First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization

Autores
Fiorentino, Maria Andrea; Gioffré, Andrea Karina; Cirone, K.; Morsella, C.; Alonso, B.; Delgado, Fernando Oscar; Paolicchi, F.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
No data concerning the presence of caprine paratuberculosis (PTBC) in Argentina are currently available. In this work, a Saanen dairy goat herd located in Buenos Aires province was sampled and tested for PTBC. Feces and blood samples were collected from 210 goats.Sera were analyzed by ELISA and fecal samples were pooled for culture. Necropsy was performed in two affected adult goats. Colonies were characterized and genotyped by IS900 PCR and IS1311 PCR-REA, respectively. Gross pathology and histopathology of necropsied goats were consistent with PTBC. Bacteriology, serology, and PCR confirmed the diagnosis. The Mycobacterium avium subsp. paratuberculosis isolates showed a restriction pattern characteristic of type C strains. This is the first report on the isolation and molecular characterization of M. avium subsp. paratuberculosis in dairy goats in Argentina.
Fil: Fiorentino, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gioffré, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cirone, K.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Morsella, C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Alonso, B.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paolicchi, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Materia
DAIRY GOATS
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
PARATUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195139
Acceder
id CONICETDig_28fa736ed902803338d20cefef31810a
oai_identifier_str oai:ri.conicet.gov.ar:11336/195139
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterizationFiorentino, Maria AndreaGioffré, Andrea KarinaCirone, K.Morsella, C.Alonso, B.Delgado, Fernando OscarPaolicchi, F.DAIRY GOATSMYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSISPARATUBERCULOSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4No data concerning the presence of caprine paratuberculosis (PTBC) in Argentina are currently available. In this work, a Saanen dairy goat herd located in Buenos Aires province was sampled and tested for PTBC. Feces and blood samples were collected from 210 goats.Sera were analyzed by ELISA and fecal samples were pooled for culture. Necropsy was performed in two affected adult goats. Colonies were characterized and genotyped by IS900 PCR and IS1311 PCR-REA, respectively. Gross pathology and histopathology of necropsied goats were consistent with PTBC. Bacteriology, serology, and PCR confirmed the diagnosis. The Mycobacterium avium subsp. paratuberculosis isolates showed a restriction pattern characteristic of type C strains. This is the first report on the isolation and molecular characterization of M. avium subsp. paratuberculosis in dairy goats in Argentina.Fil: Fiorentino, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gioffré, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cirone, K.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Morsella, C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Alonso, B.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paolicchi, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaElsevier Science2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195139Fiorentino, Maria Andrea; Gioffré, Andrea Karina; Cirone, K.; Morsella, C.; Alonso, B.; et al.; First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization; Elsevier Science; Journal of Small Ruminant Research; 108; 1-3; 7-2012; 133-1360921-4488CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0921448812002520info:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2012.06.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:51Zoai:ri.conicet.gov.ar:11336/195139instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:51.789CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
title First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
spellingShingle First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
Fiorentino, Maria Andrea
DAIRY GOATS
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
PARATUBERCULOSIS
title_short First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
title_full First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
title_fullStr First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
title_full_unstemmed First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
title_sort First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization
dc.creator.none.fl_str_mv Fiorentino, Maria Andrea
Gioffré, Andrea Karina
Cirone, K.
Morsella, C.
Alonso, B.
Delgado, Fernando Oscar
Paolicchi, F.
author Fiorentino, Maria Andrea
author_facet Fiorentino, Maria Andrea
Gioffré, Andrea Karina
Cirone, K.
Morsella, C.
Alonso, B.
Delgado, Fernando Oscar
Paolicchi, F.
author_role author
author2 Gioffré, Andrea Karina
Cirone, K.
Morsella, C.
Alonso, B.
Delgado, Fernando Oscar
Paolicchi, F.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DAIRY GOATS
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
PARATUBERCULOSIS
topic DAIRY GOATS
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
PARATUBERCULOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv No data concerning the presence of caprine paratuberculosis (PTBC) in Argentina are currently available. In this work, a Saanen dairy goat herd located in Buenos Aires province was sampled and tested for PTBC. Feces and blood samples were collected from 210 goats.Sera were analyzed by ELISA and fecal samples were pooled for culture. Necropsy was performed in two affected adult goats. Colonies were characterized and genotyped by IS900 PCR and IS1311 PCR-REA, respectively. Gross pathology and histopathology of necropsied goats were consistent with PTBC. Bacteriology, serology, and PCR confirmed the diagnosis. The Mycobacterium avium subsp. paratuberculosis isolates showed a restriction pattern characteristic of type C strains. This is the first report on the isolation and molecular characterization of M. avium subsp. paratuberculosis in dairy goats in Argentina.
Fil: Fiorentino, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gioffré, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cirone, K.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Morsella, C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Alonso, B.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paolicchi, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
description No data concerning the presence of caprine paratuberculosis (PTBC) in Argentina are currently available. In this work, a Saanen dairy goat herd located in Buenos Aires province was sampled and tested for PTBC. Feces and blood samples were collected from 210 goats.Sera were analyzed by ELISA and fecal samples were pooled for culture. Necropsy was performed in two affected adult goats. Colonies were characterized and genotyped by IS900 PCR and IS1311 PCR-REA, respectively. Gross pathology and histopathology of necropsied goats were consistent with PTBC. Bacteriology, serology, and PCR confirmed the diagnosis. The Mycobacterium avium subsp. paratuberculosis isolates showed a restriction pattern characteristic of type C strains. This is the first report on the isolation and molecular characterization of M. avium subsp. paratuberculosis in dairy goats in Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195139
Fiorentino, Maria Andrea; Gioffré, Andrea Karina; Cirone, K.; Morsella, C.; Alonso, B.; et al.; First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization; Elsevier Science; Journal of Small Ruminant Research; 108; 1-3; 7-2012; 133-136
0921-4488
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195139
identifier_str_mv Fiorentino, Maria Andrea; Gioffré, Andrea Karina; Cirone, K.; Morsella, C.; Alonso, B.; et al.; First isolation of Mycobacterium avium subsp. paratuberculosis in a dairy goat in Argentina: Pathology and molecular characterization; Elsevier Science; Journal of Small Ruminant Research; 108; 1-3; 7-2012; 133-136
0921-4488
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0921448812002520
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2012.06.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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