Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay

Autores
Spotorno, Viviana Graciela; Tezón, Jorge Guillermo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratio
Instituto de Tecnología de Alimentos
Fil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
Fil: Tezon, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fuente
Food and Agricultural Immunology 23 (4) : 315-328 (December 2012)
Materia
Técnicas Inmunológicas
Antígenos
Biotina
Técnicas Inmunoenzimáticas
Clenbuterol
Immunological Techniques
Antigens
Biotin
Immunoenzyme Techniques
Inmunoensayos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3977
Acceder
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spelling Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassaySpotorno, Viviana GracielaTezón, Jorge GuillermoTécnicas InmunológicasAntígenosBiotinaTécnicas InmunoenzimáticasClenbuterolImmunological TechniquesAntigensBiotinImmunoenzyme TechniquesInmunoensayosAntigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratioInstituto de Tecnología de AlimentosFil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; ArgentinaFil: Tezon, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaTaylor & Francis2018-11-27T14:44:34Z2018-11-27T14:44:34Z2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.tandfonline.com/doi/full/10.1080/09540105.2011.629314http://hdl.handle.net/20.500.12123/39770954-01051465-3443https://doi.org/10.1080/09540105.2011.629314Food and Agricultural Immunology 23 (4) : 315-328 (December 2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:30Zoai:localhost:20.500.12123/3977instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:30.967INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
title Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
spellingShingle Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
Spotorno, Viviana Graciela
Técnicas Inmunológicas
Antígenos
Biotina
Técnicas Inmunoenzimáticas
Clenbuterol
Immunological Techniques
Antigens
Biotin
Immunoenzyme Techniques
Inmunoensayos
title_short Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
title_full Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
title_fullStr Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
title_full_unstemmed Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
title_sort Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
dc.creator.none.fl_str_mv Spotorno, Viviana Graciela
Tezón, Jorge Guillermo
author Spotorno, Viviana Graciela
author_facet Spotorno, Viviana Graciela
Tezón, Jorge Guillermo
author_role author
author2 Tezón, Jorge Guillermo
author2_role author
dc.subject.none.fl_str_mv Técnicas Inmunológicas
Antígenos
Biotina
Técnicas Inmunoenzimáticas
Clenbuterol
Immunological Techniques
Antigens
Biotin
Immunoenzyme Techniques
Inmunoensayos
topic Técnicas Inmunológicas
Antígenos
Biotina
Técnicas Inmunoenzimáticas
Clenbuterol
Immunological Techniques
Antigens
Biotin
Immunoenzyme Techniques
Inmunoensayos
dc.description.none.fl_txt_mv Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratio
Instituto de Tecnología de Alimentos
Fil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
Fil: Tezon, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratio
publishDate 2012
dc.date.none.fl_str_mv 2012-12
2018-11-27T14:44:34Z
2018-11-27T14:44:34Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.tandfonline.com/doi/full/10.1080/09540105.2011.629314
http://hdl.handle.net/20.500.12123/3977
0954-0105
1465-3443
https://doi.org/10.1080/09540105.2011.629314
url https://www.tandfonline.com/doi/full/10.1080/09540105.2011.629314
http://hdl.handle.net/20.500.12123/3977
https://doi.org/10.1080/09540105.2011.629314
identifier_str_mv 0954-0105
1465-3443
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv Food and Agricultural Immunology 23 (4) : 315-328 (December 2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.891075

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