In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic

Autores
Esteban, Micaela; Saraullo, Vanina Rosa; Madero, Agustin; Hamer, Micaela; Sanchez, Maria Cristina; Brihuega, Bibiana Felicitas; Martinez, Mara Leila
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Poster
Leptospirosis is a zoonotic infectious disease caused by the spirochete of the genus Leptospira. The gold-standard technique for diagnosis is the microscopic agglutina tion test, however, this serological test is complex and time consuming, and requires specific equipment, highly trained staff, and the live cultures of reference strains of Leptospira for use as antigens. It is for this reason that the search for antigenic can didates for immunodiagnostics takes on significant importance. LipL32 is an outer membrane lipoprotein that is exclusively found in pathogenic leptospires, highly con served and immunogenic. In this study, in silico analysis of the amino acid sequence of LipL32 was carried out with the aim of finding peptides that are good candidates for diagnostic use, bound to gold nanoparticles. First, amino acid sequences of lipL32 were searched in Genbank and conservation in pathogenic leptospires (Pomona and Hardjo) was compared between them, using the MUSCLE clustal platform. The signal peptide region was determined by DTU health tech as it is ruled out as an anti gen. Remaining amino acid sequence was evaluated in different predictors of epi topes (EMBOSS, Antibody Epitope Prediction, SVMTrip, Predicted Antigenic Peptides, ABC Pred). Once the results of the predictors were obtained, they were compared with each other. The peptides that matched in most of the predictors were selected and evaluated with PepCalc for molecular weight and water solubility.
Instituto de Patobiología
Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Madero, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Madero, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina
Fuente
The 13th Conference of the International Leptospirosis Society and The 4th Meeting of the European Leptospirosis and other Roden-Born Haemorrhagic fevers Society, Brussels, 2-4 September 2024
Materia
Peptides
Antigens
Leptospirosis
Immunodiagnosis
Péptidos
Antígenos
Inmunodiagnóstico
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/20487
Acceder
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oai_identifier_str oai:localhost:20.500.12123/20487
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnosticEsteban, MicaelaSaraullo, Vanina RosaMadero, AgustinHamer, MicaelaSanchez, Maria CristinaBrihuega, Bibiana FelicitasMartinez, Mara LeilaPeptidesAntigensLeptospirosisImmunodiagnosisPéptidosAntígenosInmunodiagnósticoPosterLeptospirosis is a zoonotic infectious disease caused by the spirochete of the genus Leptospira. The gold-standard technique for diagnosis is the microscopic agglutina tion test, however, this serological test is complex and time consuming, and requires specific equipment, highly trained staff, and the live cultures of reference strains of Leptospira for use as antigens. It is for this reason that the search for antigenic can didates for immunodiagnostics takes on significant importance. LipL32 is an outer membrane lipoprotein that is exclusively found in pathogenic leptospires, highly con served and immunogenic. In this study, in silico analysis of the amino acid sequence of LipL32 was carried out with the aim of finding peptides that are good candidates for diagnostic use, bound to gold nanoparticles. First, amino acid sequences of lipL32 were searched in Genbank and conservation in pathogenic leptospires (Pomona and Hardjo) was compared between them, using the MUSCLE clustal platform. The signal peptide region was determined by DTU health tech as it is ruled out as an anti gen. Remaining amino acid sequence was evaluated in different predictors of epi topes (EMBOSS, Antibody Epitope Prediction, SVMTrip, Predicted Antigenic Peptides, ABC Pred). Once the results of the predictors were obtained, they were compared with each other. The peptides that matched in most of the predictors were selected and evaluated with PepCalc for molecular weight and water solubility.Instituto de PatobiologíaFil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Madero, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Madero, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; ArgentinaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; ArgentinaInternational Leptospirosis Society2024-12-05T11:34:21Z2024-12-05T11:34:21Z2024-09info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/20487The 13th Conference of the International Leptospirosis Society and The 4th Meeting of the European Leptospirosis and other Roden-Born Haemorrhagic fevers Society, Brussels, 2-4 September 2024reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:03Zoai:localhost:20.500.12123/20487instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:03.605INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
title In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
spellingShingle In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
Esteban, Micaela
Peptides
Antigens
Leptospirosis
Immunodiagnosis
Péptidos
Antígenos
Inmunodiagnóstico
title_short In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
title_full In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
title_fullStr In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
title_full_unstemmed In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
title_sort In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
dc.creator.none.fl_str_mv Esteban, Micaela
Saraullo, Vanina Rosa
Madero, Agustin
Hamer, Micaela
Sanchez, Maria Cristina
Brihuega, Bibiana Felicitas
Martinez, Mara Leila
author Esteban, Micaela
author_facet Esteban, Micaela
Saraullo, Vanina Rosa
Madero, Agustin
Hamer, Micaela
Sanchez, Maria Cristina
Brihuega, Bibiana Felicitas
Martinez, Mara Leila
author_role author
author2 Saraullo, Vanina Rosa
Madero, Agustin
Hamer, Micaela
Sanchez, Maria Cristina
Brihuega, Bibiana Felicitas
Martinez, Mara Leila
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Peptides
Antigens
Leptospirosis
Immunodiagnosis
Péptidos
Antígenos
Inmunodiagnóstico
topic Peptides
Antigens
Leptospirosis
Immunodiagnosis
Péptidos
Antígenos
Inmunodiagnóstico
dc.description.none.fl_txt_mv Poster
Leptospirosis is a zoonotic infectious disease caused by the spirochete of the genus Leptospira. The gold-standard technique for diagnosis is the microscopic agglutina tion test, however, this serological test is complex and time consuming, and requires specific equipment, highly trained staff, and the live cultures of reference strains of Leptospira for use as antigens. It is for this reason that the search for antigenic can didates for immunodiagnostics takes on significant importance. LipL32 is an outer membrane lipoprotein that is exclusively found in pathogenic leptospires, highly con served and immunogenic. In this study, in silico analysis of the amino acid sequence of LipL32 was carried out with the aim of finding peptides that are good candidates for diagnostic use, bound to gold nanoparticles. First, amino acid sequences of lipL32 were searched in Genbank and conservation in pathogenic leptospires (Pomona and Hardjo) was compared between them, using the MUSCLE clustal platform. The signal peptide region was determined by DTU health tech as it is ruled out as an anti gen. Remaining amino acid sequence was evaluated in different predictors of epi topes (EMBOSS, Antibody Epitope Prediction, SVMTrip, Predicted Antigenic Peptides, ABC Pred). Once the results of the predictors were obtained, they were compared with each other. The peptides that matched in most of the predictors were selected and evaluated with PepCalc for molecular weight and water solubility.
Instituto de Patobiología
Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Madero, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Madero, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina
description Poster
publishDate 2024
dc.date.none.fl_str_mv 2024-12-05T11:34:21Z
2024-12-05T11:34:21Z
2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/20487
url http://hdl.handle.net/20.500.12123/20487
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Leptospirosis Society
publisher.none.fl_str_mv International Leptospirosis Society
dc.source.none.fl_str_mv The 13th Conference of the International Leptospirosis Society and The 4th Meeting of the European Leptospirosis and other Roden-Born Haemorrhagic fevers Society, Brussels, 2-4 September 2024
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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