Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers

Autores: Pérez Guijarro, Eva; Karras, Panagiotis; Cifdaloz, Metehan; Martínez Herranz, Raúl; Cañon, Estela; Graña, Osvaldo; Horcajada Reales, Celia; Alonso Curbelo, Direna; Calvo, Tonantzin G.; Gómez-López, Gonzalo; Bellora, Nicolás; Riveiro-Falkenbach, Erica; Ortiz-Romero, Pablo L.; Rodríguez-Peralto, José L.; Maestre, Lorena; Roncador, Giovanna; De Agustín Asensio, Juan C.; Goding, Colin R.; Eyras, Eduardo; Megiás, Diego; Méndez, Raúl; Soengas, Maria S.
Año de publicación: 2016
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Nuclear 3'-end-polyadenylation is essential for the transport, stability and translation of virtually all eukaryotic mRNAs. Poly(A) tail extension can also occur in the cytoplasm, but the transcripts involved are incompletely understood, particularly in cancer. Here we identify a lineage-specific requirement of the cytoplasmic polyadenylation binding protein 4 (CPEB4) in malignant melanoma. CPEB4 is upregulated early in melanoma progression, as defined by computational and histological analyses. Melanoma cells are distinct from other tumour cell types in their dependency on CPEB4, not only to prevent mitotic aberrations, but to progress through G1/S cell cycle checkpoints. RNA immunoprecipitation, sequencing of bound transcripts and poly(A) length tests link the melanoma-specific functions of CPEB4 to signalling hubs specifically enriched in this disease. Essential in these CPEB4-controlled networks are the melanoma drivers MITF and RAB7A, a feature validated in clinical biopsies. These results provide new mechanistic links between cytoplasmic polyadenylation and lineage specification in melanoma.
Fil: Pérez Guijarro, Eva. Centro Nacional de Investigaciones Oncologicas; España
Fil: Karras, Panagiotis. Centro Nacional de Investigaciones Oncologicas; España
Fil: Cifdaloz, Metehan. Centro Nacional de Investigaciones Oncologicas; España
Fil: Martínez Herranz, Raúl. Centro Nacional de Investigaciones Oncologicas; España
Fil: Cañon, Estela. Centro Nacional de Investigaciones Oncologicas; España
Fil: Graña, Osvaldo. Centro Nacional de Investigaciones Oncologicas; España
Fil: Horcajada Reales, Celia. Hospital General Universitario ; España
Fil: Alonso Curbelo, Direna. Memorial Sloan Kettering Cancer Center; Estados Unidos. Centro Nacional de Investigaciones Oncologicas; España
Fil: Calvo, Tonantzin G.. Centro Nacional de Investigaciones Oncologicas; España
Fil: Gómez-López, Gonzalo. Centro Nacional de Investigaciones Oncologicas; España
Fil: Bellora, Nicolás. Universitat Pompeu Fabra; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Riveiro-Falkenbach, Erica. Universidad Complutense de Madrid; España
Fil: Ortiz-Romero, Pablo L.. Universidad Complutense de Madrid; España
Fil: Rodríguez-Peralto, José L.. Universidad Complutense de Madrid; España
Fil: Maestre, Lorena. Centro Nacional de Investigaciones Oncologicas; España
Fil: Roncador, Giovanna. Centro Nacional de Investigaciones Oncologicas; España
Fil: De Agustín Asensio, Juan C.. Hospital General Universitario ; España
Fil: Goding, Colin R.. Nuffield Department Of Clinical Medicine; Reino Unido
Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; España
Fil: Megiás, Diego. Centro Nacional de Investigaciones Oncologicas; España
Fil: Méndez, Raúl. Institució Catalana de Recerca i Estudis Avancats; España
Fil: Soengas, Maria S.. Centro Nacional de Investigaciones Oncologicas; España
Materia: Melanoma
polyadenylation
lineage specification
CPEB4
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/62962

Acceder

id	CONICETDig_9ce83d9845a377948e2940137d6f2314
oai_identifier_str	oai:ri.conicet.gov.ar:11336/62962
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma driversPérez Guijarro, EvaKarras, PanagiotisCifdaloz, MetehanMartínez Herranz, RaúlCañon, EstelaGraña, OsvaldoHorcajada Reales, CeliaAlonso Curbelo, DirenaCalvo, Tonantzin G.Gómez-López, GonzaloBellora, NicolásRiveiro-Falkenbach, EricaOrtiz-Romero, Pablo L.Rodríguez-Peralto, José L.Maestre, LorenaRoncador, GiovannaDe Agustín Asensio, Juan C.Goding, Colin R.Eyras, EduardoMegiás, DiegoMéndez, RaúlSoengas, Maria S.Melanomapolyadenylationlineage specificationCPEB4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nuclear 3'-end-polyadenylation is essential for the transport, stability and translation of virtually all eukaryotic mRNAs. Poly(A) tail extension can also occur in the cytoplasm, but the transcripts involved are incompletely understood, particularly in cancer. Here we identify a lineage-specific requirement of the cytoplasmic polyadenylation binding protein 4 (CPEB4) in malignant melanoma. CPEB4 is upregulated early in melanoma progression, as defined by computational and histological analyses. Melanoma cells are distinct from other tumour cell types in their dependency on CPEB4, not only to prevent mitotic aberrations, but to progress through G1/S cell cycle checkpoints. RNA immunoprecipitation, sequencing of bound transcripts and poly(A) length tests link the melanoma-specific functions of CPEB4 to signalling hubs specifically enriched in this disease. Essential in these CPEB4-controlled networks are the melanoma drivers MITF and RAB7A, a feature validated in clinical biopsies. These results provide new mechanistic links between cytoplasmic polyadenylation and lineage specification in melanoma.Fil: Pérez Guijarro, Eva. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Karras, Panagiotis. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Cifdaloz, Metehan. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Martínez Herranz, Raúl. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Cañon, Estela. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Graña, Osvaldo. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Horcajada Reales, Celia. Hospital General Universitario ; EspañaFil: Alonso Curbelo, Direna. Memorial Sloan Kettering Cancer Center; Estados Unidos. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Calvo, Tonantzin G.. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Gómez-López, Gonzalo. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Bellora, Nicolás. Universitat Pompeu Fabra; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Riveiro-Falkenbach, Erica. Universidad Complutense de Madrid; EspañaFil: Ortiz-Romero, Pablo L.. Universidad Complutense de Madrid; EspañaFil: Rodríguez-Peralto, José L.. Universidad Complutense de Madrid; EspañaFil: Maestre, Lorena. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Roncador, Giovanna. Centro Nacional de Investigaciones Oncologicas; EspañaFil: De Agustín Asensio, Juan C.. Hospital General Universitario ; EspañaFil: Goding, Colin R.. Nuffield Department Of Clinical Medicine; Reino UnidoFil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; EspañaFil: Megiás, Diego. Centro Nacional de Investigaciones Oncologicas; EspañaFil: Méndez, Raúl. Institució Catalana de Recerca i Estudis Avancats; EspañaFil: Soengas, Maria S.. Centro Nacional de Investigaciones Oncologicas; EspañaNature Publishing Group2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62962Pérez Guijarro, Eva; Karras, Panagiotis; Cifdaloz, Metehan; Martínez Herranz, Raúl; Cañon, Estela; et al.; Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers; Nature Publishing Group; Nature Communications; 7; 11-2016; 1-172041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms13418info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms13418info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:49Zoai:ri.conicet.gov.ar:11336/62962instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:49.432CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers
title	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers
spellingShingle	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers Pérez Guijarro, Eva Melanoma polyadenylation lineage specification CPEB4
title_short	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers
title_full	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers
title_fullStr	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers
title_full_unstemmed	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers
title_sort	Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers
dc.creator.none.fl_str_mv	Pérez Guijarro, Eva Karras, Panagiotis Cifdaloz, Metehan Martínez Herranz, Raúl Cañon, Estela Graña, Osvaldo Horcajada Reales, Celia Alonso Curbelo, Direna Calvo, Tonantzin G. Gómez-López, Gonzalo Bellora, Nicolás Riveiro-Falkenbach, Erica Ortiz-Romero, Pablo L. Rodríguez-Peralto, José L. Maestre, Lorena Roncador, Giovanna De Agustín Asensio, Juan C. Goding, Colin R. Eyras, Eduardo Megiás, Diego Méndez, Raúl Soengas, Maria S.
author	Pérez Guijarro, Eva
author_facet	Pérez Guijarro, Eva Karras, Panagiotis Cifdaloz, Metehan Martínez Herranz, Raúl Cañon, Estela Graña, Osvaldo Horcajada Reales, Celia Alonso Curbelo, Direna Calvo, Tonantzin G. Gómez-López, Gonzalo Bellora, Nicolás Riveiro-Falkenbach, Erica Ortiz-Romero, Pablo L. Rodríguez-Peralto, José L. Maestre, Lorena Roncador, Giovanna De Agustín Asensio, Juan C. Goding, Colin R. Eyras, Eduardo Megiás, Diego Méndez, Raúl Soengas, Maria S.
author_role	author
author2	Karras, Panagiotis Cifdaloz, Metehan Martínez Herranz, Raúl Cañon, Estela Graña, Osvaldo Horcajada Reales, Celia Alonso Curbelo, Direna Calvo, Tonantzin G. Gómez-López, Gonzalo Bellora, Nicolás Riveiro-Falkenbach, Erica Ortiz-Romero, Pablo L. Rodríguez-Peralto, José L. Maestre, Lorena Roncador, Giovanna De Agustín Asensio, Juan C. Goding, Colin R. Eyras, Eduardo Megiás, Diego Méndez, Raúl Soengas, Maria S.
author2_role	author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Melanoma polyadenylation lineage specification CPEB4
topic	Melanoma polyadenylation lineage specification CPEB4
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Nuclear 3'-end-polyadenylation is essential for the transport, stability and translation of virtually all eukaryotic mRNAs. Poly(A) tail extension can also occur in the cytoplasm, but the transcripts involved are incompletely understood, particularly in cancer. Here we identify a lineage-specific requirement of the cytoplasmic polyadenylation binding protein 4 (CPEB4) in malignant melanoma. CPEB4 is upregulated early in melanoma progression, as defined by computational and histological analyses. Melanoma cells are distinct from other tumour cell types in their dependency on CPEB4, not only to prevent mitotic aberrations, but to progress through G1/S cell cycle checkpoints. RNA immunoprecipitation, sequencing of bound transcripts and poly(A) length tests link the melanoma-specific functions of CPEB4 to signalling hubs specifically enriched in this disease. Essential in these CPEB4-controlled networks are the melanoma drivers MITF and RAB7A, a feature validated in clinical biopsies. These results provide new mechanistic links between cytoplasmic polyadenylation and lineage specification in melanoma. Fil: Pérez Guijarro, Eva. Centro Nacional de Investigaciones Oncologicas; España Fil: Karras, Panagiotis. Centro Nacional de Investigaciones Oncologicas; España Fil: Cifdaloz, Metehan. Centro Nacional de Investigaciones Oncologicas; España Fil: Martínez Herranz, Raúl. Centro Nacional de Investigaciones Oncologicas; España Fil: Cañon, Estela. Centro Nacional de Investigaciones Oncologicas; España Fil: Graña, Osvaldo. Centro Nacional de Investigaciones Oncologicas; España Fil: Horcajada Reales, Celia. Hospital General Universitario ; España Fil: Alonso Curbelo, Direna. Memorial Sloan Kettering Cancer Center; Estados Unidos. Centro Nacional de Investigaciones Oncologicas; España Fil: Calvo, Tonantzin G.. Centro Nacional de Investigaciones Oncologicas; España Fil: Gómez-López, Gonzalo. Centro Nacional de Investigaciones Oncologicas; España Fil: Bellora, Nicolás. Universitat Pompeu Fabra; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Riveiro-Falkenbach, Erica. Universidad Complutense de Madrid; España Fil: Ortiz-Romero, Pablo L.. Universidad Complutense de Madrid; España Fil: Rodríguez-Peralto, José L.. Universidad Complutense de Madrid; España Fil: Maestre, Lorena. Centro Nacional de Investigaciones Oncologicas; España Fil: Roncador, Giovanna. Centro Nacional de Investigaciones Oncologicas; España Fil: De Agustín Asensio, Juan C.. Hospital General Universitario ; España Fil: Goding, Colin R.. Nuffield Department Of Clinical Medicine; Reino Unido Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; España Fil: Megiás, Diego. Centro Nacional de Investigaciones Oncologicas; España Fil: Méndez, Raúl. Institució Catalana de Recerca i Estudis Avancats; España Fil: Soengas, Maria S.. Centro Nacional de Investigaciones Oncologicas; España
description	Nuclear 3'-end-polyadenylation is essential for the transport, stability and translation of virtually all eukaryotic mRNAs. Poly(A) tail extension can also occur in the cytoplasm, but the transcripts involved are incompletely understood, particularly in cancer. Here we identify a lineage-specific requirement of the cytoplasmic polyadenylation binding protein 4 (CPEB4) in malignant melanoma. CPEB4 is upregulated early in melanoma progression, as defined by computational and histological analyses. Melanoma cells are distinct from other tumour cell types in their dependency on CPEB4, not only to prevent mitotic aberrations, but to progress through G1/S cell cycle checkpoints. RNA immunoprecipitation, sequencing of bound transcripts and poly(A) length tests link the melanoma-specific functions of CPEB4 to signalling hubs specifically enriched in this disease. Essential in these CPEB4-controlled networks are the melanoma drivers MITF and RAB7A, a feature validated in clinical biopsies. These results provide new mechanistic links between cytoplasmic polyadenylation and lineage specification in melanoma.
publishDate	2016
dc.date.none.fl_str_mv	2016-11
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/62962 Pérez Guijarro, Eva; Karras, Panagiotis; Cifdaloz, Metehan; Martínez Herranz, Raúl; Cañon, Estela; et al.; Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers; Nature Publishing Group; Nature Communications; 7; 11-2016; 1-17 2041-1723 CONICET Digital CONICET
url	http://hdl.handle.net/11336/62962
identifier_str_mv	Pérez Guijarro, Eva; Karras, Panagiotis; Cifdaloz, Metehan; Martínez Herranz, Raúl; Cañon, Estela; et al.; Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers; Nature Publishing Group; Nature Communications; 7; 11-2016; 1-17 2041-1723 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms13418 info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms13418
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Nature Publishing Group
publisher.none.fl_str_mv	Nature Publishing Group
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1844614412168593408
score	13.070432

Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers

Publicaciones similares