Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente

Autores
Jammal, María Victoria; Abate, Carlos Mauricio; Missana, Liliana Raquel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions.
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica
San Miguel de Tucumán
Argentina
Sociedad Argentina de Investigación Odontológica
Materia
critical sized defects
bone regeneration
rhPTH,
hyperostosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/191403

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oai_identifier_str oai:ri.conicet.gov.ar:11336/191403
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitenteJammal, María VictoriaAbate, Carlos MauricioMissana, Liliana Raquelcritical sized defectsbone regenerationrhPTH,hyperostosishttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions.Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLIV Reunión Anual Sociedad Argentina de Investigación OdontológicaSan Miguel de TucumánArgentinaSociedad Argentina de Investigación OdontológicaSociedad Argentina de Investigación Odontológica2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.rarapplication/pdfhttp://hdl.handle.net/11336/191403Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente; XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica; San Miguel de Tucumán; Argentina; 2011; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://saio.org.ar/oldsaio/new/descargas/Libro_XLIV_Reunion_Cientifica_Anual.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:44Zoai:ri.conicet.gov.ar:11336/191403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:45.074CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
title Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
spellingShingle Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
Jammal, María Victoria
critical sized defects
bone regeneration
rhPTH,
hyperostosis
title_short Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
title_full Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
title_fullStr Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
title_full_unstemmed Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
title_sort Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente
dc.creator.none.fl_str_mv Jammal, María Victoria
Abate, Carlos Mauricio
Missana, Liliana Raquel
author Jammal, María Victoria
author_facet Jammal, María Victoria
Abate, Carlos Mauricio
Missana, Liliana Raquel
author_role author
author2 Abate, Carlos Mauricio
Missana, Liliana Raquel
author2_role author
author
dc.subject.none.fl_str_mv critical sized defects
bone regeneration
rhPTH,
hyperostosis
topic critical sized defects
bone regeneration
rhPTH,
hyperostosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions.
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica
San Miguel de Tucumán
Argentina
Sociedad Argentina de Investigación Odontológica
description Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective osteoporosis treatment. However, its effects on critical bone defects (CBD) are still unclear. The aim of this study was to assess morphological and tissues changes by systemic intermittent rhPTH administration for treatment of CBD. Forty three wistar female rat (body weight 150 ± 50 g) received CBD (5mm) on calvariae. The animals divided in 2 groups: A) Control Group (CG) received vehicle injection and B) Experimental Group (EG): received daily, rhPTH (20ug/kg/day) subcutaneous injection. Samples obtained from animals sacrificed at 1°, 3° and 6° weeks post surgery, were submitted for histological and histometrical studies. The bone formation on edges and CBD area did not regenerate defects. At 1° week, new bone showed basophilic cementales lines, arranged in a swirling pattern, resembling a disrupted mineralization. A focal osteoblasts hyperplasia was observed. At 3° and 6° weeks, there was a significant increase in bone thickness, many sutures were closed and endostic hyperostosis was observed. Large osteocytes showed granular and eosinophilic cytoplasm. Many showed bodies formation like ? Mallory-Denk?. New bone formed in EG exhibited at 1 week (0,84 % ± 0,6) and 3 weeks (0,42 % ± 0,1) compared with CG (0,21 % ± 0,07) and (0,65 % ± 0,2) respectively showing non significant statistical differences. At 6 weeks, EG shows significant bone formed (2, 81 % ± 0,6) compared with CG (1,06 % ± 0,2) (p= 0.023, Mann Whitney Test). Conclusion: rhPTH on calvariae CBD, increase Bone Multicellular Unit activities showing new bone formation on preexisting bone. Also, rhPTH 20ug induce non-neoplastic proliferative lesions.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/191403
Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente; XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica; San Miguel de Tucumán; Argentina; 2011; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/191403
identifier_str_mv Hiperostosis ósea en defectos óseos críticos tratados con rhpth intermitente; XLIV Reunión Anual Sociedad Argentina de Investigación Odontológica; San Miguel de Tucumán; Argentina; 2011; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://saio.org.ar/oldsaio/new/descargas/Libro_XLIV_Reunion_Cientifica_Anual.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.rar
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación Odontológica
publisher.none.fl_str_mv Sociedad Argentina de Investigación Odontológica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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