Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium
- Autores
- Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; Zhou, Binghui; Ikeda Ohtsubo, Wakako; Nishiyama, Keita; Aso, Hisashi; Villena, Julio Cesar; Kitazawa, Haruki
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses.
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Zhuang, Tao. Tohoku University; Japón
Fil: Toyoshi, Eita. Tohoku University; Japón
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saha, Sudeb. Tohoku University; Japón
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón - Materia
-
RESPIRATORY EPITHELIUM
EPITHELIAL CELLS
TLR3
VIRAL IMMUNITY
TLR4
PORCINE RESPIRATORY EPITHELIAL CELL LINE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/249546
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epitheliumFukuyama, KohtaroZhuang, TaoToyoshi, EitaRaya Tonetti, María FernandaSaha, SudebZhou, BinghuiIkeda Ohtsubo, WakakoNishiyama, KeitaAso, HisashiVillena, Julio CesarKitazawa, HarukiRESPIRATORY EPITHELIUMEPITHELIAL CELLSTLR3VIRAL IMMUNITYTLR4PORCINE RESPIRATORY EPITHELIAL CELL LINEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses.Fil: Fukuyama, Kohtaro. Tohoku University; JapónFil: Zhuang, Tao. Tohoku University; JapónFil: Toyoshi, Eita. Tohoku University; JapónFil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Saha, Sudeb. Tohoku University; JapónFil: Zhou, Binghui. Tohoku University; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Nishiyama, Keita. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónFrontiers Media2023-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/249546Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; et al.; Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium; Frontiers Media; Frontiers in Immunology; 14; 3-7-2023; 1-141664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2023.1117102/info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2023.1117102info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:44:08Zoai:ri.conicet.gov.ar:11336/249546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:44:08.608CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium |
title |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium |
spellingShingle |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium Fukuyama, Kohtaro RESPIRATORY EPITHELIUM EPITHELIAL CELLS TLR3 VIRAL IMMUNITY TLR4 PORCINE RESPIRATORY EPITHELIAL CELL LINE |
title_short |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium |
title_full |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium |
title_fullStr |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium |
title_full_unstemmed |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium |
title_sort |
Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium |
dc.creator.none.fl_str_mv |
Fukuyama, Kohtaro Zhuang, Tao Toyoshi, Eita Raya Tonetti, María Fernanda Saha, Sudeb Zhou, Binghui Ikeda Ohtsubo, Wakako Nishiyama, Keita Aso, Hisashi Villena, Julio Cesar Kitazawa, Haruki |
author |
Fukuyama, Kohtaro |
author_facet |
Fukuyama, Kohtaro Zhuang, Tao Toyoshi, Eita Raya Tonetti, María Fernanda Saha, Sudeb Zhou, Binghui Ikeda Ohtsubo, Wakako Nishiyama, Keita Aso, Hisashi Villena, Julio Cesar Kitazawa, Haruki |
author_role |
author |
author2 |
Zhuang, Tao Toyoshi, Eita Raya Tonetti, María Fernanda Saha, Sudeb Zhou, Binghui Ikeda Ohtsubo, Wakako Nishiyama, Keita Aso, Hisashi Villena, Julio Cesar Kitazawa, Haruki |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
RESPIRATORY EPITHELIUM EPITHELIAL CELLS TLR3 VIRAL IMMUNITY TLR4 PORCINE RESPIRATORY EPITHELIAL CELL LINE |
topic |
RESPIRATORY EPITHELIUM EPITHELIAL CELLS TLR3 VIRAL IMMUNITY TLR4 PORCINE RESPIRATORY EPITHELIAL CELL LINE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses. Fil: Fukuyama, Kohtaro. Tohoku University; Japón Fil: Zhuang, Tao. Tohoku University; Japón Fil: Toyoshi, Eita. Tohoku University; Japón Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Saha, Sudeb. Tohoku University; Japón Fil: Zhou, Binghui. Tohoku University; Japón Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón Fil: Nishiyama, Keita. Tohoku University; Japón Fil: Aso, Hisashi. Tohoku University; Japón Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Kitazawa, Haruki. Tohoku University; Japón |
description |
In vitro culture models that precisely mirror the porcine respiratory epitheliumare needed to gain insight into how pathogens and host interact. In this study, anew porcine bronchial epithelial cell line, designated as PBE cells, wasestablished from the respiratory tract of a neonatal pig. PBE cells assumed acobblestone-epithelial like morphology with close contacts between the cellswhen they reached confluence. The PBE cell line was characterized in terms ofits expression of pattern recognition receptors (PRRs) and its ability to respond tothe activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways,which are key PRRs involved in the defense of the respiratory epithelium againstpathogens. PBE cells stimulated with poly(I:C) were able to up-regulate theexpression of IFN-b, IFN-l1 (IL-29), IFN-l3 (IL-28B), the antiviral factors Mx1,OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kineticsstudies of immune factors in PBE cells allow us to speculate that this cell line canbe a useful in vitro tool to investigate treatments that help to potentiate antiviralimmunity in the respiratory epithelium of the porcine host. In addition, poly(I:C)and LPS treatments increased the expression of the inflammatory cytokines TNFa, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression ofnegative regulators of the TLR signaling pathways. Then, PBE cells may also allowthe evaluation of treatments that can regulate TLR3- and TLR4-mediatedinflammatory injury in the porcine airway, thereby protecting the host againstharmful overresponses. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/249546 Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; et al.; Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium; Frontiers Media; Frontiers in Immunology; 14; 3-7-2023; 1-14 1664-3224 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/249546 |
identifier_str_mv |
Fukuyama, Kohtaro; Zhuang, Tao; Toyoshi, Eita; Raya Tonetti, María Fernanda; Saha, Sudeb; et al.; Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium; Frontiers Media; Frontiers in Immunology; 14; 3-7-2023; 1-14 1664-3224 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2023.1117102/ info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2023.1117102 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846082950851985408 |
score |
13.22299 |