Emotional stress induces structural plasticity in Bergmann glial cells via an AC5–CPEB3–GluA1 pathway

• Autores: Bender, Crhistian Luis; Sun, Xingxing; Farooq, Muhammad; Yang, Qian; Davison, Caroline; Maroteaux, Matthieu; Huang, Yi Shuian; Ishikawa, Yoshihiro; Liu, Siqiong June
• Año de publicación: 2020
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: ADRENERGIC RECEPTORS; ASTROCYTIC REMODELING; BERGMANN GLIAL CELLS; CPEB3; GLIAL PROCESSES; GLUA1
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Stress alters brain function by modifying the structure and function of neurons and astrocytes. The fine processes of astrocytes are critical for the clearance of neurotransmitters during synaptic transmission. Thus, experience-dependent remodeling of glial processes is anticipated to alter the output of neural circuits. However, the molecular mechanisms that underlie glial structural plasticity are not known. Here we show that a single exposure of male and female mice to an acute stress produced a long-lasting retraction of the lateral processes of cerebellar Bergmann glial cells. These cells express the GluA1 subunit of AMPA-type glutamate receptors, and GluA1 knockdown is known to shorten the length of glial processes. We found that stress reduced the level of GluA1 protein and AMPA receptor-mediated currents in Bergmann glial cells, and these effects were absent in mice devoid of CPEB3, a protein that binds to GluA1 mRNA and regulates GluA1 protein synthesis. Administration of a b-adrenergic receptor blocker attenuated the reduction in GluA1, and deletion of adenylate cyclase 5 prevented GluA1 suppression. Therefore, stress suppresses GluA1 protein synthesis via an adrenergic/adenylyl cyclase/CPEB3 pathway, and reduces the length of astrocyte lateral processes. Our results identify a novel mechanism for GluA1 subunit plasticity in non-neuronal cells and suggest a previously unappreciated role for AMPA receptors in stress-induced astrocytic remodeling.
Fil: Bender, Crhistian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina. State University of Louisiana; Estados Unidos
Fil: Sun, Xingxing. Huazhong University of Science & Technology; República de China. State University of Louisiana; Estados Unidos
Fil: Farooq, Muhammad. State University of Louisiana; Estados Unidos
Fil: Yang, Qian. State University of Louisiana; Estados Unidos
Fil: Davison, Caroline. State University of Louisiana; Estados Unidos
Fil: Maroteaux, Matthieu. State University of Louisiana; Estados Unidos
Fil: Huang, Yi Shuian. State University of Louisiana; Estados Unidos
Fil: Ishikawa, Yoshihiro. State University of Louisiana; Estados Unidos. Yokohama City University. Graduate School of Medicine; Japón
Fil: Liu, Siqiong June. State University of Louisiana; Estados Unidos