Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection

Autores
Islam, Md Aminul; Albarracín, Leonardo Miguel; Tomokiyo, Mikado; Valdéz, Juan Carlos; Sacur, Jacinto Alfredo; Vizoso Pinto, María Guadalupe; Andrade, Bruno G. N.; Cuadrat, Rafael R. C.; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previously, we reported that immunomodulatory lactobacilli, nasally administered, benefi-cially regulated the lung antiviral innate immune response induced by Toll-like receptor 3 (TLR3) activation and improved protection against the respiratory pathogens, influenza virus and respiratory syncytial virus in mice. Here, we assessed the immunomodulatory effects of viable and non-viable Lactiplantibacillus plantarum strains in human respiratory epithelial cells (Calu-3 cells) and the capacity of these immunobiotic lactobacilli to reduce their susceptibility to the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Immunobiotic L. plantarum MPL16 and CRL1506 differentially modulated IFN-β, IL-6, CXCL8, CCL5 and CXCL10 production and IFNAR2, DDX58, Mx1 and OAS1 expression in Calu-3 cells stimulated with the TLR3 agonist poly(I:C). Furthermore, the MPL16 and CRL1506 strains increased the resistance of Calu-3 cells to the challenge with SARS-CoV-2. L. plantarum MPL16 induced these beneficial effects more efficiently than the CRL1506 strain. Of note, neither non-viable MPL16 and CRL1506 strains nor the non-immunomodulatory strains L. plantarum CRL1905 and MPL18 could modify the resistance of Calu-3 cells to SARS-CoV-2 infection or the immune response to poly(I:C) challenge. To date, the potential beneficial effects of immunomodulatory probiotics on SARS-CoV-2 infection and COVID-19 outcome have been extrapolated from studies carried out in the context of other viral pathogens. To the best of our knowledge, this is the first demonstration of the ability of immunomodulatory lactobacilli to positively influence the replication of the new coronavirus. Further mechanistic studies and in vivo experiments in animal models of SARS-CoV-2 infection are necessary to identify specific strains of beneficial immunobiotic lactobacilli like L. plantarum MPL16 or CRL1506 for the prevention or treatment of the COVID-19.
Fil: Islam, Md Aminul. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán; Argentina
Fil: Sacur, Jacinto Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Andrade, Bruno G. N.. No especifíca;
Fil: Cuadrat, Rafael R. C.. No especifíca;
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
CORONAVIRUS
COVID-19
IMMUNOBIOTICS
LACTIPLANTIBACILLUS PLANTARUM
PROBIOTICS
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
TLR3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149763
Acceder
id CONICETDig_544824637b2114418cfe06fceed9eb89
oai_identifier_str oai:ri.conicet.gov.ar:11336/149763
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infectionIslam, Md AminulAlbarracín, Leonardo MiguelTomokiyo, MikadoValdéz, Juan CarlosSacur, Jacinto AlfredoVizoso Pinto, María GuadalupeAndrade, Bruno G. N.Cuadrat, Rafael R. C.Kitazawa, HarukiVillena, Julio CesarCORONAVIRUSCOVID-19IMMUNOBIOTICSLACTIPLANTIBACILLUS PLANTARUMPROBIOTICSRESPIRATORY EPITHELIAL CELLSSARS-COV-2TLR3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Previously, we reported that immunomodulatory lactobacilli, nasally administered, benefi-cially regulated the lung antiviral innate immune response induced by Toll-like receptor 3 (TLR3) activation and improved protection against the respiratory pathogens, influenza virus and respiratory syncytial virus in mice. Here, we assessed the immunomodulatory effects of viable and non-viable Lactiplantibacillus plantarum strains in human respiratory epithelial cells (Calu-3 cells) and the capacity of these immunobiotic lactobacilli to reduce their susceptibility to the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Immunobiotic L. plantarum MPL16 and CRL1506 differentially modulated IFN-β, IL-6, CXCL8, CCL5 and CXCL10 production and IFNAR2, DDX58, Mx1 and OAS1 expression in Calu-3 cells stimulated with the TLR3 agonist poly(I:C). Furthermore, the MPL16 and CRL1506 strains increased the resistance of Calu-3 cells to the challenge with SARS-CoV-2. L. plantarum MPL16 induced these beneficial effects more efficiently than the CRL1506 strain. Of note, neither non-viable MPL16 and CRL1506 strains nor the non-immunomodulatory strains L. plantarum CRL1905 and MPL18 could modify the resistance of Calu-3 cells to SARS-CoV-2 infection or the immune response to poly(I:C) challenge. To date, the potential beneficial effects of immunomodulatory probiotics on SARS-CoV-2 infection and COVID-19 outcome have been extrapolated from studies carried out in the context of other viral pathogens. To the best of our knowledge, this is the first demonstration of the ability of immunomodulatory lactobacilli to positively influence the replication of the new coronavirus. Further mechanistic studies and in vivo experiments in animal models of SARS-CoV-2 infection are necessary to identify specific strains of beneficial immunobiotic lactobacilli like L. plantarum MPL16 or CRL1506 for the prevention or treatment of the COVID-19.Fil: Islam, Md Aminul. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán; ArgentinaFil: Sacur, Jacinto Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Andrade, Bruno G. N.. No especifíca;Fil: Cuadrat, Rafael R. C.. No especifíca;Fil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMolecular Diversity Preservation International2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149763Islam, Md Aminul; Albarracín, Leonardo Miguel; Tomokiyo, Mikado; Valdéz, Juan Carlos; Sacur, Jacinto Alfredo; et al.; Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection; Molecular Diversity Preservation International; Pathogens; 10; 9; 9-2021; 1-162076-0817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/9/1197info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10091197info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:26:10Zoai:ri.conicet.gov.ar:11336/149763instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:26:11.054CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
title Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
spellingShingle Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
Islam, Md Aminul
CORONAVIRUS
COVID-19
IMMUNOBIOTICS
LACTIPLANTIBACILLUS PLANTARUM
PROBIOTICS
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
TLR3
title_short Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
title_full Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
title_fullStr Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
title_full_unstemmed Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
title_sort Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
dc.creator.none.fl_str_mv Islam, Md Aminul
Albarracín, Leonardo Miguel
Tomokiyo, Mikado
Valdéz, Juan Carlos
Sacur, Jacinto Alfredo
Vizoso Pinto, María Guadalupe
Andrade, Bruno G. N.
Cuadrat, Rafael R. C.
Kitazawa, Haruki
Villena, Julio Cesar
author Islam, Md Aminul
author_facet Islam, Md Aminul
Albarracín, Leonardo Miguel
Tomokiyo, Mikado
Valdéz, Juan Carlos
Sacur, Jacinto Alfredo
Vizoso Pinto, María Guadalupe
Andrade, Bruno G. N.
Cuadrat, Rafael R. C.
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Albarracín, Leonardo Miguel
Tomokiyo, Mikado
Valdéz, Juan Carlos
Sacur, Jacinto Alfredo
Vizoso Pinto, María Guadalupe
Andrade, Bruno G. N.
Cuadrat, Rafael R. C.
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CORONAVIRUS
COVID-19
IMMUNOBIOTICS
LACTIPLANTIBACILLUS PLANTARUM
PROBIOTICS
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
TLR3
topic CORONAVIRUS
COVID-19
IMMUNOBIOTICS
LACTIPLANTIBACILLUS PLANTARUM
PROBIOTICS
RESPIRATORY EPITHELIAL CELLS
SARS-COV-2
TLR3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Previously, we reported that immunomodulatory lactobacilli, nasally administered, benefi-cially regulated the lung antiviral innate immune response induced by Toll-like receptor 3 (TLR3) activation and improved protection against the respiratory pathogens, influenza virus and respiratory syncytial virus in mice. Here, we assessed the immunomodulatory effects of viable and non-viable Lactiplantibacillus plantarum strains in human respiratory epithelial cells (Calu-3 cells) and the capacity of these immunobiotic lactobacilli to reduce their susceptibility to the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Immunobiotic L. plantarum MPL16 and CRL1506 differentially modulated IFN-β, IL-6, CXCL8, CCL5 and CXCL10 production and IFNAR2, DDX58, Mx1 and OAS1 expression in Calu-3 cells stimulated with the TLR3 agonist poly(I:C). Furthermore, the MPL16 and CRL1506 strains increased the resistance of Calu-3 cells to the challenge with SARS-CoV-2. L. plantarum MPL16 induced these beneficial effects more efficiently than the CRL1506 strain. Of note, neither non-viable MPL16 and CRL1506 strains nor the non-immunomodulatory strains L. plantarum CRL1905 and MPL18 could modify the resistance of Calu-3 cells to SARS-CoV-2 infection or the immune response to poly(I:C) challenge. To date, the potential beneficial effects of immunomodulatory probiotics on SARS-CoV-2 infection and COVID-19 outcome have been extrapolated from studies carried out in the context of other viral pathogens. To the best of our knowledge, this is the first demonstration of the ability of immunomodulatory lactobacilli to positively influence the replication of the new coronavirus. Further mechanistic studies and in vivo experiments in animal models of SARS-CoV-2 infection are necessary to identify specific strains of beneficial immunobiotic lactobacilli like L. plantarum MPL16 or CRL1506 for the prevention or treatment of the COVID-19.
Fil: Islam, Md Aminul. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán; Argentina
Fil: Sacur, Jacinto Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Andrade, Bruno G. N.. No especifíca;
Fil: Cuadrat, Rafael R. C.. No especifíca;
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Previously, we reported that immunomodulatory lactobacilli, nasally administered, benefi-cially regulated the lung antiviral innate immune response induced by Toll-like receptor 3 (TLR3) activation and improved protection against the respiratory pathogens, influenza virus and respiratory syncytial virus in mice. Here, we assessed the immunomodulatory effects of viable and non-viable Lactiplantibacillus plantarum strains in human respiratory epithelial cells (Calu-3 cells) and the capacity of these immunobiotic lactobacilli to reduce their susceptibility to the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Immunobiotic L. plantarum MPL16 and CRL1506 differentially modulated IFN-β, IL-6, CXCL8, CCL5 and CXCL10 production and IFNAR2, DDX58, Mx1 and OAS1 expression in Calu-3 cells stimulated with the TLR3 agonist poly(I:C). Furthermore, the MPL16 and CRL1506 strains increased the resistance of Calu-3 cells to the challenge with SARS-CoV-2. L. plantarum MPL16 induced these beneficial effects more efficiently than the CRL1506 strain. Of note, neither non-viable MPL16 and CRL1506 strains nor the non-immunomodulatory strains L. plantarum CRL1905 and MPL18 could modify the resistance of Calu-3 cells to SARS-CoV-2 infection or the immune response to poly(I:C) challenge. To date, the potential beneficial effects of immunomodulatory probiotics on SARS-CoV-2 infection and COVID-19 outcome have been extrapolated from studies carried out in the context of other viral pathogens. To the best of our knowledge, this is the first demonstration of the ability of immunomodulatory lactobacilli to positively influence the replication of the new coronavirus. Further mechanistic studies and in vivo experiments in animal models of SARS-CoV-2 infection are necessary to identify specific strains of beneficial immunobiotic lactobacilli like L. plantarum MPL16 or CRL1506 for the prevention or treatment of the COVID-19.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149763
Islam, Md Aminul; Albarracín, Leonardo Miguel; Tomokiyo, Mikado; Valdéz, Juan Carlos; Sacur, Jacinto Alfredo; et al.; Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection; Molecular Diversity Preservation International; Pathogens; 10; 9; 9-2021; 1-16
2076-0817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149763
identifier_str_mv Islam, Md Aminul; Albarracín, Leonardo Miguel; Tomokiyo, Mikado; Valdéz, Juan Carlos; Sacur, Jacinto Alfredo; et al.; Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection; Molecular Diversity Preservation International; Pathogens; 10; 9; 9-2021; 1-16
2076-0817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/9/1197
info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10091197
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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