mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4

Autores
Pascual, Rosa; Segura Morales, Carolina; Omerzu, Manja; Bellora, Nicolás; Belloc, Eulàlia; Castellazzi, Chiara Lara; Reina, Oscar; Eyras, Eduardo; Maurice, Madelon M.; Millanes Romero, Alba; Méndez, Raúl
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Transition through cell cycle phases requires temporal and spatial regulation of gene expression to ensure accurate chromosome duplication and segregation. This regulation involves dynamic reprogramming of gene expression at multiple transcriptional and posttranscriptional levels. In transcriptionally silent oocytes, the CPEB-family of RNA-binding proteins coordinates temporal and spatial translation regulation of stored maternal mRNAs to drive meiotic progression. CPEB1 mediates mRNA localization to the meiotic spindle, which is required to ensure proper chromosome segregation. Temporal translational regulation also takes place in mitosis, where a large repertoire of transcripts is activated or repressed in specific cell cycle phases. However, whether control of localized translation at the spindle is required for mitosis is unclear, as mitotic and acentriolar-meiotic spindles are functionally and structurally different. Furthermore, the large differences in scale-ratio between cell volume and spindle size in oocytes compared to somatic mitotic cells may generate distinct requirements for gene expression compartmentalization in meiosis and mitosis. Here we show that mitotic spindles contain CPE-localized mRNAs and translating ribosomes. Moreover, CPEB1 and CPEB4 localize in the spindles and they may function sequentially in promoting mitotic stage transitions and correct chromosome segregation. Thus, CPEB1 and CPEB4 bind to specific spindle-associated transcripts controlling the expression and/or localization of their encoded factors that, respectively, drive metaphase and anaphase/cytokinesis.
Fil: Pascual, Rosa. Barcelona Institute Of Science And Technology.; España
Fil: Segura Morales, Carolina. Barcelona Institute Of Science And Technology.; España
Fil: Omerzu, Manja. University of Utrecht; Países Bajos
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Belloc, Eulàlia. Barcelona Institute Of Science And Technology.; España
Fil: Castellazzi, Chiara Lara. Barcelona Institute Of Science And Technology.; España
Fil: Reina, Oscar. Barcelona Institute Of Science And Technology.; España
Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avançats; España
Fil: Maurice, Madelon M.. University of Utrecht; Países Bajos
Fil: Millanes Romero, Alba. Barcelona Institute Of Science And Technology.; España
Fil: Méndez, Raúl. Barcelona Institute Of Science And Technology.; España
Materia
CPEB
MITOSIS
MRNA LOCALIZATION
MRNA TRANSLATION
SPINDLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174742
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/174742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4Pascual, RosaSegura Morales, CarolinaOmerzu, ManjaBellora, NicolásBelloc, EulàliaCastellazzi, Chiara LaraReina, OscarEyras, EduardoMaurice, Madelon M.Millanes Romero, AlbaMéndez, RaúlCPEBMITOSISMRNA LOCALIZATIONMRNA TRANSLATIONSPINDLEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Transition through cell cycle phases requires temporal and spatial regulation of gene expression to ensure accurate chromosome duplication and segregation. This regulation involves dynamic reprogramming of gene expression at multiple transcriptional and posttranscriptional levels. In transcriptionally silent oocytes, the CPEB-family of RNA-binding proteins coordinates temporal and spatial translation regulation of stored maternal mRNAs to drive meiotic progression. CPEB1 mediates mRNA localization to the meiotic spindle, which is required to ensure proper chromosome segregation. Temporal translational regulation also takes place in mitosis, where a large repertoire of transcripts is activated or repressed in specific cell cycle phases. However, whether control of localized translation at the spindle is required for mitosis is unclear, as mitotic and acentriolar-meiotic spindles are functionally and structurally different. Furthermore, the large differences in scale-ratio between cell volume and spindle size in oocytes compared to somatic mitotic cells may generate distinct requirements for gene expression compartmentalization in meiosis and mitosis. Here we show that mitotic spindles contain CPE-localized mRNAs and translating ribosomes. Moreover, CPEB1 and CPEB4 localize in the spindles and they may function sequentially in promoting mitotic stage transitions and correct chromosome segregation. Thus, CPEB1 and CPEB4 bind to specific spindle-associated transcripts controlling the expression and/or localization of their encoded factors that, respectively, drive metaphase and anaphase/cytokinesis.Fil: Pascual, Rosa. Barcelona Institute Of Science And Technology.; EspañaFil: Segura Morales, Carolina. Barcelona Institute Of Science And Technology.; EspañaFil: Omerzu, Manja. University of Utrecht; Países BajosFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Belloc, Eulàlia. Barcelona Institute Of Science And Technology.; EspañaFil: Castellazzi, Chiara Lara. Barcelona Institute Of Science And Technology.; EspañaFil: Reina, Oscar. Barcelona Institute Of Science And Technology.; EspañaFil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avançats; EspañaFil: Maurice, Madelon M.. University of Utrecht; Países BajosFil: Millanes Romero, Alba. Barcelona Institute Of Science And Technology.; EspañaFil: Méndez, Raúl. Barcelona Institute Of Science And Technology.; EspañaCold Spring Harbor Laboratory Press2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174742Pascual, Rosa; Segura Morales, Carolina; Omerzu, Manja; Bellora, Nicolás; Belloc, Eulàlia; et al.; mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4; Cold Spring Harbor Laboratory Press; RNA (New York, N.Y.); 27; 3; 3-2021; 291-3021355-83821469-9001CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://rnajournal.cshlp.org/lookup/doi/10.1261/rna.077552.120info:eu-repo/semantics/altIdentifier/doi/10.1261/rna.077552.120info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:01Zoai:ri.conicet.gov.ar:11336/174742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:01.485CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
title mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
spellingShingle mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
Pascual, Rosa
CPEB
MITOSIS
MRNA LOCALIZATION
MRNA TRANSLATION
SPINDLE
title_short mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
title_full mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
title_fullStr mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
title_full_unstemmed mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
title_sort mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4
dc.creator.none.fl_str_mv Pascual, Rosa
Segura Morales, Carolina
Omerzu, Manja
Bellora, Nicolás
Belloc, Eulàlia
Castellazzi, Chiara Lara
Reina, Oscar
Eyras, Eduardo
Maurice, Madelon M.
Millanes Romero, Alba
Méndez, Raúl
author Pascual, Rosa
author_facet Pascual, Rosa
Segura Morales, Carolina
Omerzu, Manja
Bellora, Nicolás
Belloc, Eulàlia
Castellazzi, Chiara Lara
Reina, Oscar
Eyras, Eduardo
Maurice, Madelon M.
Millanes Romero, Alba
Méndez, Raúl
author_role author
author2 Segura Morales, Carolina
Omerzu, Manja
Bellora, Nicolás
Belloc, Eulàlia
Castellazzi, Chiara Lara
Reina, Oscar
Eyras, Eduardo
Maurice, Madelon M.
Millanes Romero, Alba
Méndez, Raúl
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CPEB
MITOSIS
MRNA LOCALIZATION
MRNA TRANSLATION
SPINDLE
topic CPEB
MITOSIS
MRNA LOCALIZATION
MRNA TRANSLATION
SPINDLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Transition through cell cycle phases requires temporal and spatial regulation of gene expression to ensure accurate chromosome duplication and segregation. This regulation involves dynamic reprogramming of gene expression at multiple transcriptional and posttranscriptional levels. In transcriptionally silent oocytes, the CPEB-family of RNA-binding proteins coordinates temporal and spatial translation regulation of stored maternal mRNAs to drive meiotic progression. CPEB1 mediates mRNA localization to the meiotic spindle, which is required to ensure proper chromosome segregation. Temporal translational regulation also takes place in mitosis, where a large repertoire of transcripts is activated or repressed in specific cell cycle phases. However, whether control of localized translation at the spindle is required for mitosis is unclear, as mitotic and acentriolar-meiotic spindles are functionally and structurally different. Furthermore, the large differences in scale-ratio between cell volume and spindle size in oocytes compared to somatic mitotic cells may generate distinct requirements for gene expression compartmentalization in meiosis and mitosis. Here we show that mitotic spindles contain CPE-localized mRNAs and translating ribosomes. Moreover, CPEB1 and CPEB4 localize in the spindles and they may function sequentially in promoting mitotic stage transitions and correct chromosome segregation. Thus, CPEB1 and CPEB4 bind to specific spindle-associated transcripts controlling the expression and/or localization of their encoded factors that, respectively, drive metaphase and anaphase/cytokinesis.
Fil: Pascual, Rosa. Barcelona Institute Of Science And Technology.; España
Fil: Segura Morales, Carolina. Barcelona Institute Of Science And Technology.; España
Fil: Omerzu, Manja. University of Utrecht; Países Bajos
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Belloc, Eulàlia. Barcelona Institute Of Science And Technology.; España
Fil: Castellazzi, Chiara Lara. Barcelona Institute Of Science And Technology.; España
Fil: Reina, Oscar. Barcelona Institute Of Science And Technology.; España
Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avançats; España
Fil: Maurice, Madelon M.. University of Utrecht; Países Bajos
Fil: Millanes Romero, Alba. Barcelona Institute Of Science And Technology.; España
Fil: Méndez, Raúl. Barcelona Institute Of Science And Technology.; España
description Transition through cell cycle phases requires temporal and spatial regulation of gene expression to ensure accurate chromosome duplication and segregation. This regulation involves dynamic reprogramming of gene expression at multiple transcriptional and posttranscriptional levels. In transcriptionally silent oocytes, the CPEB-family of RNA-binding proteins coordinates temporal and spatial translation regulation of stored maternal mRNAs to drive meiotic progression. CPEB1 mediates mRNA localization to the meiotic spindle, which is required to ensure proper chromosome segregation. Temporal translational regulation also takes place in mitosis, where a large repertoire of transcripts is activated or repressed in specific cell cycle phases. However, whether control of localized translation at the spindle is required for mitosis is unclear, as mitotic and acentriolar-meiotic spindles are functionally and structurally different. Furthermore, the large differences in scale-ratio between cell volume and spindle size in oocytes compared to somatic mitotic cells may generate distinct requirements for gene expression compartmentalization in meiosis and mitosis. Here we show that mitotic spindles contain CPE-localized mRNAs and translating ribosomes. Moreover, CPEB1 and CPEB4 localize in the spindles and they may function sequentially in promoting mitotic stage transitions and correct chromosome segregation. Thus, CPEB1 and CPEB4 bind to specific spindle-associated transcripts controlling the expression and/or localization of their encoded factors that, respectively, drive metaphase and anaphase/cytokinesis.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174742
Pascual, Rosa; Segura Morales, Carolina; Omerzu, Manja; Bellora, Nicolás; Belloc, Eulàlia; et al.; mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4; Cold Spring Harbor Laboratory Press; RNA (New York, N.Y.); 27; 3; 3-2021; 291-302
1355-8382
1469-9001
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174742
identifier_str_mv Pascual, Rosa; Segura Morales, Carolina; Omerzu, Manja; Bellora, Nicolás; Belloc, Eulàlia; et al.; mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4; Cold Spring Harbor Laboratory Press; RNA (New York, N.Y.); 27; 3; 3-2021; 291-302
1355-8382
1469-9001
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://rnajournal.cshlp.org/lookup/doi/10.1261/rna.077552.120
info:eu-repo/semantics/altIdentifier/doi/10.1261/rna.077552.120
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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