The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
- Autores
- Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; Kanmani, Paulraj; Melnikov, Vyacheslav; Kurata, Shoichiro; Kitazawa, Haruki; Villena, Julio Cesar
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Quilodrán Vega, Sandra. Universidad de Concepción; Chile
Fil: Yamamuro, Hikari. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Melnikov, Vyacheslav. Saint Petersburg State University; Rusia
Fil: Kurata, Shoichiro. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina - Materia
-
ALVEOLAR MACROPHAGES
DOLOSIGRANULUM PIGRUM
NEXT-GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
STREPTOCOCCUS PNEUMONIAE
UPPER RESPIRATORY TRACT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/184312
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniaeRaya Tonetti, María FernandaTomokiyo, MikadoOrtiz Moyano, Francisco RamiroQuilodrán Vega, SandraYamamuro, HikariKanmani, PaulrajMelnikov, VyacheslavKurata, ShoichiroKitazawa, HarukiVillena, Julio CesarALVEOLAR MACROPHAGESDOLOSIGRANULUM PIGRUMNEXT-GENERATION PROBIOTICSRESPIRATORY COMMENSAL BACTERIASTREPTOCOCCUS PNEUMONIAEUPPER RESPIRATORY TRACThttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Quilodrán Vega, Sandra. Universidad de Concepción; ChileFil: Yamamuro, Hikari. Tohoku University; JapónFil: Kanmani, Paulraj. Tohoku University; JapónFil: Melnikov, Vyacheslav. Saint Petersburg State University; RusiaFil: Kurata, Shoichiro. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMDPI AG2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184312Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; et al.; The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae; MDPI AG; Microorganisms; 9; 6; 6-2021; 1-192076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/6/1324info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9061324info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:27:28Zoai:ri.conicet.gov.ar:11336/184312instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:27:28.941CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae |
title |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae |
spellingShingle |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae Raya Tonetti, María Fernanda ALVEOLAR MACROPHAGES DOLOSIGRANULUM PIGRUM NEXT-GENERATION PROBIOTICS RESPIRATORY COMMENSAL BACTERIA STREPTOCOCCUS PNEUMONIAE UPPER RESPIRATORY TRACT |
title_short |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae |
title_full |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae |
title_fullStr |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae |
title_full_unstemmed |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae |
title_sort |
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae |
dc.creator.none.fl_str_mv |
Raya Tonetti, María Fernanda Tomokiyo, Mikado Ortiz Moyano, Francisco Ramiro Quilodrán Vega, Sandra Yamamuro, Hikari Kanmani, Paulraj Melnikov, Vyacheslav Kurata, Shoichiro Kitazawa, Haruki Villena, Julio Cesar |
author |
Raya Tonetti, María Fernanda |
author_facet |
Raya Tonetti, María Fernanda Tomokiyo, Mikado Ortiz Moyano, Francisco Ramiro Quilodrán Vega, Sandra Yamamuro, Hikari Kanmani, Paulraj Melnikov, Vyacheslav Kurata, Shoichiro Kitazawa, Haruki Villena, Julio Cesar |
author_role |
author |
author2 |
Tomokiyo, Mikado Ortiz Moyano, Francisco Ramiro Quilodrán Vega, Sandra Yamamuro, Hikari Kanmani, Paulraj Melnikov, Vyacheslav Kurata, Shoichiro Kitazawa, Haruki Villena, Julio Cesar |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
ALVEOLAR MACROPHAGES DOLOSIGRANULUM PIGRUM NEXT-GENERATION PROBIOTICS RESPIRATORY COMMENSAL BACTERIA STREPTOCOCCUS PNEUMONIAE UPPER RESPIRATORY TRACT |
topic |
ALVEOLAR MACROPHAGES DOLOSIGRANULUM PIGRUM NEXT-GENERATION PROBIOTICS RESPIRATORY COMMENSAL BACTERIA STREPTOCOCCUS PNEUMONIAE UPPER RESPIRATORY TRACT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics. Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Tomokiyo, Mikado. Tohoku University; Japón Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Quilodrán Vega, Sandra. Universidad de Concepción; Chile Fil: Yamamuro, Hikari. Tohoku University; Japón Fil: Kanmani, Paulraj. Tohoku University; Japón Fil: Melnikov, Vyacheslav. Saint Petersburg State University; Rusia Fil: Kurata, Shoichiro. Tohoku University; Japón Fil: Kitazawa, Haruki. Tohoku University; Japón Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina |
description |
Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/184312 Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; et al.; The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae; MDPI AG; Microorganisms; 9; 6; 6-2021; 1-19 2076-2607 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/184312 |
identifier_str_mv |
Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; et al.; The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae; MDPI AG; Microorganisms; 9; 6; 6-2021; 1-19 2076-2607 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/6/1324 info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9061324 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
MDPI AG |
publisher.none.fl_str_mv |
MDPI AG |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846082730548264960 |
score |
13.22299 |