Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina

Autores
Vasini Rosell, Brenda; Farace, Pablo Daniel; Amadio, Ariel; Cirone, Karina Mariela; Mendez, Laura Luján; Morsella, Claudia Graciela; Fresia, Pablo; Iraola, Gregorio; Gioffre, Andrea; Paolicchi, Fernando
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paratuberculosis is a worldwide chronic enteric disease of ruminants, caused by Mycobacterium avium subsp. paratuberculosis (MAP). While MAP has been widely investigated all around the world, little is known about the different strains that circulate in each country. This study describes the genetic diversity of MAP isolates from different bovine and deer herds from Argentina, analyzed by Multiple-Locus Variable number tandem repeat Analysis (MLVA), as well as the phylogenetic relatedness between geographically distant isolates through Whole Genome Sequencing (WGS) and core-genome analysis. A total of 90 MAP isolates were analyzed. The results showed seven different MLVA genotypes, with almost 75% of them belonging to pattern INMV 1, described in all the herds studied. WGS results suggested the presence of a common INMV 1 strain circulating throughout the country. Our results allow confirming the coexistence of different strains in time and space and the mixed infections identified in some animals. These observations suggest the absence of animal monitoring prior to introduction to the herds and the need for a control program in the country. This study represents the first to report WGS of MAP strains in Argentina.
EEA Balcarce
Fil: Vasini Rosell, Brenda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Vasini Rosell, Brenda. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Vasini Rosell, Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Cirone, Karina Mariela. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Mendez, Laura Luján. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Morsella, Claudia Graciela. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Fresia, Pablo. Institut Pasteur Montevideo. Microbial Genomics Laboratory; Uruguay
Fil: Fresia, Pablo. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay
Fil: Iraola, Gregorio. Institut Pasteur Montevideo. Microbial Genomics Laboratory; Uruguay
Fil: Iraola, Gregorio. Universidad Mayor. Center for Integrative Biology; Chile
Fil: Iraola, Gregorio. Wellcome Sanger Institute; Reino Unido
Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fuente
Veterinary Research Communications 46 (4) : 1121-1129. (December 2022)
Materia
Filogenética
Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Rumiante
Paratuberculosis
Phylogenetics
Animal Diseases
Ruminants
Multiple-Locus Variable number tandem repeat Analysis (MLVA)
Whole Genome Sequencing (WGS)
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25490
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25490
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from ArgentinaVasini Rosell, BrendaFarace, Pablo DanielAmadio, ArielCirone, Karina MarielaMendez, Laura LujánMorsella, Claudia GracielaFresia, PabloIraola, GregorioGioffre, AndreaPaolicchi, FernandoFilogenéticaMycobacterium avium subsp. paratuberculosisEnfermedades de los AnimalesRumianteParatuberculosisPhylogeneticsAnimal DiseasesRuminantsMultiple-Locus Variable number tandem repeat Analysis (MLVA)Whole Genome Sequencing (WGS)Paratuberculosis is a worldwide chronic enteric disease of ruminants, caused by Mycobacterium avium subsp. paratuberculosis (MAP). While MAP has been widely investigated all around the world, little is known about the different strains that circulate in each country. This study describes the genetic diversity of MAP isolates from different bovine and deer herds from Argentina, analyzed by Multiple-Locus Variable number tandem repeat Analysis (MLVA), as well as the phylogenetic relatedness between geographically distant isolates through Whole Genome Sequencing (WGS) and core-genome analysis. A total of 90 MAP isolates were analyzed. The results showed seven different MLVA genotypes, with almost 75% of them belonging to pattern INMV 1, described in all the herds studied. WGS results suggested the presence of a common INMV 1 strain circulating throughout the country. Our results allow confirming the coexistence of different strains in time and space and the mixed infections identified in some animals. These observations suggest the absence of animal monitoring prior to introduction to the herds and the need for a control program in the country. This study represents the first to report WGS of MAP strains in Argentina.EEA BalcarceFil: Vasini Rosell, Brenda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Vasini Rosell, Brenda. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; ArgentinaFil: Vasini Rosell, Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Cirone, Karina Mariela. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; ArgentinaFil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Mendez, Laura Luján. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; ArgentinaFil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Morsella, Claudia Graciela. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; ArgentinaFil: Fresia, Pablo. Institut Pasteur Montevideo. Microbial Genomics Laboratory; UruguayFil: Fresia, Pablo. Instituto Nacional de Investigación Agropecuaria (INIA); UruguayFil: Iraola, Gregorio. Institut Pasteur Montevideo. Microbial Genomics Laboratory; UruguayFil: Iraola, Gregorio. Universidad Mayor. Center for Integrative Biology; ChileFil: Iraola, Gregorio. Wellcome Sanger Institute; Reino UnidoFil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; ArgentinaFil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; ArgentinaSpringer2026-03-17T18:09:50Z2026-03-17T18:09:50Z2022-08-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25490https://link.springer.com/article/10.1007/s11259-022-09983-81573-7446 (en línea)0165-7380 (impreso)https://doi.org/10.1007/s11259-022-09983-8Veterinary Research Communications 46 (4) : 1121-1129. (December 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicaArgentina .......... (nation) (World, South America)7006477info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-03-26T11:25:30Zoai:localhost:20.500.12123/25490instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-03-26 11:25:31.18INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
title Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
spellingShingle Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
Vasini Rosell, Brenda
Filogenética
Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Rumiante
Paratuberculosis
Phylogenetics
Animal Diseases
Ruminants
Multiple-Locus Variable number tandem repeat Analysis (MLVA)
Whole Genome Sequencing (WGS)
title_short Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
title_full Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
title_fullStr Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
title_full_unstemmed Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
title_sort Phylogenetic and multiple‑locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina
dc.creator.none.fl_str_mv Vasini Rosell, Brenda
Farace, Pablo Daniel
Amadio, Ariel
Cirone, Karina Mariela
Mendez, Laura Luján
Morsella, Claudia Graciela
Fresia, Pablo
Iraola, Gregorio
Gioffre, Andrea
Paolicchi, Fernando
author Vasini Rosell, Brenda
author_facet Vasini Rosell, Brenda
Farace, Pablo Daniel
Amadio, Ariel
Cirone, Karina Mariela
Mendez, Laura Luján
Morsella, Claudia Graciela
Fresia, Pablo
Iraola, Gregorio
Gioffre, Andrea
Paolicchi, Fernando
author_role author
author2 Farace, Pablo Daniel
Amadio, Ariel
Cirone, Karina Mariela
Mendez, Laura Luján
Morsella, Claudia Graciela
Fresia, Pablo
Iraola, Gregorio
Gioffre, Andrea
Paolicchi, Fernando
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Filogenética
Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Rumiante
Paratuberculosis
Phylogenetics
Animal Diseases
Ruminants
Multiple-Locus Variable number tandem repeat Analysis (MLVA)
Whole Genome Sequencing (WGS)
topic Filogenética
Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Rumiante
Paratuberculosis
Phylogenetics
Animal Diseases
Ruminants
Multiple-Locus Variable number tandem repeat Analysis (MLVA)
Whole Genome Sequencing (WGS)
dc.description.none.fl_txt_mv Paratuberculosis is a worldwide chronic enteric disease of ruminants, caused by Mycobacterium avium subsp. paratuberculosis (MAP). While MAP has been widely investigated all around the world, little is known about the different strains that circulate in each country. This study describes the genetic diversity of MAP isolates from different bovine and deer herds from Argentina, analyzed by Multiple-Locus Variable number tandem repeat Analysis (MLVA), as well as the phylogenetic relatedness between geographically distant isolates through Whole Genome Sequencing (WGS) and core-genome analysis. A total of 90 MAP isolates were analyzed. The results showed seven different MLVA genotypes, with almost 75% of them belonging to pattern INMV 1, described in all the herds studied. WGS results suggested the presence of a common INMV 1 strain circulating throughout the country. Our results allow confirming the coexistence of different strains in time and space and the mixed infections identified in some animals. These observations suggest the absence of animal monitoring prior to introduction to the herds and the need for a control program in the country. This study represents the first to report WGS of MAP strains in Argentina.
EEA Balcarce
Fil: Vasini Rosell, Brenda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Vasini Rosell, Brenda. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Vasini Rosell, Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Cirone, Karina Mariela. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Mendez, Laura Luján. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Morsella, Claudia Graciela. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
Fil: Fresia, Pablo. Institut Pasteur Montevideo. Microbial Genomics Laboratory; Uruguay
Fil: Fresia, Pablo. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay
Fil: Iraola, Gregorio. Institut Pasteur Montevideo. Microbial Genomics Laboratory; Uruguay
Fil: Iraola, Gregorio. Universidad Mayor. Center for Integrative Biology; Chile
Fil: Iraola, Gregorio. Wellcome Sanger Institute; Reino Unido
Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Grupo de Sanidad Animal; Argentina
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata (UNMdP). Laboratorio de Bacteriología; Argentina
description Paratuberculosis is a worldwide chronic enteric disease of ruminants, caused by Mycobacterium avium subsp. paratuberculosis (MAP). While MAP has been widely investigated all around the world, little is known about the different strains that circulate in each country. This study describes the genetic diversity of MAP isolates from different bovine and deer herds from Argentina, analyzed by Multiple-Locus Variable number tandem repeat Analysis (MLVA), as well as the phylogenetic relatedness between geographically distant isolates through Whole Genome Sequencing (WGS) and core-genome analysis. A total of 90 MAP isolates were analyzed. The results showed seven different MLVA genotypes, with almost 75% of them belonging to pattern INMV 1, described in all the herds studied. WGS results suggested the presence of a common INMV 1 strain circulating throughout the country. Our results allow confirming the coexistence of different strains in time and space and the mixed infections identified in some animals. These observations suggest the absence of animal monitoring prior to introduction to the herds and the need for a control program in the country. This study represents the first to report WGS of MAP strains in Argentina.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-11
2026-03-17T18:09:50Z
2026-03-17T18:09:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25490
https://link.springer.com/article/10.1007/s11259-022-09983-8
1573-7446 (en línea)
0165-7380 (impreso)
https://doi.org/10.1007/s11259-022-09983-8
url http://hdl.handle.net/20.500.12123/25490
https://link.springer.com/article/10.1007/s11259-022-09983-8
https://doi.org/10.1007/s11259-022-09983-8
identifier_str_mv 1573-7446 (en línea)
0165-7380 (impreso)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina .......... (nation) (World, South America)
7006477
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Veterinary Research Communications 46 (4) : 1121-1129. (December 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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