Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers

Autores
Robles, Carlos Alejandro; Nielsen, Klaus; Gall, David; Willems, Priscila Mabel
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was used. The animals were bled on day 0 and then subcutaneously vaccinated with 2 ml of a commercially available SRB51 vaccine (Schering-PloughTM) containing 1 107 to 3.4 107 viable cells. Blood samples without anticoagulant for sera obtaining were then collected at days 30, 90, 210 and 360 post-vaccination. To detect the SRB51 antibodies, Brucella ovis hot saline extract, B. ovis RLPS (RLPS), and SRB51-RLPS were used. The buffered antigen plate agglutination test and an indirect enzyme-linked immunoassay (I-ELISA) using the smooth LPS (SLPS) antigen from B. abortus were used as control tests. All the sera samples were negative in the BPA test and in the standard I-ELISA using the SLPS. The SRB51-RLPS and the B. ovis RLPS antigens performed better than the B. ovis hot saline extract antigen.
Estación Experimental Agropecuaria Bariloche
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Gall, David. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Willems, Priscila Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fuente
Veterinary Immunology and Immunopathology 127 : 153-155 (2009)
Materia
Brucella
Brucella abortus
Brucelosis
Antígenos
Anticuerpos
Técnicas Inmunológicas
Ganado Bovino
Brucellosis
Antigens
Antibodies
Immunological Techniques
Cattle
Región Patagónica
Inmunoensayo
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11134
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11134
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network_name_str INTA Digital (INTA)
spelling Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifersRobles, Carlos AlejandroNielsen, KlausGall, DavidWillems, Priscila MabelBrucellaBrucella abortusBrucelosisAntígenosAnticuerposTécnicas InmunológicasGanado BovinoBrucellosisAntigensAntibodiesImmunological TechniquesCattleRegión PatagónicaInmunoensayoThe live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was used. The animals were bled on day 0 and then subcutaneously vaccinated with 2 ml of a commercially available SRB51 vaccine (Schering-PloughTM) containing 1 107 to 3.4 107 viable cells. Blood samples without anticoagulant for sera obtaining were then collected at days 30, 90, 210 and 360 post-vaccination. To detect the SRB51 antibodies, Brucella ovis hot saline extract, B. ovis RLPS (RLPS), and SRB51-RLPS were used. The buffered antigen plate agglutination test and an indirect enzyme-linked immunoassay (I-ELISA) using the smooth LPS (SLPS) antigen from B. abortus were used as control tests. All the sera samples were negative in the BPA test and in the standard I-ELISA using the SLPS. The SRB51-RLPS and the B. ovis RLPS antigens performed better than the B. ovis hot saline extract antigen.Estación Experimental Agropecuaria BarilocheFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaFil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; CanadáFil: Gall, David. Canadian Food Inspection Agency. Ottawa Laboratories; CanadáFil: Willems, Priscila Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaElsevier2022-02-14T11:44:37Z2022-02-14T11:44:37Z2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11134https://www.sciencedirect.com/science/article/abs/pii/S01652427080034860165-2427https://doi.org/10.1016/j.vetimm.2008.09.007Veterinary Immunology and Immunopathology 127 : 153-155 (2009)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:28Zoai:localhost:20.500.12123/11134instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:28.476INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
title Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
spellingShingle Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
Robles, Carlos Alejandro
Brucella
Brucella abortus
Brucelosis
Antígenos
Anticuerpos
Técnicas Inmunológicas
Ganado Bovino
Brucellosis
Antigens
Antibodies
Immunological Techniques
Cattle
Región Patagónica
Inmunoensayo
title_short Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
title_full Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
title_fullStr Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
title_full_unstemmed Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
title_sort Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
dc.creator.none.fl_str_mv Robles, Carlos Alejandro
Nielsen, Klaus
Gall, David
Willems, Priscila Mabel
author Robles, Carlos Alejandro
author_facet Robles, Carlos Alejandro
Nielsen, Klaus
Gall, David
Willems, Priscila Mabel
author_role author
author2 Nielsen, Klaus
Gall, David
Willems, Priscila Mabel
author2_role author
author
author
dc.subject.none.fl_str_mv Brucella
Brucella abortus
Brucelosis
Antígenos
Anticuerpos
Técnicas Inmunológicas
Ganado Bovino
Brucellosis
Antigens
Antibodies
Immunological Techniques
Cattle
Región Patagónica
Inmunoensayo
topic Brucella
Brucella abortus
Brucelosis
Antígenos
Anticuerpos
Técnicas Inmunológicas
Ganado Bovino
Brucellosis
Antigens
Antibodies
Immunological Techniques
Cattle
Región Patagónica
Inmunoensayo
dc.description.none.fl_txt_mv The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was used. The animals were bled on day 0 and then subcutaneously vaccinated with 2 ml of a commercially available SRB51 vaccine (Schering-PloughTM) containing 1 107 to 3.4 107 viable cells. Blood samples without anticoagulant for sera obtaining were then collected at days 30, 90, 210 and 360 post-vaccination. To detect the SRB51 antibodies, Brucella ovis hot saline extract, B. ovis RLPS (RLPS), and SRB51-RLPS were used. The buffered antigen plate agglutination test and an indirect enzyme-linked immunoassay (I-ELISA) using the smooth LPS (SLPS) antigen from B. abortus were used as control tests. All the sera samples were negative in the BPA test and in the standard I-ELISA using the SLPS. The SRB51-RLPS and the B. ovis RLPS antigens performed better than the B. ovis hot saline extract antigen.
Estación Experimental Agropecuaria Bariloche
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Gall, David. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Willems, Priscila Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
description The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was used. The animals were bled on day 0 and then subcutaneously vaccinated with 2 ml of a commercially available SRB51 vaccine (Schering-PloughTM) containing 1 107 to 3.4 107 viable cells. Blood samples without anticoagulant for sera obtaining were then collected at days 30, 90, 210 and 360 post-vaccination. To detect the SRB51 antibodies, Brucella ovis hot saline extract, B. ovis RLPS (RLPS), and SRB51-RLPS were used. The buffered antigen plate agglutination test and an indirect enzyme-linked immunoassay (I-ELISA) using the smooth LPS (SLPS) antigen from B. abortus were used as control tests. All the sera samples were negative in the BPA test and in the standard I-ELISA using the SLPS. The SRB51-RLPS and the B. ovis RLPS antigens performed better than the B. ovis hot saline extract antigen.
publishDate 2009
dc.date.none.fl_str_mv 2009
2022-02-14T11:44:37Z
2022-02-14T11:44:37Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11134
https://www.sciencedirect.com/science/article/abs/pii/S0165242708003486
0165-2427
https://doi.org/10.1016/j.vetimm.2008.09.007
url http://hdl.handle.net/20.500.12123/11134
https://www.sciencedirect.com/science/article/abs/pii/S0165242708003486
https://doi.org/10.1016/j.vetimm.2008.09.007
identifier_str_mv 0165-2427
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 127 : 153-155 (2009)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.891075

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