Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium

Autores
Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; Zhou, Binghui; Ikeda Ohtsubo, Wakako; Nishiyama, Keita; Aso, Hisashi; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses.
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Zhuang, Tao. Tohoku University; Japón
Fil: Toyoshi, Eita. Tohoku University; Japón
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saha, Sudeb. Tohoku University; Japón
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
RESPIRATORY EPITHELIUM
EPITHELIAL CELLS
TLR3
VIRAL IMMUNITY
TLR4
PORCINE RESPIRATORY EPITHELIAL CELL LINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/249546

id CONICETDig_c83342a33a12ca2cbcd2741494c240c1
oai_identifier_str oai:ri.conicet.gov.ar:11336/249546
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epitheliumFukuyama, KohtaroZhuang, TaoToyoshi, EitaRaya Tonetti, María FernandaSaha, SudebZhou, BinghuiIkeda Ohtsubo, WakakoNishiyama, KeitaAso, HisashiVillena, Julio CesarKitazawa, HarukiRESPIRATORY EPITHELIUMEPITHELIAL CELLSTLR3VIRAL IMMUNITYTLR4PORCINE RESPIRATORY EPITHELIAL CELL LINEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses.Fil: Fukuyama, Kohtaro. Tohoku University; JapónFil: Zhuang, Tao. Tohoku University; JapónFil: Toyoshi, Eita. Tohoku University; JapónFil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Saha, Sudeb. Tohoku University; JapónFil: Zhou, Binghui. Tohoku University; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Nishiyama, Keita. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónFrontiers Media2023-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/249546Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; et al.; Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium; Frontiers Media; Frontiers in Immunology; 14; 3-7-2023; 1-141664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2023.1117102/info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2023.1117102info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:44:08Zoai:ri.conicet.gov.ar:11336/249546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:44:08.608CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
title Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
spellingShingle Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
Fukuyama, Kohtaro
RESPIRATORY EPITHELIUM
EPITHELIAL CELLS
TLR3
VIRAL IMMUNITY
TLR4
PORCINE RESPIRATORY EPITHELIAL CELL LINE
title_short Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
title_full Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
title_fullStr Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
title_full_unstemmed Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
title_sort Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
dc.creator.none.fl_str_mv Fukuyama, Kohtaro
Zhuang, Tao
Toyoshi, Eita
Raya Tonetti, María Fernanda
Saha, Sudeb
Zhou, Binghui
Ikeda Ohtsubo, Wakako
Nishiyama, Keita
Aso, Hisashi
Villena, Julio Cesar
Kitazawa, Haruki
author Fukuyama, Kohtaro
author_facet Fukuyama, Kohtaro
Zhuang, Tao
Toyoshi, Eita
Raya Tonetti, María Fernanda
Saha, Sudeb
Zhou, Binghui
Ikeda Ohtsubo, Wakako
Nishiyama, Keita
Aso, Hisashi
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Zhuang, Tao
Toyoshi, Eita
Raya Tonetti, María Fernanda
Saha, Sudeb
Zhou, Binghui
Ikeda Ohtsubo, Wakako
Nishiyama, Keita
Aso, Hisashi
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RESPIRATORY EPITHELIUM
EPITHELIAL CELLS
TLR3
VIRAL IMMUNITY
TLR4
PORCINE RESPIRATORY EPITHELIAL CELL LINE
topic RESPIRATORY EPITHELIUM
EPITHELIAL CELLS
TLR3
VIRAL IMMUNITY
TLR4
PORCINE RESPIRATORY EPITHELIAL CELL LINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses.
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Zhuang, Tao. Tohoku University; Japón
Fil: Toyoshi, Eita. Tohoku University; Japón
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saha, Sudeb. Tohoku University; Japón
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
description In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/249546
Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; et al.; Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium; Frontiers Media; Frontiers in Immunology; 14; 3-7-2023; 1-14
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/249546
identifier_str_mv Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; et al.; Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium; Frontiers Media; Frontiers in Immunology; 14; 3-7-2023; 1-14
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2023.1117102/
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2023.1117102
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082950851985408
score 13.22299