Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase

Autores
Escudero, Carla Gimena; Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Bazzocchini, Vanesa Silvana; García, Sebastián; Yunes, Roberto Miguel Federico; Cabrera Kreiker, Ricardo Jorge
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we investigated how the neurosteroid allopregnanolone can modulate learning and memory processes. For this purpose, we used ovariectomized (OVX) rats subcutaneously injected with oestradiol benzoate (E) alone or E and progesterone (P). Then, rats were injected in dorsal hippocampus with allopregnanolone or vehicle. Animals were tested in inhibitory avoidance task (IA task). After behavioural test hippocampal mRNA expression and enzymatic activity of 3-HOR, the enzyme responsible of allopregnanolone synthesis, were analysed. In IA task OVX-EP rats spent less time on platform, compared to those OVX or OVX-E. Regression analyses revealed that there was a significant negative relationship between E-P infusion and performance in this task. Pre-training allopregnanolone administration to OVX-EP rats increased the time spent on the platform. Interestingly, when enzymatic activity of 3-HOR was tested, OVX-EP rats showed a significant decrease in the enzymatic activity, compared with OVX and OVX-E rats. In addition, OVX-EP group showed a significant increase in the enzymatic activity after intrahippocampal infusion of allopregnanolone. On the other hand, when mRNA expression of 3-HOR was analysed no differences were observed when the hippocampal allopregnanolone injection was done. These results suggest that E and P have amnesic effects on female rats, being reversed by allopregnanolone through its modulation on hippocampal 3-HOR activity.
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: Bazzocchini, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: García, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Yunes, Roberto Miguel Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fuente
Referido por https://doi.org/10.1016/j.brainresbull.2012.07.004
Materia
3α-Hor
3α-Hsd
Allopregnanolone
Memory
Oestrogen
Progesterone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79178
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79178
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductaseEscudero, Carla GimenaCasas, Sebastián MarceloGiuliani, Fernando AlfredoBazzocchini, Vanesa SilvanaGarcía, SebastiánYunes, Roberto Miguel FedericoCabrera Kreiker, Ricardo Jorge3α-Hor3α-HsdAllopregnanoloneMemoryOestrogenProgesteronehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In this work we investigated how the neurosteroid allopregnanolone can modulate learning and memory processes. For this purpose, we used ovariectomized (OVX) rats subcutaneously injected with oestradiol benzoate (E) alone or E and progesterone (P). Then, rats were injected in dorsal hippocampus with allopregnanolone or vehicle. Animals were tested in inhibitory avoidance task (IA task). After behavioural test hippocampal mRNA expression and enzymatic activity of 3-HOR, the enzyme responsible of allopregnanolone synthesis, were analysed. In IA task OVX-EP rats spent less time on platform, compared to those OVX or OVX-E. Regression analyses revealed that there was a significant negative relationship between E-P infusion and performance in this task. Pre-training allopregnanolone administration to OVX-EP rats increased the time spent on the platform. Interestingly, when enzymatic activity of 3-HOR was tested, OVX-EP rats showed a significant decrease in the enzymatic activity, compared with OVX and OVX-E rats. In addition, OVX-EP group showed a significant increase in the enzymatic activity after intrahippocampal infusion of allopregnanolone. On the other hand, when mRNA expression of 3-HOR was analysed no differences were observed when the hippocampal allopregnanolone injection was done. These results suggest that E and P have amnesic effects on female rats, being reversed by allopregnanolone through its modulation on hippocampal 3-HOR activity.Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; ArgentinaFil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; ArgentinaFil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; ArgentinaFil: Bazzocchini, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; ArgentinaFil: García, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Yunes, Roberto Miguel Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; ArgentinaPergamon-Elsevier Science Ltd2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79178Escudero, Carla Gimena; Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Bazzocchini, Vanesa Silvana; García, Sebastián; et al.; Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 87; 2-3; 2-2012; 280-2850361-9230CONICET DigitalCONICETReferido por https://doi.org/10.1016/j.brainresbull.2012.07.004reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicasenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainresbull.2011.11.019info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S036192301100356X?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/2025-10-15T15:14:23Zoai:ri.conicet.gov.ar:11336/79178instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:23.595CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
title Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
spellingShingle Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
Escudero, Carla Gimena
3α-Hor
3α-Hsd
Allopregnanolone
Memory
Oestrogen
Progesterone
title_short Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
title_full Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
title_fullStr Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
title_full_unstemmed Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
title_sort Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase
dc.creator.none.fl_str_mv Escudero, Carla Gimena
Casas, Sebastián Marcelo
Giuliani, Fernando Alfredo
Bazzocchini, Vanesa Silvana
García, Sebastián
Yunes, Roberto Miguel Federico
Cabrera Kreiker, Ricardo Jorge
author Escudero, Carla Gimena
author_facet Escudero, Carla Gimena
Casas, Sebastián Marcelo
Giuliani, Fernando Alfredo
Bazzocchini, Vanesa Silvana
García, Sebastián
Yunes, Roberto Miguel Federico
Cabrera Kreiker, Ricardo Jorge
author_role author
author2 Casas, Sebastián Marcelo
Giuliani, Fernando Alfredo
Bazzocchini, Vanesa Silvana
García, Sebastián
Yunes, Roberto Miguel Federico
Cabrera Kreiker, Ricardo Jorge
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv 3α-Hor
3α-Hsd
Allopregnanolone
Memory
Oestrogen
Progesterone
topic 3α-Hor
3α-Hsd
Allopregnanolone
Memory
Oestrogen
Progesterone
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this work we investigated how the neurosteroid allopregnanolone can modulate learning and memory processes. For this purpose, we used ovariectomized (OVX) rats subcutaneously injected with oestradiol benzoate (E) alone or E and progesterone (P). Then, rats were injected in dorsal hippocampus with allopregnanolone or vehicle. Animals were tested in inhibitory avoidance task (IA task). After behavioural test hippocampal mRNA expression and enzymatic activity of 3-HOR, the enzyme responsible of allopregnanolone synthesis, were analysed. In IA task OVX-EP rats spent less time on platform, compared to those OVX or OVX-E. Regression analyses revealed that there was a significant negative relationship between E-P infusion and performance in this task. Pre-training allopregnanolone administration to OVX-EP rats increased the time spent on the platform. Interestingly, when enzymatic activity of 3-HOR was tested, OVX-EP rats showed a significant decrease in the enzymatic activity, compared with OVX and OVX-E rats. In addition, OVX-EP group showed a significant increase in the enzymatic activity after intrahippocampal infusion of allopregnanolone. On the other hand, when mRNA expression of 3-HOR was analysed no differences were observed when the hippocampal allopregnanolone injection was done. These results suggest that E and P have amnesic effects on female rats, being reversed by allopregnanolone through its modulation on hippocampal 3-HOR activity.
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: Bazzocchini, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
Fil: García, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Yunes, Roberto Miguel Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza; Argentina
description In this work we investigated how the neurosteroid allopregnanolone can modulate learning and memory processes. For this purpose, we used ovariectomized (OVX) rats subcutaneously injected with oestradiol benzoate (E) alone or E and progesterone (P). Then, rats were injected in dorsal hippocampus with allopregnanolone or vehicle. Animals were tested in inhibitory avoidance task (IA task). After behavioural test hippocampal mRNA expression and enzymatic activity of 3-HOR, the enzyme responsible of allopregnanolone synthesis, were analysed. In IA task OVX-EP rats spent less time on platform, compared to those OVX or OVX-E. Regression analyses revealed that there was a significant negative relationship between E-P infusion and performance in this task. Pre-training allopregnanolone administration to OVX-EP rats increased the time spent on the platform. Interestingly, when enzymatic activity of 3-HOR was tested, OVX-EP rats showed a significant decrease in the enzymatic activity, compared with OVX and OVX-E rats. In addition, OVX-EP group showed a significant increase in the enzymatic activity after intrahippocampal infusion of allopregnanolone. On the other hand, when mRNA expression of 3-HOR was analysed no differences were observed when the hippocampal allopregnanolone injection was done. These results suggest that E and P have amnesic effects on female rats, being reversed by allopregnanolone through its modulation on hippocampal 3-HOR activity.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79178
Escudero, Carla Gimena; Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Bazzocchini, Vanesa Silvana; García, Sebastián; et al.; Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 87; 2-3; 2-2012; 280-285
0361-9230
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79178
identifier_str_mv Escudero, Carla Gimena; Casas, Sebastián Marcelo; Giuliani, Fernando Alfredo; Bazzocchini, Vanesa Silvana; García, Sebastián; et al.; Allopregnanolone prevents memory impairment: Effect on mRNA expression and enzymatic activity of hippocampal 3-α hydroxysteroid oxide-reductase; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 87; 2-3; 2-2012; 280-285
0361-9230
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainresbull.2011.11.019
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S036192301100356X?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv Referido por https://doi.org/10.1016/j.brainresbull.2012.07.004
reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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