Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH

Autores
Jammal, María Victoria; Pastorino, Nina Fiorella; Abate, Carlos Mauricio; Takagi, Shin; Nagatsuka, Hotoshi; Missana, Liliana Raquel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intermittent Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective treatment for improving bone mass in patients with osteoporosis; however, its effects on bone regeneration are still unclear. The objective of this study was to evaluate the potential toxicity systemic rhPTH, as well as its ability to regenerate critical-sized defects (CZD) in bone. We used 43 female Wistar rats (body weight, 150 ± 50 g). Critical-sized bone defects in rat calvaria received vehicle alone (Control Group, CG) or daily rhPTH (20 ìg/Kg/day) by subcutaneous injection (Experimental Group, EG). We evaluated bone healing obtained at the 1st, 3rd, and 6th wks post-surgery by biochemical, soft x-ray, histological, and morphometric studies. In the EG, at the 1st and 3rd wks, many areas of focal osteoblast hyperplasia were found on parietal bone. At the 3rd wk, woven and/or lamellar bone, in an organized interconnected trabecular network, showed disrupted mineralization. At the 6th wk, looped bone was found to have formed patterns on parietal bone. New bone formed in the EG showed significant statistical differences (p = 0.023) at the 6th wk. Systemic rhPTH at the dose of 20 ìg/Kg/day was able to stimulate bone formation on rat CZD. Also, pre-existing and new bone showed non-proliferative forms of bone hyperostosis (increased non-neoplastic bone).
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Takagi, Shin. Okayama University; Japón
Fil: Nagatsuka, Hotoshi. Okayama University; Japón
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Materia
BONE CALVARIAL
CRITICAL DEFECT
PTH
RATS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243519
Acceder
id CONICETDig_235abfebb075384e28a75978535adda2
oai_identifier_str oai:ri.conicet.gov.ar:11336/243519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTHJammal, María VictoriaPastorino, Nina FiorellaAbate, Carlos MauricioTakagi, ShinNagatsuka, HotoshiMissana, Liliana RaquelBONE CALVARIALCRITICAL DEFECTPTHRATShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Intermittent Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective treatment for improving bone mass in patients with osteoporosis; however, its effects on bone regeneration are still unclear. The objective of this study was to evaluate the potential toxicity systemic rhPTH, as well as its ability to regenerate critical-sized defects (CZD) in bone. We used 43 female Wistar rats (body weight, 150 ± 50 g). Critical-sized bone defects in rat calvaria received vehicle alone (Control Group, CG) or daily rhPTH (20 ìg/Kg/day) by subcutaneous injection (Experimental Group, EG). We evaluated bone healing obtained at the 1st, 3rd, and 6th wks post-surgery by biochemical, soft x-ray, histological, and morphometric studies. In the EG, at the 1st and 3rd wks, many areas of focal osteoblast hyperplasia were found on parietal bone. At the 3rd wk, woven and/or lamellar bone, in an organized interconnected trabecular network, showed disrupted mineralization. At the 6th wk, looped bone was found to have formed patterns on parietal bone. New bone formed in the EG showed significant statistical differences (p = 0.023) at the 6th wk. Systemic rhPTH at the dose of 20 ìg/Kg/day was able to stimulate bone formation on rat CZD. Also, pre-existing and new bone showed non-proliferative forms of bone hyperostosis (increased non-neoplastic bone).Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Takagi, Shin. Okayama University; JapónFil: Nagatsuka, Hotoshi. Okayama University; JapónFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaThe Society for Hard Tissue Regenerative Biology2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243519Jammal, María Victoria; Pastorino, Nina Fiorella; Abate, Carlos Mauricio; Takagi, Shin; Nagatsuka, Hotoshi; et al.; Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH; The Society for Hard Tissue Regenerative Biology; Journal of Hard Tissue Biology; 21; 4; 7-2012; 443-4501341-7649CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/jhtb/21/4/21_443/_articleinfo:eu-repo/semantics/altIdentifier/doi/10.2485/jhtb.21.443info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:13:24Zoai:ri.conicet.gov.ar:11336/243519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:13:24.631CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
title Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
spellingShingle Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
Jammal, María Victoria
BONE CALVARIAL
CRITICAL DEFECT
PTH
RATS
title_short Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
title_full Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
title_fullStr Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
title_full_unstemmed Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
title_sort Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH
dc.creator.none.fl_str_mv Jammal, María Victoria
Pastorino, Nina Fiorella
Abate, Carlos Mauricio
Takagi, Shin
Nagatsuka, Hotoshi
Missana, Liliana Raquel
author Jammal, María Victoria
author_facet Jammal, María Victoria
Pastorino, Nina Fiorella
Abate, Carlos Mauricio
Takagi, Shin
Nagatsuka, Hotoshi
Missana, Liliana Raquel
author_role author
author2 Pastorino, Nina Fiorella
Abate, Carlos Mauricio
Takagi, Shin
Nagatsuka, Hotoshi
Missana, Liliana Raquel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BONE CALVARIAL
CRITICAL DEFECT
PTH
RATS
topic BONE CALVARIAL
CRITICAL DEFECT
PTH
RATS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Intermittent Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective treatment for improving bone mass in patients with osteoporosis; however, its effects on bone regeneration are still unclear. The objective of this study was to evaluate the potential toxicity systemic rhPTH, as well as its ability to regenerate critical-sized defects (CZD) in bone. We used 43 female Wistar rats (body weight, 150 ± 50 g). Critical-sized bone defects in rat calvaria received vehicle alone (Control Group, CG) or daily rhPTH (20 ìg/Kg/day) by subcutaneous injection (Experimental Group, EG). We evaluated bone healing obtained at the 1st, 3rd, and 6th wks post-surgery by biochemical, soft x-ray, histological, and morphometric studies. In the EG, at the 1st and 3rd wks, many areas of focal osteoblast hyperplasia were found on parietal bone. At the 3rd wk, woven and/or lamellar bone, in an organized interconnected trabecular network, showed disrupted mineralization. At the 6th wk, looped bone was found to have formed patterns on parietal bone. New bone formed in the EG showed significant statistical differences (p = 0.023) at the 6th wk. Systemic rhPTH at the dose of 20 ìg/Kg/day was able to stimulate bone formation on rat CZD. Also, pre-existing and new bone showed non-proliferative forms of bone hyperostosis (increased non-neoplastic bone).
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Takagi, Shin. Okayama University; Japón
Fil: Nagatsuka, Hotoshi. Okayama University; Japón
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
description Intermittent Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective treatment for improving bone mass in patients with osteoporosis; however, its effects on bone regeneration are still unclear. The objective of this study was to evaluate the potential toxicity systemic rhPTH, as well as its ability to regenerate critical-sized defects (CZD) in bone. We used 43 female Wistar rats (body weight, 150 ± 50 g). Critical-sized bone defects in rat calvaria received vehicle alone (Control Group, CG) or daily rhPTH (20 ìg/Kg/day) by subcutaneous injection (Experimental Group, EG). We evaluated bone healing obtained at the 1st, 3rd, and 6th wks post-surgery by biochemical, soft x-ray, histological, and morphometric studies. In the EG, at the 1st and 3rd wks, many areas of focal osteoblast hyperplasia were found on parietal bone. At the 3rd wk, woven and/or lamellar bone, in an organized interconnected trabecular network, showed disrupted mineralization. At the 6th wk, looped bone was found to have formed patterns on parietal bone. New bone formed in the EG showed significant statistical differences (p = 0.023) at the 6th wk. Systemic rhPTH at the dose of 20 ìg/Kg/day was able to stimulate bone formation on rat CZD. Also, pre-existing and new bone showed non-proliferative forms of bone hyperostosis (increased non-neoplastic bone).
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243519
Jammal, María Victoria; Pastorino, Nina Fiorella; Abate, Carlos Mauricio; Takagi, Shin; Nagatsuka, Hotoshi; et al.; Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH; The Society for Hard Tissue Regenerative Biology; Journal of Hard Tissue Biology; 21; 4; 7-2012; 443-450
1341-7649
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243519
identifier_str_mv Jammal, María Victoria; Pastorino, Nina Fiorella; Abate, Carlos Mauricio; Takagi, Shin; Nagatsuka, Hotoshi; et al.; Bone-Healing Pattern on Critical-Sized Defects Treated by rhPTH; The Society for Hard Tissue Regenerative Biology; Journal of Hard Tissue Biology; 21; 4; 7-2012; 443-450
1341-7649
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/jhtb/21/4/21_443/_article
info:eu-repo/semantics/altIdentifier/doi/10.2485/jhtb.21.443
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv The Society for Hard Tissue Regenerative Biology
publisher.none.fl_str_mv The Society for Hard Tissue Regenerative Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083281630527488
score 13.22299

Publicaciones similares