Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑...

Autores
Moyano, Roberto Damian; Imperiale, Belén Rocío; Romero, Magali Andrea; Santangelo, María de la Paz; Alvarado Pinedo, María Fiorella; Travería, Gabriel Eduardo; Romano, Maria Isabel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates.Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit?variable number tandem repeat (MIRU‑VNTR) (?automated and ?non‑automated?) and the multi‑locus short‑sequence repeat (MLSSR) system. Results: Thirty‑two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA‑restriction enzyme analysis. The main patterns found by both MIRU‑VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU‑VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 ?G? repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the ?automated? MIRU‑VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Romero, Magali Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Travería, Gabriel Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
AUTOMATED MYCOBACTERIAL INTERSPERSED REPETITIVE UNITVARIABLE NUMBER TANDEM REPEAT TYPING
GENETIC DIVERSITY
MULTI‑LOCUS SHORT‑SEQUENCE REPEAT TYPING
MYCOBACTERIUM AVIUM SP. PARATUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156511
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/156511
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis IsolatesMoyano, Roberto DamianImperiale, Belén RocíoRomero, Magali AndreaSantangelo, María de la PazAlvarado Pinedo, María FiorellaTravería, Gabriel EduardoRomano, Maria IsabelAUTOMATED MYCOBACTERIAL INTERSPERSED REPETITIVE UNITVARIABLE NUMBER TANDEM REPEAT TYPINGGENETIC DIVERSITYMULTI‑LOCUS SHORT‑SEQUENCE REPEAT TYPINGMYCOBACTERIUM AVIUM SP. PARATUBERCULOSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates.Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit?variable number tandem repeat (MIRU‑VNTR) (?automated and ?non‑automated?) and the multi‑locus short‑sequence repeat (MLSSR) system. Results: Thirty‑two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA‑restriction enzyme analysis. The main patterns found by both MIRU‑VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU‑VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 ?G? repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the ?automated? MIRU‑VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Romero, Magali Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Travería, Gabriel Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaMedknow Publications2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156511Moyano, Roberto Damian; Imperiale, Belén Rocío; Romero, Magali Andrea; Santangelo, María de la Paz; Alvarado Pinedo, María Fiorella; et al.; Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates; Medknow Publications; International Journal of Mycobacteriology; 10; 1; 3-2021; 51-592212-554XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyanoinfo:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_229_20info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:55Zoai:ri.conicet.gov.ar:11336/156511instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:55.811CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
title Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
spellingShingle Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
Moyano, Roberto Damian
AUTOMATED MYCOBACTERIAL INTERSPERSED REPETITIVE UNITVARIABLE NUMBER TANDEM REPEAT TYPING
GENETIC DIVERSITY
MULTI‑LOCUS SHORT‑SEQUENCE REPEAT TYPING
MYCOBACTERIUM AVIUM SP. PARATUBERCULOSIS
title_short Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
title_full Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
title_fullStr Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
title_full_unstemmed Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
title_sort Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates
dc.creator.none.fl_str_mv Moyano, Roberto Damian
Imperiale, Belén Rocío
Romero, Magali Andrea
Santangelo, María de la Paz
Alvarado Pinedo, María Fiorella
Travería, Gabriel Eduardo
Romano, Maria Isabel
author Moyano, Roberto Damian
author_facet Moyano, Roberto Damian
Imperiale, Belén Rocío
Romero, Magali Andrea
Santangelo, María de la Paz
Alvarado Pinedo, María Fiorella
Travería, Gabriel Eduardo
Romano, Maria Isabel
author_role author
author2 Imperiale, Belén Rocío
Romero, Magali Andrea
Santangelo, María de la Paz
Alvarado Pinedo, María Fiorella
Travería, Gabriel Eduardo
Romano, Maria Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AUTOMATED MYCOBACTERIAL INTERSPERSED REPETITIVE UNITVARIABLE NUMBER TANDEM REPEAT TYPING
GENETIC DIVERSITY
MULTI‑LOCUS SHORT‑SEQUENCE REPEAT TYPING
MYCOBACTERIUM AVIUM SP. PARATUBERCULOSIS
topic AUTOMATED MYCOBACTERIAL INTERSPERSED REPETITIVE UNITVARIABLE NUMBER TANDEM REPEAT TYPING
GENETIC DIVERSITY
MULTI‑LOCUS SHORT‑SEQUENCE REPEAT TYPING
MYCOBACTERIUM AVIUM SP. PARATUBERCULOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates.Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit?variable number tandem repeat (MIRU‑VNTR) (?automated and ?non‑automated?) and the multi‑locus short‑sequence repeat (MLSSR) system. Results: Thirty‑two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA‑restriction enzyme analysis. The main patterns found by both MIRU‑VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU‑VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 ?G? repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the ?automated? MIRU‑VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Romero, Magali Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Travería, Gabriel Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates.Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit?variable number tandem repeat (MIRU‑VNTR) (?automated and ?non‑automated?) and the multi‑locus short‑sequence repeat (MLSSR) system. Results: Thirty‑two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA‑restriction enzyme analysis. The main patterns found by both MIRU‑VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU‑VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 ?G? repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the ?automated? MIRU‑VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156511
Moyano, Roberto Damian; Imperiale, Belén Rocío; Romero, Magali Andrea; Santangelo, María de la Paz; Alvarado Pinedo, María Fiorella; et al.; Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates; Medknow Publications; International Journal of Mycobacteriology; 10; 1; 3-2021; 51-59
2212-554X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156511
identifier_str_mv Moyano, Roberto Damian; Imperiale, Belén Rocío; Romero, Magali Andrea; Santangelo, María de la Paz; Alvarado Pinedo, María Fiorella; et al.; Genetic Diversity of Mycobacterium avium sp. Paratuberculosis by Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat and Multi‑Locus Short‑Sequence Repeat One‑Sentence Summary: Genetic Diversity of Mycobacterium avium sp. Paratuberculosis Isolates; Medknow Publications; International Journal of Mycobacteriology; 10; 1; 3-2021; 51-59
2212-554X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyano
info:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_229_20
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Medknow Publications
publisher.none.fl_str_mv Medknow Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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