A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering

Autores
Alvarez Echazú, María Inés; Renou, Sandra Judith; Alvarez, Gisela Solange; Desimone, Martín Federico; Olmedo, Daniel Gustavo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bone is a hierarchical material that has inspired the design of biopolymer-derived biocomposites for tissue engineering purposes. The present study sought to synthesize and perform the physicochemical characterization and biocompatibility of a collagen-silica-based biocomposite for potential application in bone tissue engineering. Ultrastructure, biodegradability, swelling behavior, and biocompatibility properties were analyzed to gain insight into the advantages and limitations to the use of this biomaterial as a bone substitute. Scanning electron microscopy analysis showed a packed-collagen fibril matrix and silica particles in the biocomposite three-dimensional structure. As shown by analysis of in vitro swelling behavior and biodegradability, it would seem that the material swelled soon after implantation and then suffered degradation. Biocompatibility properties were analyzed in vivo 14-days postimplantation using an experimental model in Wistar rats. The biocomposite was placed inside the hematopoietic bone marrow compartment of both tibiae (n = 16). Newly formed woven bone was observed in response to both materials. Unlike the pure-collagen-tissue interface, extensive areas of osseointegration were observed at the biocomposite-tissue interface, which would indicate that silica particles stimulated new bone formation. Agglomerates of finely particulate material with no inflammatory infiltrate or multinucleated giant cells were observed in the bone marrow implanted with the biocomposite. The biocomposite showed good biocompatibility properties. Further studies are necessary to evaluate their biological behavior over time.
Fil: Alvarez Echazú, María Inés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Renou, Sandra Judith. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
Fil: Alvarez, Gisela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Desimone, Martín Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
Materia
BIOCOMPATIBILITY
BIOCOMPOSITE
BONE TISSUE ENGINEERING
COLLAGEN
SILICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148349
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/148349
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A collagen‐silica‐ based biocomposite for potential application in bone tissue engineeringAlvarez Echazú, María InésRenou, Sandra JudithAlvarez, Gisela SolangeDesimone, Martín FedericoOlmedo, Daniel GustavoBIOCOMPATIBILITYBIOCOMPOSITEBONE TISSUE ENGINEERINGCOLLAGENSILICAhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Bone is a hierarchical material that has inspired the design of biopolymer-derived biocomposites for tissue engineering purposes. The present study sought to synthesize and perform the physicochemical characterization and biocompatibility of a collagen-silica-based biocomposite for potential application in bone tissue engineering. Ultrastructure, biodegradability, swelling behavior, and biocompatibility properties were analyzed to gain insight into the advantages and limitations to the use of this biomaterial as a bone substitute. Scanning electron microscopy analysis showed a packed-collagen fibril matrix and silica particles in the biocomposite three-dimensional structure. As shown by analysis of in vitro swelling behavior and biodegradability, it would seem that the material swelled soon after implantation and then suffered degradation. Biocompatibility properties were analyzed in vivo 14-days postimplantation using an experimental model in Wistar rats. The biocomposite was placed inside the hematopoietic bone marrow compartment of both tibiae (n = 16). Newly formed woven bone was observed in response to both materials. Unlike the pure-collagen-tissue interface, extensive areas of osseointegration were observed at the biocomposite-tissue interface, which would indicate that silica particles stimulated new bone formation. Agglomerates of finely particulate material with no inflammatory infiltrate or multinucleated giant cells were observed in the bone marrow implanted with the biocomposite. The biocomposite showed good biocompatibility properties. Further studies are necessary to evaluate their biological behavior over time.Fil: Alvarez Echazú, María Inés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Renou, Sandra Judith. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; ArgentinaFil: Alvarez, Gisela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Desimone, Martín Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; ArgentinaWiley-liss, div John Wiley & Sons Inc.2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148349Alvarez Echazú, María Inés; Renou, Sandra Judith; Alvarez, Gisela Solange; Desimone, Martín Federico; Olmedo, Daniel Gustavo; A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 8-2021; 1-101549-3296CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37291info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.37291info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:31Zoai:ri.conicet.gov.ar:11336/148349instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:31.852CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
title A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
spellingShingle A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
Alvarez Echazú, María Inés
BIOCOMPATIBILITY
BIOCOMPOSITE
BONE TISSUE ENGINEERING
COLLAGEN
SILICA
title_short A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
title_full A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
title_fullStr A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
title_full_unstemmed A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
title_sort A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering
dc.creator.none.fl_str_mv Alvarez Echazú, María Inés
Renou, Sandra Judith
Alvarez, Gisela Solange
Desimone, Martín Federico
Olmedo, Daniel Gustavo
author Alvarez Echazú, María Inés
author_facet Alvarez Echazú, María Inés
Renou, Sandra Judith
Alvarez, Gisela Solange
Desimone, Martín Federico
Olmedo, Daniel Gustavo
author_role author
author2 Renou, Sandra Judith
Alvarez, Gisela Solange
Desimone, Martín Federico
Olmedo, Daniel Gustavo
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOCOMPATIBILITY
BIOCOMPOSITE
BONE TISSUE ENGINEERING
COLLAGEN
SILICA
topic BIOCOMPATIBILITY
BIOCOMPOSITE
BONE TISSUE ENGINEERING
COLLAGEN
SILICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bone is a hierarchical material that has inspired the design of biopolymer-derived biocomposites for tissue engineering purposes. The present study sought to synthesize and perform the physicochemical characterization and biocompatibility of a collagen-silica-based biocomposite for potential application in bone tissue engineering. Ultrastructure, biodegradability, swelling behavior, and biocompatibility properties were analyzed to gain insight into the advantages and limitations to the use of this biomaterial as a bone substitute. Scanning electron microscopy analysis showed a packed-collagen fibril matrix and silica particles in the biocomposite three-dimensional structure. As shown by analysis of in vitro swelling behavior and biodegradability, it would seem that the material swelled soon after implantation and then suffered degradation. Biocompatibility properties were analyzed in vivo 14-days postimplantation using an experimental model in Wistar rats. The biocomposite was placed inside the hematopoietic bone marrow compartment of both tibiae (n = 16). Newly formed woven bone was observed in response to both materials. Unlike the pure-collagen-tissue interface, extensive areas of osseointegration were observed at the biocomposite-tissue interface, which would indicate that silica particles stimulated new bone formation. Agglomerates of finely particulate material with no inflammatory infiltrate or multinucleated giant cells were observed in the bone marrow implanted with the biocomposite. The biocomposite showed good biocompatibility properties. Further studies are necessary to evaluate their biological behavior over time.
Fil: Alvarez Echazú, María Inés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Renou, Sandra Judith. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
Fil: Alvarez, Gisela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Desimone, Martín Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
description Bone is a hierarchical material that has inspired the design of biopolymer-derived biocomposites for tissue engineering purposes. The present study sought to synthesize and perform the physicochemical characterization and biocompatibility of a collagen-silica-based biocomposite for potential application in bone tissue engineering. Ultrastructure, biodegradability, swelling behavior, and biocompatibility properties were analyzed to gain insight into the advantages and limitations to the use of this biomaterial as a bone substitute. Scanning electron microscopy analysis showed a packed-collagen fibril matrix and silica particles in the biocomposite three-dimensional structure. As shown by analysis of in vitro swelling behavior and biodegradability, it would seem that the material swelled soon after implantation and then suffered degradation. Biocompatibility properties were analyzed in vivo 14-days postimplantation using an experimental model in Wistar rats. The biocomposite was placed inside the hematopoietic bone marrow compartment of both tibiae (n = 16). Newly formed woven bone was observed in response to both materials. Unlike the pure-collagen-tissue interface, extensive areas of osseointegration were observed at the biocomposite-tissue interface, which would indicate that silica particles stimulated new bone formation. Agglomerates of finely particulate material with no inflammatory infiltrate or multinucleated giant cells were observed in the bone marrow implanted with the biocomposite. The biocomposite showed good biocompatibility properties. Further studies are necessary to evaluate their biological behavior over time.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148349
Alvarez Echazú, María Inés; Renou, Sandra Judith; Alvarez, Gisela Solange; Desimone, Martín Federico; Olmedo, Daniel Gustavo; A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 8-2021; 1-10
1549-3296
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148349
identifier_str_mv Alvarez Echazú, María Inés; Renou, Sandra Judith; Alvarez, Gisela Solange; Desimone, Martín Federico; Olmedo, Daniel Gustavo; A collagen‐silica‐ based biocomposite for potential application in bone tissue engineering; Wiley-liss, div John Wiley & Sons Inc.; Journal of Biomedical Materials Research Part A; 8-2021; 1-10
1549-3296
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37291
info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.37291
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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