The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae

Autores
Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; Kanmani, Paulraj; Melnikov, Vyacheslav; Kurata, Shoichiro; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Quilodrán Vega, Sandra. Universidad de Concepción; Chile
Fil: Yamamuro, Hikari. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Melnikov, Vyacheslav. Saint Petersburg State University; Rusia
Fil: Kurata, Shoichiro. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
ALVEOLAR MACROPHAGES
DOLOSIGRANULUM PIGRUM
NEXT-GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
STREPTOCOCCUS PNEUMONIAE
UPPER RESPIRATORY TRACT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184312

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oai_identifier_str oai:ri.conicet.gov.ar:11336/184312
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniaeRaya Tonetti, María FernandaTomokiyo, MikadoOrtiz Moyano, Francisco RamiroQuilodrán Vega, SandraYamamuro, HikariKanmani, PaulrajMelnikov, VyacheslavKurata, ShoichiroKitazawa, HarukiVillena, Julio CesarALVEOLAR MACROPHAGESDOLOSIGRANULUM PIGRUMNEXT-GENERATION PROBIOTICSRESPIRATORY COMMENSAL BACTERIASTREPTOCOCCUS PNEUMONIAEUPPER RESPIRATORY TRACThttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Quilodrán Vega, Sandra. Universidad de Concepción; ChileFil: Yamamuro, Hikari. Tohoku University; JapónFil: Kanmani, Paulraj. Tohoku University; JapónFil: Melnikov, Vyacheslav. Saint Petersburg State University; RusiaFil: Kurata, Shoichiro. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMDPI AG2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184312Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; et al.; The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae; MDPI AG; Microorganisms; 9; 6; 6-2021; 1-192076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/6/1324info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9061324info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:27:28Zoai:ri.conicet.gov.ar:11336/184312instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:27:28.941CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
spellingShingle The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
Raya Tonetti, María Fernanda
ALVEOLAR MACROPHAGES
DOLOSIGRANULUM PIGRUM
NEXT-GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
STREPTOCOCCUS PNEUMONIAE
UPPER RESPIRATORY TRACT
title_short The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_full The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_fullStr The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_full_unstemmed The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_sort The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
dc.creator.none.fl_str_mv Raya Tonetti, María Fernanda
Tomokiyo, Mikado
Ortiz Moyano, Francisco Ramiro
Quilodrán Vega, Sandra
Yamamuro, Hikari
Kanmani, Paulraj
Melnikov, Vyacheslav
Kurata, Shoichiro
Kitazawa, Haruki
Villena, Julio Cesar
author Raya Tonetti, María Fernanda
author_facet Raya Tonetti, María Fernanda
Tomokiyo, Mikado
Ortiz Moyano, Francisco Ramiro
Quilodrán Vega, Sandra
Yamamuro, Hikari
Kanmani, Paulraj
Melnikov, Vyacheslav
Kurata, Shoichiro
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Tomokiyo, Mikado
Ortiz Moyano, Francisco Ramiro
Quilodrán Vega, Sandra
Yamamuro, Hikari
Kanmani, Paulraj
Melnikov, Vyacheslav
Kurata, Shoichiro
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALVEOLAR MACROPHAGES
DOLOSIGRANULUM PIGRUM
NEXT-GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
STREPTOCOCCUS PNEUMONIAE
UPPER RESPIRATORY TRACT
topic ALVEOLAR MACROPHAGES
DOLOSIGRANULUM PIGRUM
NEXT-GENERATION PROBIOTICS
RESPIRATORY COMMENSAL BACTERIA
STREPTOCOCCUS PNEUMONIAE
UPPER RESPIRATORY TRACT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Quilodrán Vega, Sandra. Universidad de Concepción; Chile
Fil: Yamamuro, Hikari. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Melnikov, Vyacheslav. Saint Petersburg State University; Rusia
Fil: Kurata, Shoichiro. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Tolllike receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184312
Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; et al.; The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae; MDPI AG; Microorganisms; 9; 6; 6-2021; 1-19
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184312
identifier_str_mv Raya Tonetti, María Fernanda; Tomokiyo, Mikado; Ortiz Moyano, Francisco Ramiro; Quilodrán Vega, Sandra; Yamamuro, Hikari; et al.; The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae; MDPI AG; Microorganisms; 9; 6; 6-2021; 1-19
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/6/1324
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9061324
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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