Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection

Autores
Raya Tonetti, María Fernanda; Ortiz Moyano, Francisco Ramiro; Tomokiyo, Mikado; Melnikov, Vyacheslav; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Previously, we demonstrated that the nasal administration of the respiratory commensal bacterium Dolosigranulum pigrum 040417 (Dp04) to infant mice differentially modulates the respiratory immune response triggered by Toll-like receptor (TLR)-2 activation, increasing the resistance to Streptococcus pneumoniae (Sp) infection. The nasal priming with Dp04 reduced pneumococcal counts in lung and blood and diminished the levels of lung injury biomarkers. In this work, we aimed to characterize the role of alveolar macrophages (AM) on the immunomodulatory properties of Dp04 in the context of pneumococcal infection. In the first set of experiments, mice were nasally stimulated with Dp04 (108 cells/mouse/day) for 5 consecutive days and then challenged with 106 CFU of Sp. Variations in numbers and functionality of resident AM in broncho-alveolar lavage (BAL) samples were evaluated. The number of activated CD11c+SiglecF+MHC-IIhi AM was significantly increased after Sp challenge in mice primed with Dp04 than in controls (p<0.05). Furthermore, AM obtained from Dp04-treated mice produced in vitro higher levels of IFN-β and IFN-γ, as well as IL-10 and IL-27 compared to the control group (p<0.05). In a second set of experiments, AM were depleted using liposomes containing clodronate (CLP) before the stimulation of with Dp04. The CLP treatment significantly affected the ability of Dp04 to reduce pneumococcal cell counts, as well as lung injury biomarkers. In addition, AM depletion impaired the capacity of Dp04 to differentially modulate the cytokine profile in the respiratory tract. The ability of Dp04 to increase the levels of BAL IFN-γ, IL-10 and IL-27 in response to Sp infection was abolished when AM were depleted by CLP (p<0.05). This results show for the first time that AM have a relevant role in the immunomodulatory effect of Dp04. Our results also mark a significant advance in the positioning of Dp04 as a next-generation probiotic for the respiratory tract.
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute of Epidemiology and Microbiology; Rusia
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Materia
ALVEOLAR MACROPHAGES
CLODRONATE LIPOSOMES
RESPIRATORY COMMENSAL BACTERIA
INNATE RESPIRATORY IMMUNITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158438
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/158438
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infectionRaya Tonetti, María FernandaOrtiz Moyano, Francisco RamiroTomokiyo, MikadoMelnikov, VyacheslavKitazawa, HarukiVillena, Julio CesarALVEOLAR MACROPHAGESCLODRONATE LIPOSOMESRESPIRATORY COMMENSAL BACTERIAINNATE RESPIRATORY IMMUNITYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Previously, we demonstrated that the nasal administration of the respiratory commensal bacterium Dolosigranulum pigrum 040417 (Dp04) to infant mice differentially modulates the respiratory immune response triggered by Toll-like receptor (TLR)-2 activation, increasing the resistance to Streptococcus pneumoniae (Sp) infection. The nasal priming with Dp04 reduced pneumococcal counts in lung and blood and diminished the levels of lung injury biomarkers. In this work, we aimed to characterize the role of alveolar macrophages (AM) on the immunomodulatory properties of Dp04 in the context of pneumococcal infection. In the first set of experiments, mice were nasally stimulated with Dp04 (108 cells/mouse/day) for 5 consecutive days and then challenged with 106 CFU of Sp. Variations in numbers and functionality of resident AM in broncho-alveolar lavage (BAL) samples were evaluated. The number of activated CD11c+SiglecF+MHC-IIhi AM was significantly increased after Sp challenge in mice primed with Dp04 than in controls (p<0.05). Furthermore, AM obtained from Dp04-treated mice produced in vitro higher levels of IFN-β and IFN-γ, as well as IL-10 and IL-27 compared to the control group (p<0.05). In a second set of experiments, AM were depleted using liposomes containing clodronate (CLP) before the stimulation of with Dp04. The CLP treatment significantly affected the ability of Dp04 to reduce pneumococcal cell counts, as well as lung injury biomarkers. In addition, AM depletion impaired the capacity of Dp04 to differentially modulate the cytokine profile in the respiratory tract. The ability of Dp04 to increase the levels of BAL IFN-γ, IL-10 and IL-27 in response to Sp infection was abolished when AM were depleted by CLP (p<0.05). This results show for the first time that AM have a relevant role in the immunomodulatory effect of Dp04. Our results also mark a significant advance in the positioning of Dp04 as a next-generation probiotic for the respiratory tract.Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Melnikov, Vyacheslav. Gabrichevsky Research Institute of Epidemiology and Microbiology; RusiaFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologiaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/158438Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 142-1420025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:44Zoai:ri.conicet.gov.ar:11336/158438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:44.362CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
title Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
spellingShingle Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
Raya Tonetti, María Fernanda
ALVEOLAR MACROPHAGES
CLODRONATE LIPOSOMES
RESPIRATORY COMMENSAL BACTERIA
INNATE RESPIRATORY IMMUNITY
title_short Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
title_full Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
title_fullStr Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
title_full_unstemmed Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
title_sort Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection
dc.creator.none.fl_str_mv Raya Tonetti, María Fernanda
Ortiz Moyano, Francisco Ramiro
Tomokiyo, Mikado
Melnikov, Vyacheslav
Kitazawa, Haruki
Villena, Julio Cesar
author Raya Tonetti, María Fernanda
author_facet Raya Tonetti, María Fernanda
Ortiz Moyano, Francisco Ramiro
Tomokiyo, Mikado
Melnikov, Vyacheslav
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Ortiz Moyano, Francisco Ramiro
Tomokiyo, Mikado
Melnikov, Vyacheslav
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALVEOLAR MACROPHAGES
CLODRONATE LIPOSOMES
RESPIRATORY COMMENSAL BACTERIA
INNATE RESPIRATORY IMMUNITY
topic ALVEOLAR MACROPHAGES
CLODRONATE LIPOSOMES
RESPIRATORY COMMENSAL BACTERIA
INNATE RESPIRATORY IMMUNITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previously, we demonstrated that the nasal administration of the respiratory commensal bacterium Dolosigranulum pigrum 040417 (Dp04) to infant mice differentially modulates the respiratory immune response triggered by Toll-like receptor (TLR)-2 activation, increasing the resistance to Streptococcus pneumoniae (Sp) infection. The nasal priming with Dp04 reduced pneumococcal counts in lung and blood and diminished the levels of lung injury biomarkers. In this work, we aimed to characterize the role of alveolar macrophages (AM) on the immunomodulatory properties of Dp04 in the context of pneumococcal infection. In the first set of experiments, mice were nasally stimulated with Dp04 (108 cells/mouse/day) for 5 consecutive days and then challenged with 106 CFU of Sp. Variations in numbers and functionality of resident AM in broncho-alveolar lavage (BAL) samples were evaluated. The number of activated CD11c+SiglecF+MHC-IIhi AM was significantly increased after Sp challenge in mice primed with Dp04 than in controls (p<0.05). Furthermore, AM obtained from Dp04-treated mice produced in vitro higher levels of IFN-β and IFN-γ, as well as IL-10 and IL-27 compared to the control group (p<0.05). In a second set of experiments, AM were depleted using liposomes containing clodronate (CLP) before the stimulation of with Dp04. The CLP treatment significantly affected the ability of Dp04 to reduce pneumococcal cell counts, as well as lung injury biomarkers. In addition, AM depletion impaired the capacity of Dp04 to differentially modulate the cytokine profile in the respiratory tract. The ability of Dp04 to increase the levels of BAL IFN-γ, IL-10 and IL-27 in response to Sp infection was abolished when AM were depleted by CLP (p<0.05). This results show for the first time that AM have a relevant role in the immunomodulatory effect of Dp04. Our results also mark a significant advance in the positioning of Dp04 as a next-generation probiotic for the respiratory tract.
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute of Epidemiology and Microbiology; Rusia
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
description Previously, we demonstrated that the nasal administration of the respiratory commensal bacterium Dolosigranulum pigrum 040417 (Dp04) to infant mice differentially modulates the respiratory immune response triggered by Toll-like receptor (TLR)-2 activation, increasing the resistance to Streptococcus pneumoniae (Sp) infection. The nasal priming with Dp04 reduced pneumococcal counts in lung and blood and diminished the levels of lung injury biomarkers. In this work, we aimed to characterize the role of alveolar macrophages (AM) on the immunomodulatory properties of Dp04 in the context of pneumococcal infection. In the first set of experiments, mice were nasally stimulated with Dp04 (108 cells/mouse/day) for 5 consecutive days and then challenged with 106 CFU of Sp. Variations in numbers and functionality of resident AM in broncho-alveolar lavage (BAL) samples were evaluated. The number of activated CD11c+SiglecF+MHC-IIhi AM was significantly increased after Sp challenge in mice primed with Dp04 than in controls (p<0.05). Furthermore, AM obtained from Dp04-treated mice produced in vitro higher levels of IFN-β and IFN-γ, as well as IL-10 and IL-27 compared to the control group (p<0.05). In a second set of experiments, AM were depleted using liposomes containing clodronate (CLP) before the stimulation of with Dp04. The CLP treatment significantly affected the ability of Dp04 to reduce pneumococcal cell counts, as well as lung injury biomarkers. In addition, AM depletion impaired the capacity of Dp04 to differentially modulate the cytokine profile in the respiratory tract. The ability of Dp04 to increase the levels of BAL IFN-γ, IL-10 and IL-27 in response to Sp infection was abolished when AM were depleted by CLP (p<0.05). This results show for the first time that AM have a relevant role in the immunomodulatory effect of Dp04. Our results also mark a significant advance in the positioning of Dp04 as a next-generation probiotic for the respiratory tract.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158438
Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 142-142
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158438
identifier_str_mv Alveolar macrophages depletion affects the ability of Dolosigranulum pigrum 040417 to protect infant mice against pneumococcal infection; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión anual de la Sociedad Argentina de Immunología (SAI); LIII Reunión anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 142-142
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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