Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile

Autores
Pelegrina, Laura Tatiana; Escudero, Carla Gimena; Giuliani, Fernando Alfredo; García Menéndez, Sebastián Marcelo Manuel; Cabrera Kreiker, Ricardo Jorge; Laconi, Myriam Raquel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously observed that intracerebroventricular allopregnanolone (ALLO) injection produced an anxiolytic effect and inhibited sexual receptivity when the test was performed in a separate manner. Also, ALLO reverts learning deficit in female rats in the hippocampi. To study the behavioral effects of an acute treatment with ALLO in the right lateral ventricle we used two approaches: a- A battery test to analyze the anxiety and mating behavior. And b- The avoidance test and novel object recognition test to evaluate its effect on memory and learning. Ovariectomized rats were injected with estrogen and progesterone. After it ALLO or vehicle were administered into the right lateral ventricle. To reach the objective (a) rats were put in a sequential battery test in the next order: 1-Open field. 2- Plus maze task. 3- Mating behavior. For the aim (b) it was performed a Novel Object Recognition Test and Step-down Inhibitory Avoidance Task. ALLO did not affect locomotors-exploratory behavior. Animals treated with ALLO, spent more time and had more entries into the open arm in a plus maze task and lordosis quotient was lower than in the control group. ALLO increased the latency in step down test and had no effects on discrimination index test in NORT. Here we demonstrated that one pharmacological dose of ALLO in ovariectomized primed rats is enough to generate all changes observed in the battery test. Moreover, the acute treatment with ALLO in lateral ventricle enhanced the memory acquisition in an avoidance task.
Fil: Pelegrina, Laura Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: García Menéndez, Sebastián Marcelo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Laconi, Myriam Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
allopregnanolone
cognition
memory
behaviour
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41886
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41886
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profilePelegrina, Laura TatianaEscudero, Carla GimenaGiuliani, Fernando AlfredoGarcía Menéndez, Sebastián Marcelo ManuelCabrera Kreiker, Ricardo JorgeLaconi, Myriam Raquelallopregnanolonecognitionmemorybehaviourhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have previously observed that intracerebroventricular allopregnanolone (ALLO) injection produced an anxiolytic effect and inhibited sexual receptivity when the test was performed in a separate manner. Also, ALLO reverts learning deficit in female rats in the hippocampi. To study the behavioral effects of an acute treatment with ALLO in the right lateral ventricle we used two approaches: a- A battery test to analyze the anxiety and mating behavior. And b- The avoidance test and novel object recognition test to evaluate its effect on memory and learning. Ovariectomized rats were injected with estrogen and progesterone. After it ALLO or vehicle were administered into the right lateral ventricle. To reach the objective (a) rats were put in a sequential battery test in the next order: 1-Open field. 2- Plus maze task. 3- Mating behavior. For the aim (b) it was performed a Novel Object Recognition Test and Step-down Inhibitory Avoidance Task. ALLO did not affect locomotors-exploratory behavior. Animals treated with ALLO, spent more time and had more entries into the open arm in a plus maze task and lordosis quotient was lower than in the control group. ALLO increased the latency in step down test and had no effects on discrimination index test in NORT. Here we demonstrated that one pharmacological dose of ALLO in ovariectomized primed rats is enough to generate all changes observed in the battery test. Moreover, the acute treatment with ALLO in lateral ventricle enhanced the memory acquisition in an avoidance task.Fil: Pelegrina, Laura Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: García Menéndez, Sebastián Marcelo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Laconi, Myriam Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaInstituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis2015-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41886Pelegrina, Laura Tatiana; Escudero, Carla Gimena; Giuliani, Fernando Alfredo; García Menéndez, Sebastián Marcelo Manuel; Cabrera Kreiker, Ricardo Jorge; et al.; Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile; Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis; Brazilian Journal of Biological Sciences; 2; 3; 30-6-2015; 39-502358-2731CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://revista.rebibio.net/v2n3/v02n03a05a.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:32Zoai:ri.conicet.gov.ar:11336/41886instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:32.599CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
title Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
spellingShingle Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
Pelegrina, Laura Tatiana
allopregnanolone
cognition
memory
behaviour
title_short Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
title_full Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
title_fullStr Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
title_full_unstemmed Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
title_sort Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile
dc.creator.none.fl_str_mv Pelegrina, Laura Tatiana
Escudero, Carla Gimena
Giuliani, Fernando Alfredo
García Menéndez, Sebastián Marcelo Manuel
Cabrera Kreiker, Ricardo Jorge
Laconi, Myriam Raquel
author Pelegrina, Laura Tatiana
author_facet Pelegrina, Laura Tatiana
Escudero, Carla Gimena
Giuliani, Fernando Alfredo
García Menéndez, Sebastián Marcelo Manuel
Cabrera Kreiker, Ricardo Jorge
Laconi, Myriam Raquel
author_role author
author2 Escudero, Carla Gimena
Giuliani, Fernando Alfredo
García Menéndez, Sebastián Marcelo Manuel
Cabrera Kreiker, Ricardo Jorge
Laconi, Myriam Raquel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv allopregnanolone
cognition
memory
behaviour
topic allopregnanolone
cognition
memory
behaviour
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have previously observed that intracerebroventricular allopregnanolone (ALLO) injection produced an anxiolytic effect and inhibited sexual receptivity when the test was performed in a separate manner. Also, ALLO reverts learning deficit in female rats in the hippocampi. To study the behavioral effects of an acute treatment with ALLO in the right lateral ventricle we used two approaches: a- A battery test to analyze the anxiety and mating behavior. And b- The avoidance test and novel object recognition test to evaluate its effect on memory and learning. Ovariectomized rats were injected with estrogen and progesterone. After it ALLO or vehicle were administered into the right lateral ventricle. To reach the objective (a) rats were put in a sequential battery test in the next order: 1-Open field. 2- Plus maze task. 3- Mating behavior. For the aim (b) it was performed a Novel Object Recognition Test and Step-down Inhibitory Avoidance Task. ALLO did not affect locomotors-exploratory behavior. Animals treated with ALLO, spent more time and had more entries into the open arm in a plus maze task and lordosis quotient was lower than in the control group. ALLO increased the latency in step down test and had no effects on discrimination index test in NORT. Here we demonstrated that one pharmacological dose of ALLO in ovariectomized primed rats is enough to generate all changes observed in the battery test. Moreover, the acute treatment with ALLO in lateral ventricle enhanced the memory acquisition in an avoidance task.
Fil: Pelegrina, Laura Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: García Menéndez, Sebastián Marcelo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Laconi, Myriam Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description We have previously observed that intracerebroventricular allopregnanolone (ALLO) injection produced an anxiolytic effect and inhibited sexual receptivity when the test was performed in a separate manner. Also, ALLO reverts learning deficit in female rats in the hippocampi. To study the behavioral effects of an acute treatment with ALLO in the right lateral ventricle we used two approaches: a- A battery test to analyze the anxiety and mating behavior. And b- The avoidance test and novel object recognition test to evaluate its effect on memory and learning. Ovariectomized rats were injected with estrogen and progesterone. After it ALLO or vehicle were administered into the right lateral ventricle. To reach the objective (a) rats were put in a sequential battery test in the next order: 1-Open field. 2- Plus maze task. 3- Mating behavior. For the aim (b) it was performed a Novel Object Recognition Test and Step-down Inhibitory Avoidance Task. ALLO did not affect locomotors-exploratory behavior. Animals treated with ALLO, spent more time and had more entries into the open arm in a plus maze task and lordosis quotient was lower than in the control group. ALLO increased the latency in step down test and had no effects on discrimination index test in NORT. Here we demonstrated that one pharmacological dose of ALLO in ovariectomized primed rats is enough to generate all changes observed in the battery test. Moreover, the acute treatment with ALLO in lateral ventricle enhanced the memory acquisition in an avoidance task.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41886
Pelegrina, Laura Tatiana; Escudero, Carla Gimena; Giuliani, Fernando Alfredo; García Menéndez, Sebastián Marcelo Manuel; Cabrera Kreiker, Ricardo Jorge; et al.; Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile; Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis; Brazilian Journal of Biological Sciences; 2; 3; 30-6-2015; 39-50
2358-2731
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41886
identifier_str_mv Pelegrina, Laura Tatiana; Escudero, Carla Gimena; Giuliani, Fernando Alfredo; García Menéndez, Sebastián Marcelo Manuel; Cabrera Kreiker, Ricardo Jorge; et al.; Pharmacological effect of one icv dose of Allopregnanolone in female rat: behavioural profile; Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis; Brazilian Journal of Biological Sciences; 2; 3; 30-6-2015; 39-50
2358-2731
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://revista.rebibio.net/v2n3/v02n03a05a.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis
publisher.none.fl_str_mv Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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