Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.

Autores
Giuliani, Fernando Alfredo; Escudero, Carla Gimena; Casas, Sebastián Marcelo; Bazzocchini, Vanesa Silvana; Yunes, Roberto Miguel Federico; Laconi, Myriam Raquel; Cabrera Kreiker, Ricardo Jorge
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The hypothalamic release of glutamate and GABA regulates neurosecretory functions that may control the onset of puberty. This release may be influenced by neurosteroids such as allopregnanolone. Using superfusion experiments we examined the role of allopregnanolone on the K+-evoked and basal [3H]-glutamate and [3H]-GABA release from mediobasal hypothalamus and anterior preoptic area in prepubertal, vaginal opening and pubertal (P) rats and evaluated its modulatory effect on GABAA and NMDA (N-methyl-d-aspartic acid) receptors. Also, we examined the hypothalamic activity and mRNA expression of 3α-hydroxysteroid oxidoreductase (3α-HSOR) ? enzyme that synthesizes allopregnanolone ? using a spectrophotometric method and RT-PCR, respectively. Allopregnanolone increased both the K+-evoked [3H]-glutamate and [3H]-GABA release in P rats, being the former effect mediated by the modulation of NMDA receptors ? as was reverted by Mg2+ and by the NMDA receptor antagonist AP-7 and the latter by the modulation of NMDA and GABAA receptors ? as was reverted by Mg2+ and the GABAA receptor antagonist bicuculline. The neurosteroid also increased the basal release of [3H]-glutamate in VO rats in an effect that was dependent on the modulation of NMDA receptors as was reverted by Mg2+. On the other hand we show that allopregnanolone reduced the basal release of [3H]-GABA in P rats although we cannot elucidate the precise mechanism by which the neurosteroid exerted this latter effect. The enzymatic activity and the mRNA expression of 3α-HSOR were both increased in P rats regarding the other two studied stages of sexual development. These results suggest an important physiological function of allopregnanolone in the hypothalamus of the P rat where it might be involved in the ?fine tuning? of neurosecretory functions related to the biology of reproduction of the female rats.
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bazzocchini, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Área de Farmacología; Argentina
Fil: Laconi, Myriam Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
PUBERTY
ALLOPREGNANOLONE
GLUTAMATE RELEASE
GABA RELEASE
3ALPHA-HYDROXYSTEROID OXYDOREDUCTASE
HYPOTHALAMUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2314
Acceder
id CONICETDig_df6d5766eb2e437949fb5ede40cb857a
oai_identifier_str oai:ri.conicet.gov.ar:11336/2314
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.Giuliani, Fernando AlfredoEscudero, Carla GimenaCasas, Sebastián MarceloBazzocchini, Vanesa SilvanaYunes, Roberto Miguel FedericoLaconi, Myriam RaquelCabrera Kreiker, Ricardo JorgePUBERTYALLOPREGNANOLONEGLUTAMATE RELEASEGABA RELEASE3ALPHA-HYDROXYSTEROID OXYDOREDUCTASEHYPOTHALAMUShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The hypothalamic release of glutamate and GABA regulates neurosecretory functions that may control the onset of puberty. This release may be influenced by neurosteroids such as allopregnanolone. Using superfusion experiments we examined the role of allopregnanolone on the K+-evoked and basal [3H]-glutamate and [3H]-GABA release from mediobasal hypothalamus and anterior preoptic area in prepubertal, vaginal opening and pubertal (P) rats and evaluated its modulatory effect on GABAA and NMDA (N-methyl-d-aspartic acid) receptors. Also, we examined the hypothalamic activity and mRNA expression of 3α-hydroxysteroid oxidoreductase (3α-HSOR) ? enzyme that synthesizes allopregnanolone ? using a spectrophotometric method and RT-PCR, respectively. Allopregnanolone increased both the K+-evoked [3H]-glutamate and [3H]-GABA release in P rats, being the former effect mediated by the modulation of NMDA receptors ? as was reverted by Mg2+ and by the NMDA receptor antagonist AP-7 and the latter by the modulation of NMDA and GABAA receptors ? as was reverted by Mg2+ and the GABAA receptor antagonist bicuculline. The neurosteroid also increased the basal release of [3H]-glutamate in VO rats in an effect that was dependent on the modulation of NMDA receptors as was reverted by Mg2+. On the other hand we show that allopregnanolone reduced the basal release of [3H]-GABA in P rats although we cannot elucidate the precise mechanism by which the neurosteroid exerted this latter effect. The enzymatic activity and the mRNA expression of 3α-HSOR were both increased in P rats regarding the other two studied stages of sexual development. These results suggest an important physiological function of allopregnanolone in the hypothalamus of the P rat where it might be involved in the ?fine tuning? of neurosecretory functions related to the biology of reproduction of the female rats.Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Bazzocchini, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Área de Farmacología; ArgentinaFil: Laconi, Myriam Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaPergamon-Elsevier Science Ltd2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2314Giuliani, Fernando Alfredo; Escudero, Carla Gimena; Casas, Sebastián Marcelo; Bazzocchini, Vanesa Silvana; Yunes, Roberto Miguel Federico; et al.; Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.; Pergamon-Elsevier Science Ltd; Neuroscience; 243; 4-2013; 64-750306-4522enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452213003084info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2013.03.053info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:54Zoai:ri.conicet.gov.ar:11336/2314instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:54.967CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
title Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
spellingShingle Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
Giuliani, Fernando Alfredo
PUBERTY
ALLOPREGNANOLONE
GLUTAMATE RELEASE
GABA RELEASE
3ALPHA-HYDROXYSTEROID OXYDOREDUCTASE
HYPOTHALAMUS
title_short Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
title_full Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
title_fullStr Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
title_full_unstemmed Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
title_sort Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.
dc.creator.none.fl_str_mv Giuliani, Fernando Alfredo
Escudero, Carla Gimena
Casas, Sebastián Marcelo
Bazzocchini, Vanesa Silvana
Yunes, Roberto Miguel Federico
Laconi, Myriam Raquel
Cabrera Kreiker, Ricardo Jorge
author Giuliani, Fernando Alfredo
author_facet Giuliani, Fernando Alfredo
Escudero, Carla Gimena
Casas, Sebastián Marcelo
Bazzocchini, Vanesa Silvana
Yunes, Roberto Miguel Federico
Laconi, Myriam Raquel
Cabrera Kreiker, Ricardo Jorge
author_role author
author2 Escudero, Carla Gimena
Casas, Sebastián Marcelo
Bazzocchini, Vanesa Silvana
Yunes, Roberto Miguel Federico
Laconi, Myriam Raquel
Cabrera Kreiker, Ricardo Jorge
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PUBERTY
ALLOPREGNANOLONE
GLUTAMATE RELEASE
GABA RELEASE
3ALPHA-HYDROXYSTEROID OXYDOREDUCTASE
HYPOTHALAMUS
topic PUBERTY
ALLOPREGNANOLONE
GLUTAMATE RELEASE
GABA RELEASE
3ALPHA-HYDROXYSTEROID OXYDOREDUCTASE
HYPOTHALAMUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The hypothalamic release of glutamate and GABA regulates neurosecretory functions that may control the onset of puberty. This release may be influenced by neurosteroids such as allopregnanolone. Using superfusion experiments we examined the role of allopregnanolone on the K+-evoked and basal [3H]-glutamate and [3H]-GABA release from mediobasal hypothalamus and anterior preoptic area in prepubertal, vaginal opening and pubertal (P) rats and evaluated its modulatory effect on GABAA and NMDA (N-methyl-d-aspartic acid) receptors. Also, we examined the hypothalamic activity and mRNA expression of 3α-hydroxysteroid oxidoreductase (3α-HSOR) ? enzyme that synthesizes allopregnanolone ? using a spectrophotometric method and RT-PCR, respectively. Allopregnanolone increased both the K+-evoked [3H]-glutamate and [3H]-GABA release in P rats, being the former effect mediated by the modulation of NMDA receptors ? as was reverted by Mg2+ and by the NMDA receptor antagonist AP-7 and the latter by the modulation of NMDA and GABAA receptors ? as was reverted by Mg2+ and the GABAA receptor antagonist bicuculline. The neurosteroid also increased the basal release of [3H]-glutamate in VO rats in an effect that was dependent on the modulation of NMDA receptors as was reverted by Mg2+. On the other hand we show that allopregnanolone reduced the basal release of [3H]-GABA in P rats although we cannot elucidate the precise mechanism by which the neurosteroid exerted this latter effect. The enzymatic activity and the mRNA expression of 3α-HSOR were both increased in P rats regarding the other two studied stages of sexual development. These results suggest an important physiological function of allopregnanolone in the hypothalamus of the P rat where it might be involved in the ?fine tuning? of neurosecretory functions related to the biology of reproduction of the female rats.
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bazzocchini, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Área de Farmacología; Argentina
Fil: Laconi, Myriam Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description The hypothalamic release of glutamate and GABA regulates neurosecretory functions that may control the onset of puberty. This release may be influenced by neurosteroids such as allopregnanolone. Using superfusion experiments we examined the role of allopregnanolone on the K+-evoked and basal [3H]-glutamate and [3H]-GABA release from mediobasal hypothalamus and anterior preoptic area in prepubertal, vaginal opening and pubertal (P) rats and evaluated its modulatory effect on GABAA and NMDA (N-methyl-d-aspartic acid) receptors. Also, we examined the hypothalamic activity and mRNA expression of 3α-hydroxysteroid oxidoreductase (3α-HSOR) ? enzyme that synthesizes allopregnanolone ? using a spectrophotometric method and RT-PCR, respectively. Allopregnanolone increased both the K+-evoked [3H]-glutamate and [3H]-GABA release in P rats, being the former effect mediated by the modulation of NMDA receptors ? as was reverted by Mg2+ and by the NMDA receptor antagonist AP-7 and the latter by the modulation of NMDA and GABAA receptors ? as was reverted by Mg2+ and the GABAA receptor antagonist bicuculline. The neurosteroid also increased the basal release of [3H]-glutamate in VO rats in an effect that was dependent on the modulation of NMDA receptors as was reverted by Mg2+. On the other hand we show that allopregnanolone reduced the basal release of [3H]-GABA in P rats although we cannot elucidate the precise mechanism by which the neurosteroid exerted this latter effect. The enzymatic activity and the mRNA expression of 3α-HSOR were both increased in P rats regarding the other two studied stages of sexual development. These results suggest an important physiological function of allopregnanolone in the hypothalamus of the P rat where it might be involved in the ?fine tuning? of neurosecretory functions related to the biology of reproduction of the female rats.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2314
Giuliani, Fernando Alfredo; Escudero, Carla Gimena; Casas, Sebastián Marcelo; Bazzocchini, Vanesa Silvana; Yunes, Roberto Miguel Federico; et al.; Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.; Pergamon-Elsevier Science Ltd; Neuroscience; 243; 4-2013; 64-75
0306-4522
url http://hdl.handle.net/11336/2314
identifier_str_mv Giuliani, Fernando Alfredo; Escudero, Carla Gimena; Casas, Sebastián Marcelo; Bazzocchini, Vanesa Silvana; Yunes, Roberto Miguel Federico; et al.; Allopregnanolone and puberty: modulatory effect on glutamate and GABA release and expression of 3-hydroxysteroid oxidoreductase in the hypothalamus of female rats.; Pergamon-Elsevier Science Ltd; Neuroscience; 243; 4-2013; 64-75
0306-4522
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452213003084
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2013.03.053
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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