Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
- Autores
- Jammal, María Victoria; Abate, Carlos Mauricio; Missana, Liliana Raquel
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions.
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica
San Miguel de Tucumán
Argentina
Sociedad Argentina de Investigación Odontológica - Materia
-
critical sized defects
bone regeneration
rhPTH,
hyperostosis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/191403
Ver los metadatos del registro completo
id |
CONICETDig_3c7f3c3c43dcbcae9457d71308a331d9 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/191403 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitenteJammal, María VictoriaAbate, Carlos MauricioMissana, Liliana Raquelcritical sized defectsbone regenerationrhPTH,hyperostosishttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions.Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLIV Reunión Anual Sociedad Argentina de Investigación OdontológicaSan Miguel de TucumánArgentinaSociedad Argentina de Investigación OdontológicaSociedad Argentina de Investigación Odontológica2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.rarapplication/pdfhttp://hdl.handle.net/11336/191403Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente; XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica; San Miguel de Tucumán; Argentina; 2011; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://saio.org.ar/oldsaio/new/descargas/Libro_XLIV_Reunion_Cientifica_Anual.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:44Zoai:ri.conicet.gov.ar:11336/191403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:45.074CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente |
title |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente |
spellingShingle |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente Jammal, María Victoria critical sized defects bone regeneration rhPTH, hyperostosis |
title_short |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente |
title_full |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente |
title_fullStr |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente |
title_full_unstemmed |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente |
title_sort |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente |
dc.creator.none.fl_str_mv |
Jammal, María Victoria Abate, Carlos Mauricio Missana, Liliana Raquel |
author |
Jammal, María Victoria |
author_facet |
Jammal, María Victoria Abate, Carlos Mauricio Missana, Liliana Raquel |
author_role |
author |
author2 |
Abate, Carlos Mauricio Missana, Liliana Raquel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
critical sized defects bone regeneration rhPTH, hyperostosis |
topic |
critical sized defects bone regeneration rhPTH, hyperostosis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions. Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica San Miguel de Tucumán Argentina Sociedad Argentina de Investigación Odontológica |
description |
Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/191403 Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente; XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica; San Miguel de Tucumán; Argentina; 2011; 1-2 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/191403 |
identifier_str_mv |
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente; XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica; San Miguel de Tucumán; Argentina; 2011; 1-2 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://saio.org.ar/oldsaio/new/descargas/Libro_XLIV_Reunion_Cientifica_Anual.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/vnd.rar application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Sociedad Argentina de Investigación Odontológica |
publisher.none.fl_str_mv |
Sociedad Argentina de Investigación Odontológica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846083513148768256 |
score |
13.22299 |