Hamer, Micaela; Watanabe, Olivia; Grune Loffler, Sylvia; Romero, Graciela Noemi; Samartino, Luis Ernesto; Brihuega, Bibiana Felicitas
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Leptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 with sonicated antigen for detecting anti-Leptospira IgG antibodies in bovine serum using ELISA. The Microscopic Agglutination Test (MAT) is used as the gold standard. Sonicated antigen from cultures of Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain M20) was used for the eELISA and rLipL32 for the rELISA. The performance of these assays was evaluated using serum samples from 166 bovines, 69 MAT positive and 97 MAT negative. At the optimal cut-off point recommended by the receiver operating characteristic (ROC) curve analysis, the sensitivity and specificity values were 98.6% and 97.9%, respectively, for eELISA, and 85.5% and 86.6% respectively, for rELISA. The value for the area under the ROC curve was 0.998 (0.994-1.0) (CI 95%) for eELISA and 0.929 (0.891-0.968) (CI 95%) for rELISA. The ROC curves for rLipL32 and sonicated antigen showed statistically significant differences (z = -3.826; p = 0.000). A three-way comparison showed statistically significant differences in the sensitivity and specificity of rELISA and eELISA. Our results showed that eELISA was more specific and sensitive than rELISA. The difference in performance (eELISA-rELISA) was 13.4% (4.03-23.28) (CI 95%) for sensitivity and 11.34 % (4.07-19.56) (CI 95%) for specificity. Our results show that the eELISA has a better diagnostic performance than rELISA for the detection of anti-Leptospira IgG antibodies in bovine serum.
Instituto de Patobiología
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Acta Tropica Volume 225 : 106214 (January 2022)
Materia
Ganado Bovino
Enfermedades de los Animales
ELISA
Antígenos
Cattle
Animal Diseases
Leptospira
Leptospirosis
Antigens
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10741
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10741
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosisMartinez, Mara LeilaRodriguez, Marcelo A.Saraullo, Vanina RosaIrazu, Lucía E.Hamer, MicaelaWatanabe, OliviaGrune Loffler, SylviaRomero, Graciela NoemiSamartino, Luis ErnestoBrihuega, Bibiana FelicitasGanado BovinoEnfermedades de los AnimalesELISAAntígenosCattleAnimal DiseasesLeptospiraLeptospirosisAntigensLeptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 with sonicated antigen for detecting anti-Leptospira IgG antibodies in bovine serum using ELISA. The Microscopic Agglutination Test (MAT) is used as the gold standard. Sonicated antigen from cultures of Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain M20) was used for the eELISA and rLipL32 for the rELISA. The performance of these assays was evaluated using serum samples from 166 bovines, 69 MAT positive and 97 MAT negative. At the optimal cut-off point recommended by the receiver operating characteristic (ROC) curve analysis, the sensitivity and specificity values were 98.6% and 97.9%, respectively, for eELISA, and 85.5% and 86.6% respectively, for rELISA. The value for the area under the ROC curve was 0.998 (0.994-1.0) (CI 95%) for eELISA and 0.929 (0.891-0.968) (CI 95%) for rELISA. The ROC curves for rLipL32 and sonicated antigen showed statistically significant differences (z = -3.826; p = 0.000). A three-way comparison showed statistically significant differences in the sensitivity and specificity of rELISA and eELISA. Our results showed that eELISA was more specific and sensitive than rELISA. The difference in performance (eELISA-rELISA) was 13.4% (4.03-23.28) (CI 95%) for sensitivity and 11.34 % (4.07-19.56) (CI 95%) for specificity. Our results show that the eELISA has a better diagnostic performance than rELISA for the detection of anti-Leptospira IgG antibodies in bovine serum.Instituto de PatobiologíaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2022-11-102021-11-10T17:43:43Z2021-11-10T17:43:43Z2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10741https://www.sciencedirect.com/science/article/abs/pii/S0001706X210039220001-706Xhttps://doi.org/10.1016/j.actatropica.2021.106214Acta Tropica Volume 225 : 106214 (January 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.info:eu-repo/semantics/openAccess2025-10-16T09:30:17Zoai:localhost:20.500.12123/10741instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:17.935INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
title Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
spellingShingle Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
Martinez, Mara Leila
Ganado Bovino
Enfermedades de los Animales
ELISA
Antígenos
Cattle
Animal Diseases
Leptospira
Leptospirosis
Antigens
title_short Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
title_full Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
title_fullStr Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
title_full_unstemmed Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
title_sort Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
dc.creator.none.fl_str_mv Martinez, Mara Leila
Rodriguez, Marcelo A.
Saraullo, Vanina Rosa
Irazu, Lucía E.
Hamer, Micaela
Watanabe, Olivia
Grune Loffler, Sylvia
Romero, Graciela Noemi
Samartino, Luis Ernesto
Brihuega, Bibiana Felicitas
author Martinez, Mara Leila
author_facet Martinez, Mara Leila
Rodriguez, Marcelo A.
Saraullo, Vanina Rosa
Irazu, Lucía E.
Hamer, Micaela
Watanabe, Olivia
Grune Loffler, Sylvia
Romero, Graciela Noemi
Samartino, Luis Ernesto
Brihuega, Bibiana Felicitas
author_role author
author2 Rodriguez, Marcelo A.
Saraullo, Vanina Rosa
Irazu, Lucía E.
Hamer, Micaela
Watanabe, Olivia
Grune Loffler, Sylvia
Romero, Graciela Noemi
Samartino, Luis Ernesto
Brihuega, Bibiana Felicitas
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
ELISA
Antígenos
Cattle
Animal Diseases
Leptospira
Leptospirosis
Antigens
topic Ganado Bovino
Enfermedades de los Animales
ELISA
Antígenos
Cattle
Animal Diseases
Leptospira
Leptospirosis
Antigens
dc.description.none.fl_txt_mv Leptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 with sonicated antigen for detecting anti-Leptospira IgG antibodies in bovine serum using ELISA. The Microscopic Agglutination Test (MAT) is used as the gold standard. Sonicated antigen from cultures of Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain M20) was used for the eELISA and rLipL32 for the rELISA. The performance of these assays was evaluated using serum samples from 166 bovines, 69 MAT positive and 97 MAT negative. At the optimal cut-off point recommended by the receiver operating characteristic (ROC) curve analysis, the sensitivity and specificity values were 98.6% and 97.9%, respectively, for eELISA, and 85.5% and 86.6% respectively, for rELISA. The value for the area under the ROC curve was 0.998 (0.994-1.0) (CI 95%) for eELISA and 0.929 (0.891-0.968) (CI 95%) for rELISA. The ROC curves for rLipL32 and sonicated antigen showed statistically significant differences (z = -3.826; p = 0.000). A three-way comparison showed statistically significant differences in the sensitivity and specificity of rELISA and eELISA. Our results showed that eELISA was more specific and sensitive than rELISA. The difference in performance (eELISA-rELISA) was 13.4% (4.03-23.28) (CI 95%) for sensitivity and 11.34 % (4.07-19.56) (CI 95%) for specificity. Our results show that the eELISA has a better diagnostic performance than rELISA for the detection of anti-Leptospira IgG antibodies in bovine serum.
Instituto de Patobiología
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Leptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 with sonicated antigen for detecting anti-Leptospira IgG antibodies in bovine serum using ELISA. The Microscopic Agglutination Test (MAT) is used as the gold standard. Sonicated antigen from cultures of Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain M20) was used for the eELISA and rLipL32 for the rELISA. The performance of these assays was evaluated using serum samples from 166 bovines, 69 MAT positive and 97 MAT negative. At the optimal cut-off point recommended by the receiver operating characteristic (ROC) curve analysis, the sensitivity and specificity values were 98.6% and 97.9%, respectively, for eELISA, and 85.5% and 86.6% respectively, for rELISA. The value for the area under the ROC curve was 0.998 (0.994-1.0) (CI 95%) for eELISA and 0.929 (0.891-0.968) (CI 95%) for rELISA. The ROC curves for rLipL32 and sonicated antigen showed statistically significant differences (z = -3.826; p = 0.000). A three-way comparison showed statistically significant differences in the sensitivity and specificity of rELISA and eELISA. Our results showed that eELISA was more specific and sensitive than rELISA. The difference in performance (eELISA-rELISA) was 13.4% (4.03-23.28) (CI 95%) for sensitivity and 11.34 % (4.07-19.56) (CI 95%) for specificity. Our results show that the eELISA has a better diagnostic performance than rELISA for the detection of anti-Leptospira IgG antibodies in bovine serum.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-10T17:43:43Z
2021-11-10T17:43:43Z
2022-01
info:eu-repo/date/embargoEnd/2022-11-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10741
https://www.sciencedirect.com/science/article/abs/pii/S0001706X21003922
0001-706X
https://doi.org/10.1016/j.actatropica.2021.106214
url http://hdl.handle.net/20.500.12123/10741
https://www.sciencedirect.com/science/article/abs/pii/S0001706X21003922
https://doi.org/10.1016/j.actatropica.2021.106214
identifier_str_mv 0001-706X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Acta Tropica Volume 225 : 106214 (January 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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