Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells

Autores
Islam, Md Aminul; Albarracín, Leonardo Miguel; Melnikov, Vyacheslav; Andrade, Bruno G. N.; Cuadrat, Rafael R. C.; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In a previous work, we demonstrated that nasally administered Dolosigranulum pigrum 040417 beneficially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 3 (TLR3) and improved protection against Respiratory Syncytial Virus (RSV) in mice. In this work, we aimed to evaluate the immunomodulatory effects of D. pigrum 040417 in human respiratory epithelial cells and the potential ability of this immunobiotic bacterium to in-crease the protection against Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The respiratory commensal bacterium D. pigrum 040417 differentially modulated the production of IFN-β, IL-6, CXCL8, CCL5 and CXCL10 in the culture supernatants of Calu-3 cells stimulated with poly(I:C) or challenged with SARS-CoV-2. The differential cytokine profile induced by the 040417 strain was associated with a significant reduction in viral replication and cellular damage after coronavirus infection. Of note, D. pigrum 030918 was not able to modify the resistance of Calu-3 cells to SARS-CoV-2 infection, indicating a strain-specific immunomodulatory effect for respiratory commensal bacteria. The findings of this work improve our understanding of the immunological mechanisms involved in the modulation of respiratory immunity induced by respiratory commensal bacteria, by demonstrating their specific effect on respiratory epithelial cells. In addition, the results suggest that particular strains such as D. pigrum 040417 could be used as a promising alternative for combating SARS-CoV-2 and reducing the severity of COVID-19.
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; Rusia
Fil: Andrade, Bruno G. N.. Munster Technological University; Irlanda
Fil: Cuadrat, Rafael R. C.. Berlín Institute for Medical Systems Biology; Alemania
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
CORONAVIRUS
COVID-19
DOLOSIGRANULUM PIGRUM
NEXT GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153800
Acceder
id CONICETDig_bebecba6a0eb2ae54f3934b3a134dc12
oai_identifier_str oai:ri.conicet.gov.ar:11336/153800
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cellsIslam, Md AminulAlbarracín, Leonardo MiguelMelnikov, VyacheslavAndrade, Bruno G. N.Cuadrat, Rafael R. C.Kitazawa, HarukiVillena, Julio CesarCORONAVIRUSCOVID-19DOLOSIGRANULUM PIGRUMNEXT GENERATION PROBIOTICSRESPIRATORY COMMENSAL BACTERIARESPIRATORY EPITHELIAL CELLSSARS-COV-2https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In a previous work, we demonstrated that nasally administered Dolosigranulum pigrum 040417 beneficially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 3 (TLR3) and improved protection against Respiratory Syncytial Virus (RSV) in mice. In this work, we aimed to evaluate the immunomodulatory effects of D. pigrum 040417 in human respiratory epithelial cells and the potential ability of this immunobiotic bacterium to in-crease the protection against Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The respiratory commensal bacterium D. pigrum 040417 differentially modulated the production of IFN-β, IL-6, CXCL8, CCL5 and CXCL10 in the culture supernatants of Calu-3 cells stimulated with poly(I:C) or challenged with SARS-CoV-2. The differential cytokine profile induced by the 040417 strain was associated with a significant reduction in viral replication and cellular damage after coronavirus infection. Of note, D. pigrum 030918 was not able to modify the resistance of Calu-3 cells to SARS-CoV-2 infection, indicating a strain-specific immunomodulatory effect for respiratory commensal bacteria. The findings of this work improve our understanding of the immunological mechanisms involved in the modulation of respiratory immunity induced by respiratory commensal bacteria, by demonstrating their specific effect on respiratory epithelial cells. In addition, the results suggest that particular strains such as D. pigrum 040417 could be used as a promising alternative for combating SARS-CoV-2 and reducing the severity of COVID-19.Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; BangladeshFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; RusiaFil: Andrade, Bruno G. N.. Munster Technological University; IrlandaFil: Cuadrat, Rafael R. C.. Berlín Institute for Medical Systems Biology; AlemaniaFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMDPI AG2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153800Islam, Md Aminul; Albarracín, Leonardo Miguel; Melnikov, Vyacheslav; Andrade, Bruno G. N.; Cuadrat, Rafael R. C.; et al.; Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells; MDPI AG; Pathogens; 10; 6; 5-2021; 1-112076-08172076-0817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10060634info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/6/634info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:05Zoai:ri.conicet.gov.ar:11336/153800instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:56:05.902CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
title Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
spellingShingle Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
Islam, Md Aminul
CORONAVIRUS
COVID-19
DOLOSIGRANULUM PIGRUM
NEXT GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
title_short Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
title_full Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
title_fullStr Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
title_full_unstemmed Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
title_sort Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
dc.creator.none.fl_str_mv Islam, Md Aminul
Albarracín, Leonardo Miguel
Melnikov, Vyacheslav
Andrade, Bruno G. N.
Cuadrat, Rafael R. C.
Kitazawa, Haruki
Villena, Julio Cesar
author Islam, Md Aminul
author_facet Islam, Md Aminul
Albarracín, Leonardo Miguel
Melnikov, Vyacheslav
Andrade, Bruno G. N.
Cuadrat, Rafael R. C.
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Albarracín, Leonardo Miguel
Melnikov, Vyacheslav
Andrade, Bruno G. N.
Cuadrat, Rafael R. C.
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CORONAVIRUS
COVID-19
DOLOSIGRANULUM PIGRUM
NEXT GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
topic CORONAVIRUS
COVID-19
DOLOSIGRANULUM PIGRUM
NEXT GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In a previous work, we demonstrated that nasally administered Dolosigranulum pigrum 040417 beneficially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 3 (TLR3) and improved protection against Respiratory Syncytial Virus (RSV) in mice. In this work, we aimed to evaluate the immunomodulatory effects of D. pigrum 040417 in human respiratory epithelial cells and the potential ability of this immunobiotic bacterium to in-crease the protection against Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The respiratory commensal bacterium D. pigrum 040417 differentially modulated the production of IFN-β, IL-6, CXCL8, CCL5 and CXCL10 in the culture supernatants of Calu-3 cells stimulated with poly(I:C) or challenged with SARS-CoV-2. The differential cytokine profile induced by the 040417 strain was associated with a significant reduction in viral replication and cellular damage after coronavirus infection. Of note, D. pigrum 030918 was not able to modify the resistance of Calu-3 cells to SARS-CoV-2 infection, indicating a strain-specific immunomodulatory effect for respiratory commensal bacteria. The findings of this work improve our understanding of the immunological mechanisms involved in the modulation of respiratory immunity induced by respiratory commensal bacteria, by demonstrating their specific effect on respiratory epithelial cells. In addition, the results suggest that particular strains such as D. pigrum 040417 could be used as a promising alternative for combating SARS-CoV-2 and reducing the severity of COVID-19.
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; Rusia
Fil: Andrade, Bruno G. N.. Munster Technological University; Irlanda
Fil: Cuadrat, Rafael R. C.. Berlín Institute for Medical Systems Biology; Alemania
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description In a previous work, we demonstrated that nasally administered Dolosigranulum pigrum 040417 beneficially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 3 (TLR3) and improved protection against Respiratory Syncytial Virus (RSV) in mice. In this work, we aimed to evaluate the immunomodulatory effects of D. pigrum 040417 in human respiratory epithelial cells and the potential ability of this immunobiotic bacterium to in-crease the protection against Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The respiratory commensal bacterium D. pigrum 040417 differentially modulated the production of IFN-β, IL-6, CXCL8, CCL5 and CXCL10 in the culture supernatants of Calu-3 cells stimulated with poly(I:C) or challenged with SARS-CoV-2. The differential cytokine profile induced by the 040417 strain was associated with a significant reduction in viral replication and cellular damage after coronavirus infection. Of note, D. pigrum 030918 was not able to modify the resistance of Calu-3 cells to SARS-CoV-2 infection, indicating a strain-specific immunomodulatory effect for respiratory commensal bacteria. The findings of this work improve our understanding of the immunological mechanisms involved in the modulation of respiratory immunity induced by respiratory commensal bacteria, by demonstrating their specific effect on respiratory epithelial cells. In addition, the results suggest that particular strains such as D. pigrum 040417 could be used as a promising alternative for combating SARS-CoV-2 and reducing the severity of COVID-19.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153800
Islam, Md Aminul; Albarracín, Leonardo Miguel; Melnikov, Vyacheslav; Andrade, Bruno G. N.; Cuadrat, Rafael R. C.; et al.; Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells; MDPI AG; Pathogens; 10; 6; 5-2021; 1-11
2076-0817
2076-0817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153800
identifier_str_mv Islam, Md Aminul; Albarracín, Leonardo Miguel; Melnikov, Vyacheslav; Andrade, Bruno G. N.; Cuadrat, Rafael R. C.; et al.; Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells; MDPI AG; Pathogens; 10; 6; 5-2021; 1-11
2076-0817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10060634
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/6/634
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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