Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis

Autores
Souza, Giliane S.; Rodrigues, Ana Bárbara F.; Gioffré, Andrea Karina; Romano, Maria Isabel; Carvalho, Eulógio C. Q.; Ventura, Thatiana L. B.; Lasunskaia, Elena B.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Comparative genomics of Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of M. avium and M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/ModD gene of M. avium subsp. paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the M. avium complex, specifically Map and M. avium subsp. hominissuis, and which did not cross-react with M. bovis or M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic M. avium subspecies via Western blotting.
Fil: Souza, Giliane S.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Rodrigues, Ana Bárbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Gioffré, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carvalho, Eulógio C. Q.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Ventura, Thatiana L. B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Materia
ANTIGEN APA
IMMUNODETECTION
M. AVIUM SUBSP. PARATUBERCULOSIS
MONOCLONAL ANTIBODIES
PARATUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192762
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192762
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosisSouza, Giliane S.Rodrigues, Ana Bárbara F.Gioffré, Andrea KarinaRomano, Maria IsabelCarvalho, Eulógio C. Q.Ventura, Thatiana L. B.Lasunskaia, Elena B.ANTIGEN APAIMMUNODETECTIONM. AVIUM SUBSP. PARATUBERCULOSISMONOCLONAL ANTIBODIESPARATUBERCULOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Comparative genomics of Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of M. avium and M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/ModD gene of M. avium subsp. paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the M. avium complex, specifically Map and M. avium subsp. hominissuis, and which did not cross-react with M. bovis or M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic M. avium subspecies via Western blotting.Fil: Souza, Giliane S.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Rodrigues, Ana Bárbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Gioffré, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carvalho, Eulógio C. Q.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Ventura, Thatiana L. B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilElsevier Science2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192762Souza, Giliane S.; Rodrigues, Ana Bárbara F.; Gioffré, Andrea Karina; Romano, Maria Isabel; Carvalho, Eulógio C. Q.; et al.; Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis; Elsevier Science; Veterinary Immunology And Immunopathology; 143; 1-2; 6-2011; 75-820165-2427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165242711002248info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2011.06.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:13:03Zoai:ri.conicet.gov.ar:11336/192762instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:13:03.619CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
title Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
spellingShingle Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
Souza, Giliane S.
ANTIGEN APA
IMMUNODETECTION
M. AVIUM SUBSP. PARATUBERCULOSIS
MONOCLONAL ANTIBODIES
PARATUBERCULOSIS
title_short Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
title_full Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
title_fullStr Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
title_full_unstemmed Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
title_sort Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis
dc.creator.none.fl_str_mv Souza, Giliane S.
Rodrigues, Ana Bárbara F.
Gioffré, Andrea Karina
Romano, Maria Isabel
Carvalho, Eulógio C. Q.
Ventura, Thatiana L. B.
Lasunskaia, Elena B.
author Souza, Giliane S.
author_facet Souza, Giliane S.
Rodrigues, Ana Bárbara F.
Gioffré, Andrea Karina
Romano, Maria Isabel
Carvalho, Eulógio C. Q.
Ventura, Thatiana L. B.
Lasunskaia, Elena B.
author_role author
author2 Rodrigues, Ana Bárbara F.
Gioffré, Andrea Karina
Romano, Maria Isabel
Carvalho, Eulógio C. Q.
Ventura, Thatiana L. B.
Lasunskaia, Elena B.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIGEN APA
IMMUNODETECTION
M. AVIUM SUBSP. PARATUBERCULOSIS
MONOCLONAL ANTIBODIES
PARATUBERCULOSIS
topic ANTIGEN APA
IMMUNODETECTION
M. AVIUM SUBSP. PARATUBERCULOSIS
MONOCLONAL ANTIBODIES
PARATUBERCULOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Comparative genomics of Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of M. avium and M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/ModD gene of M. avium subsp. paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the M. avium complex, specifically Map and M. avium subsp. hominissuis, and which did not cross-react with M. bovis or M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic M. avium subspecies via Western blotting.
Fil: Souza, Giliane S.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Rodrigues, Ana Bárbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Gioffré, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carvalho, Eulógio C. Q.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Ventura, Thatiana L. B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
description Comparative genomics of Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of M. avium and M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/ModD gene of M. avium subsp. paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the M. avium complex, specifically Map and M. avium subsp. hominissuis, and which did not cross-react with M. bovis or M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic M. avium subspecies via Western blotting.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192762
Souza, Giliane S.; Rodrigues, Ana Bárbara F.; Gioffré, Andrea Karina; Romano, Maria Isabel; Carvalho, Eulógio C. Q.; et al.; Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis; Elsevier Science; Veterinary Immunology And Immunopathology; 143; 1-2; 6-2011; 75-82
0165-2427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192762
identifier_str_mv Souza, Giliane S.; Rodrigues, Ana Bárbara F.; Gioffré, Andrea Karina; Romano, Maria Isabel; Carvalho, Eulógio C. Q.; et al.; Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis; Elsevier Science; Veterinary Immunology And Immunopathology; 143; 1-2; 6-2011; 75-82
0165-2427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165242711002248
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2011.06.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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