Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3

Autores
Savtchouk, Iaroslav; Sun, Lu; Bender, Crhistian Luis; Lee, Hyang Burm; Szabó, Gábor; Gasparini, Sonia; Liu, Siqiong June
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Synaptic receptors gate the neuronal response to incoming signals, but they are not homogeneously distributed on dendrites. A spatially defined receptor distribution can preferentially amplify certain synaptic inputs, resize receptive fields of neurons, and optimize information processing within a neuronal circuit. Thus, a longstanding question is how the spatial organization of synaptic receptors is achieved. Here, we find that action potentials provide local signals that influence the distribution of synaptic AMPA receptors along dendrites in mouse cerebellar stellate cells. Graded dendritic depolarizations elevate CPEB3 protein at proximal dendrites, where we suggest that CPEB3 binds to GluA2 mRNA, suppressing GluA2 protein synthesis leading to a distance-dependent increase in synaptic GluA2 AMPARs. The activity-induced expression of CPEB3 requires increased Ca2+ and PKC activation. Our results suggest a cell-autonomous mechanism where sustained postsynaptic firing drives graded local protein synthesis, thus directing the spatial organization of synaptic AMPARs.
Fil: Savtchouk, Iaroslav. University of Pennsylvania; Estados Unidos. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos
Fil: Sun, Lu. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados Unidos
Fil: Bender, Crhistian Luis. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Lee, Hyang Burm. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados Unidos
Fil: Szabó, Gábor. Institute Of Experimental Medicine Hungarian Academy Of Sciences; . LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. Institute of Experimental Medicine. Laboratory of Molecular Biology and Genetics; Hungría
Fil: Gasparini, Sonia. LSU Health Sciences Center. Neuroscience Center of Excellence; Estados Unidos. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos
Fil: Liu, Siqiong June. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados Unidos
Materia
ACTION POTENTIALS
AMPA RECEPTOR
CALCIUM IMAGING
CEREBELLAR STELLATE CELL
CPEB3
DENDRITIC GRADIENT
GLUA2
PKC
SUBUNIT COMPOSITION
TOPOSTATIC PLASTICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50350
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50350
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3Savtchouk, IaroslavSun, LuBender, Crhistian LuisLee, Hyang BurmSzabó, GáborGasparini, SoniaLiu, Siqiong JuneACTION POTENTIALSAMPA RECEPTORCALCIUM IMAGINGCEREBELLAR STELLATE CELLCPEB3DENDRITIC GRADIENTGLUA2PKCSUBUNIT COMPOSITIONTOPOSTATIC PLASTICITYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Synaptic receptors gate the neuronal response to incoming signals, but they are not homogeneously distributed on dendrites. A spatially defined receptor distribution can preferentially amplify certain synaptic inputs, resize receptive fields of neurons, and optimize information processing within a neuronal circuit. Thus, a longstanding question is how the spatial organization of synaptic receptors is achieved. Here, we find that action potentials provide local signals that influence the distribution of synaptic AMPA receptors along dendrites in mouse cerebellar stellate cells. Graded dendritic depolarizations elevate CPEB3 protein at proximal dendrites, where we suggest that CPEB3 binds to GluA2 mRNA, suppressing GluA2 protein synthesis leading to a distance-dependent increase in synaptic GluA2 AMPARs. The activity-induced expression of CPEB3 requires increased Ca2+ and PKC activation. Our results suggest a cell-autonomous mechanism where sustained postsynaptic firing drives graded local protein synthesis, thus directing the spatial organization of synaptic AMPARs.Fil: Savtchouk, Iaroslav. University of Pennsylvania; Estados Unidos. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados UnidosFil: Sun, Lu. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados UnidosFil: Bender, Crhistian Luis. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Lee, Hyang Burm. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados UnidosFil: Szabó, Gábor. Institute Of Experimental Medicine Hungarian Academy Of Sciences; . LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. Institute of Experimental Medicine. Laboratory of Molecular Biology and Genetics; HungríaFil: Gasparini, Sonia. LSU Health Sciences Center. Neuroscience Center of Excellence; Estados Unidos. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados UnidosFil: Liu, Siqiong June. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados UnidosElsevier B.V.2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50350Savtchouk, Iaroslav; Sun, Lu; Bender, Crhistian Luis; Lee, Hyang Burm; Szabó, Gábor; et al.; Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3; Elsevier B.V.; Cell Reports; 17; 1; 9-2016; 86-1032211-12472211-1247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.08.094info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124716312037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:16Zoai:ri.conicet.gov.ar:11336/50350instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:16.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
title Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
spellingShingle Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
Savtchouk, Iaroslav
ACTION POTENTIALS
AMPA RECEPTOR
CALCIUM IMAGING
CEREBELLAR STELLATE CELL
CPEB3
DENDRITIC GRADIENT
GLUA2
PKC
SUBUNIT COMPOSITION
TOPOSTATIC PLASTICITY
title_short Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
title_full Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
title_fullStr Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
title_full_unstemmed Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
title_sort Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3
dc.creator.none.fl_str_mv Savtchouk, Iaroslav
Sun, Lu
Bender, Crhistian Luis
Lee, Hyang Burm
Szabó, Gábor
Gasparini, Sonia
Liu, Siqiong June
author Savtchouk, Iaroslav
author_facet Savtchouk, Iaroslav
Sun, Lu
Bender, Crhistian Luis
Lee, Hyang Burm
Szabó, Gábor
Gasparini, Sonia
Liu, Siqiong June
author_role author
author2 Sun, Lu
Bender, Crhistian Luis
Lee, Hyang Burm
Szabó, Gábor
Gasparini, Sonia
Liu, Siqiong June
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ACTION POTENTIALS
AMPA RECEPTOR
CALCIUM IMAGING
CEREBELLAR STELLATE CELL
CPEB3
DENDRITIC GRADIENT
GLUA2
PKC
SUBUNIT COMPOSITION
TOPOSTATIC PLASTICITY
topic ACTION POTENTIALS
AMPA RECEPTOR
CALCIUM IMAGING
CEREBELLAR STELLATE CELL
CPEB3
DENDRITIC GRADIENT
GLUA2
PKC
SUBUNIT COMPOSITION
TOPOSTATIC PLASTICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Synaptic receptors gate the neuronal response to incoming signals, but they are not homogeneously distributed on dendrites. A spatially defined receptor distribution can preferentially amplify certain synaptic inputs, resize receptive fields of neurons, and optimize information processing within a neuronal circuit. Thus, a longstanding question is how the spatial organization of synaptic receptors is achieved. Here, we find that action potentials provide local signals that influence the distribution of synaptic AMPA receptors along dendrites in mouse cerebellar stellate cells. Graded dendritic depolarizations elevate CPEB3 protein at proximal dendrites, where we suggest that CPEB3 binds to GluA2 mRNA, suppressing GluA2 protein synthesis leading to a distance-dependent increase in synaptic GluA2 AMPARs. The activity-induced expression of CPEB3 requires increased Ca2+ and PKC activation. Our results suggest a cell-autonomous mechanism where sustained postsynaptic firing drives graded local protein synthesis, thus directing the spatial organization of synaptic AMPARs.
Fil: Savtchouk, Iaroslav. University of Pennsylvania; Estados Unidos. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos
Fil: Sun, Lu. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados Unidos
Fil: Bender, Crhistian Luis. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Lee, Hyang Burm. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados Unidos
Fil: Szabó, Gábor. Institute Of Experimental Medicine Hungarian Academy Of Sciences; . LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. Institute of Experimental Medicine. Laboratory of Molecular Biology and Genetics; Hungría
Fil: Gasparini, Sonia. LSU Health Sciences Center. Neuroscience Center of Excellence; Estados Unidos. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos
Fil: Liu, Siqiong June. LSU Health Sciences Center. Department of Cell Biology and Anatomy; Estados Unidos. University of Pennsylvania; Estados Unidos
description Synaptic receptors gate the neuronal response to incoming signals, but they are not homogeneously distributed on dendrites. A spatially defined receptor distribution can preferentially amplify certain synaptic inputs, resize receptive fields of neurons, and optimize information processing within a neuronal circuit. Thus, a longstanding question is how the spatial organization of synaptic receptors is achieved. Here, we find that action potentials provide local signals that influence the distribution of synaptic AMPA receptors along dendrites in mouse cerebellar stellate cells. Graded dendritic depolarizations elevate CPEB3 protein at proximal dendrites, where we suggest that CPEB3 binds to GluA2 mRNA, suppressing GluA2 protein synthesis leading to a distance-dependent increase in synaptic GluA2 AMPARs. The activity-induced expression of CPEB3 requires increased Ca2+ and PKC activation. Our results suggest a cell-autonomous mechanism where sustained postsynaptic firing drives graded local protein synthesis, thus directing the spatial organization of synaptic AMPARs.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50350
Savtchouk, Iaroslav; Sun, Lu; Bender, Crhistian Luis; Lee, Hyang Burm; Szabó, Gábor; et al.; Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3; Elsevier B.V.; Cell Reports; 17; 1; 9-2016; 86-103
2211-1247
2211-1247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50350
identifier_str_mv Savtchouk, Iaroslav; Sun, Lu; Bender, Crhistian Luis; Lee, Hyang Burm; Szabó, Gábor; et al.; Topological regulation of synaptic AMPA receptor expression by the RNA-Binding protein CPEB3; Elsevier B.V.; Cell Reports; 17; 1; 9-2016; 86-103
2211-1247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.08.094
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124716312037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842979937675378688
score 12.48226

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