Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool

Autores
Missana, Liliana Raquel; Jammal, María Victoria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recombinant Human Parathyroid Hormone (rhPTH 1-34) administration is an effective treatment to improve bone mass in osteoporosis. The aim of this study was to develop a Tissue Engeenering Tool for bone regeneration. We evaluated the efficacy of a freeze dried rhPTH membrane in calvarial critical size defect (CZD). 44 Wistar female rats (body weight 150 ± 50 g) with CZD (5mm) were divided into 4 groups: group 1: rhPTH membrane (rhPTHm); group 2: atelocollagen membrane (Cm); group 3: rhPTH and atelocollagen I (CrhPTHm); group 4: without any treatment (CG). All samples were evaluated on the 1st, 3rd and 6th weeks (wks) post-surgery by soft X-ray, histological and histometric studies. Soft X-ray results showed a radiolucent image with many irregular radiopaque areas. Histologically, rhPTHm was replaced by reticular bone (7%) since 3rd wk, and lamellar bone ossicles (30%) at 6th wk. Cm showed bone formation like composite bone type on wk 1st, 3rd and 6th (2%, 44% and 41% respectively). With CrhPTHm, bone formation was observed in all periods (2.4%, 48% and 53%), showing statistical difference with CG in the 3rd and 6th wks (p= 0.03 and 0.01). Our results demonstrated the effectiveness of a new biomaterial called CrhPTHm because its ability to regenerate calvarial CZD. Moreover, the membrane represents a new local intermittent delivery system allowing rhPTH slow release.
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
BONE REGENERATION
CRITICAL SIZE DEFECT
RHPTH
TYPE I COLLAGEN
GRAFT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102696
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102696
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering toolMissana, Liliana RaquelJammal, María VictoriaBONE REGENERATIONCRITICAL SIZE DEFECTRHPTHTYPE I COLLAGENGRAFThttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Recombinant Human Parathyroid Hormone (rhPTH 1-34) administration is an effective treatment to improve bone mass in osteoporosis. The aim of this study was to develop a Tissue Engeenering Tool for bone regeneration. We evaluated the efficacy of a freeze dried rhPTH membrane in calvarial critical size defect (CZD). 44 Wistar female rats (body weight 150 ± 50 g) with CZD (5mm) were divided into 4 groups: group 1: rhPTH membrane (rhPTHm); group 2: atelocollagen membrane (Cm); group 3: rhPTH and atelocollagen I (CrhPTHm); group 4: without any treatment (CG). All samples were evaluated on the 1st, 3rd and 6th weeks (wks) post-surgery by soft X-ray, histological and histometric studies. Soft X-ray results showed a radiolucent image with many irregular radiopaque areas. Histologically, rhPTHm was replaced by reticular bone (7%) since 3rd wk, and lamellar bone ossicles (30%) at 6th wk. Cm showed bone formation like composite bone type on wk 1st, 3rd and 6th (2%, 44% and 41% respectively). With CrhPTHm, bone formation was observed in all periods (2.4%, 48% and 53%), showing statistical difference with CG in the 3rd and 6th wks (p= 0.03 and 0.01). Our results demonstrated the effectiveness of a new biomaterial called CrhPTHm because its ability to regenerate calvarial CZD. Moreover, the membrane represents a new local intermittent delivery system allowing rhPTH slow release.Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaWiley2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102696Missana, Liliana Raquel; Jammal, María Victoria; Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool; Wiley; Journal of Biomedical Materials Research Part A; 102; 12; 2-2014; 4358-43641552-4965CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.35114info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.35114info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:45Zoai:ri.conicet.gov.ar:11336/102696instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:45.864CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
title Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
spellingShingle Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
Missana, Liliana Raquel
BONE REGENERATION
CRITICAL SIZE DEFECT
RHPTH
TYPE I COLLAGEN
GRAFT
title_short Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
title_full Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
title_fullStr Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
title_full_unstemmed Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
title_sort Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool
dc.creator.none.fl_str_mv Missana, Liliana Raquel
Jammal, María Victoria
author Missana, Liliana Raquel
author_facet Missana, Liliana Raquel
Jammal, María Victoria
author_role author
author2 Jammal, María Victoria
author2_role author
dc.subject.none.fl_str_mv BONE REGENERATION
CRITICAL SIZE DEFECT
RHPTH
TYPE I COLLAGEN
GRAFT
topic BONE REGENERATION
CRITICAL SIZE DEFECT
RHPTH
TYPE I COLLAGEN
GRAFT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Recombinant Human Parathyroid Hormone (rhPTH 1-34) administration is an effective treatment to improve bone mass in osteoporosis. The aim of this study was to develop a Tissue Engeenering Tool for bone regeneration. We evaluated the efficacy of a freeze dried rhPTH membrane in calvarial critical size defect (CZD). 44 Wistar female rats (body weight 150 ± 50 g) with CZD (5mm) were divided into 4 groups: group 1: rhPTH membrane (rhPTHm); group 2: atelocollagen membrane (Cm); group 3: rhPTH and atelocollagen I (CrhPTHm); group 4: without any treatment (CG). All samples were evaluated on the 1st, 3rd and 6th weeks (wks) post-surgery by soft X-ray, histological and histometric studies. Soft X-ray results showed a radiolucent image with many irregular radiopaque areas. Histologically, rhPTHm was replaced by reticular bone (7%) since 3rd wk, and lamellar bone ossicles (30%) at 6th wk. Cm showed bone formation like composite bone type on wk 1st, 3rd and 6th (2%, 44% and 41% respectively). With CrhPTHm, bone formation was observed in all periods (2.4%, 48% and 53%), showing statistical difference with CG in the 3rd and 6th wks (p= 0.03 and 0.01). Our results demonstrated the effectiveness of a new biomaterial called CrhPTHm because its ability to regenerate calvarial CZD. Moreover, the membrane represents a new local intermittent delivery system allowing rhPTH slow release.
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Recombinant Human Parathyroid Hormone (rhPTH 1-34) administration is an effective treatment to improve bone mass in osteoporosis. The aim of this study was to develop a Tissue Engeenering Tool for bone regeneration. We evaluated the efficacy of a freeze dried rhPTH membrane in calvarial critical size defect (CZD). 44 Wistar female rats (body weight 150 ± 50 g) with CZD (5mm) were divided into 4 groups: group 1: rhPTH membrane (rhPTHm); group 2: atelocollagen membrane (Cm); group 3: rhPTH and atelocollagen I (CrhPTHm); group 4: without any treatment (CG). All samples were evaluated on the 1st, 3rd and 6th weeks (wks) post-surgery by soft X-ray, histological and histometric studies. Soft X-ray results showed a radiolucent image with many irregular radiopaque areas. Histologically, rhPTHm was replaced by reticular bone (7%) since 3rd wk, and lamellar bone ossicles (30%) at 6th wk. Cm showed bone formation like composite bone type on wk 1st, 3rd and 6th (2%, 44% and 41% respectively). With CrhPTHm, bone formation was observed in all periods (2.4%, 48% and 53%), showing statistical difference with CG in the 3rd and 6th wks (p= 0.03 and 0.01). Our results demonstrated the effectiveness of a new biomaterial called CrhPTHm because its ability to regenerate calvarial CZD. Moreover, the membrane represents a new local intermittent delivery system allowing rhPTH slow release.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102696
Missana, Liliana Raquel; Jammal, María Victoria; Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool; Wiley; Journal of Biomedical Materials Research Part A; 102; 12; 2-2014; 4358-4364
1552-4965
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102696
identifier_str_mv Missana, Liliana Raquel; Jammal, María Victoria; Critical size defect regeneration by rhPTH collagen membrane as a new tissue engineering tool; Wiley; Journal of Biomedical Materials Research Part A; 102; 12; 2-2014; 4358-4364
1552-4965
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.35114
info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.35114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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