The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30

Autores
Bercovich, Natalia; Levin, Mariano Jorge; Vazquez, Martin Pablo
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In trypanosomes transcription is polycistronic and individual mRNAs are generated by a trans-splicing/polyadenylation coupled reaction. We identified a divergent trypanosome FIP1-like, a factor required for mRNA 3′ end formation from yeasts to human. Here we showed that it is a nuclear protein with a speckled distribution essential for trypanosome viability. A strong interaction was found between TcFIP1-like and TcCPSF30, a component of the polyadenylation complex. We determined the specific amino acids in each protein involved in the interaction. Significant differences were found between the trypanosome interaction surface and its human counterpart. Although CPSF30/FIP1 interaction is known in other organisms, this is the first report mapping the interaction surface at the amino acid level.
Fil: Bercovich, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Materia
Drug Target
Mrna Processing
Polyadenylation Factors
Protein Interactions
Trypanosome Diseases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79488
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79488
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30Bercovich, NataliaLevin, Mariano JorgeVazquez, Martin PabloDrug TargetMrna ProcessingPolyadenylation FactorsProtein InteractionsTrypanosome Diseaseshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In trypanosomes transcription is polycistronic and individual mRNAs are generated by a trans-splicing/polyadenylation coupled reaction. We identified a divergent trypanosome FIP1-like, a factor required for mRNA 3′ end formation from yeasts to human. Here we showed that it is a nuclear protein with a speckled distribution essential for trypanosome viability. A strong interaction was found between TcFIP1-like and TcCPSF30, a component of the polyadenylation complex. We determined the specific amino acids in each protein involved in the interaction. Significant differences were found between the trypanosome interaction surface and its human counterpart. Although CPSF30/FIP1 interaction is known in other organisms, this is the first report mapping the interaction surface at the amino acid level.Fil: Bercovich, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaAcademic Press Inc Elsevier Science2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79488Bercovich, Natalia; Levin, Mariano Jorge; Vazquez, Martin Pablo; The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 380; 4; 3-2009; 850-8550006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/19338765info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.bbrc.2009.01.182info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:47Zoai:ri.conicet.gov.ar:11336/79488instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:48.237CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
title The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
spellingShingle The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
Bercovich, Natalia
Drug Target
Mrna Processing
Polyadenylation Factors
Protein Interactions
Trypanosome Diseases
title_short The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
title_full The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
title_fullStr The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
title_full_unstemmed The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
title_sort The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
dc.creator.none.fl_str_mv Bercovich, Natalia
Levin, Mariano Jorge
Vazquez, Martin Pablo
author Bercovich, Natalia
author_facet Bercovich, Natalia
Levin, Mariano Jorge
Vazquez, Martin Pablo
author_role author
author2 Levin, Mariano Jorge
Vazquez, Martin Pablo
author2_role author
author
dc.subject.none.fl_str_mv Drug Target
Mrna Processing
Polyadenylation Factors
Protein Interactions
Trypanosome Diseases
topic Drug Target
Mrna Processing
Polyadenylation Factors
Protein Interactions
Trypanosome Diseases
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In trypanosomes transcription is polycistronic and individual mRNAs are generated by a trans-splicing/polyadenylation coupled reaction. We identified a divergent trypanosome FIP1-like, a factor required for mRNA 3′ end formation from yeasts to human. Here we showed that it is a nuclear protein with a speckled distribution essential for trypanosome viability. A strong interaction was found between TcFIP1-like and TcCPSF30, a component of the polyadenylation complex. We determined the specific amino acids in each protein involved in the interaction. Significant differences were found between the trypanosome interaction surface and its human counterpart. Although CPSF30/FIP1 interaction is known in other organisms, this is the first report mapping the interaction surface at the amino acid level.
Fil: Bercovich, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
description In trypanosomes transcription is polycistronic and individual mRNAs are generated by a trans-splicing/polyadenylation coupled reaction. We identified a divergent trypanosome FIP1-like, a factor required for mRNA 3′ end formation from yeasts to human. Here we showed that it is a nuclear protein with a speckled distribution essential for trypanosome viability. A strong interaction was found between TcFIP1-like and TcCPSF30, a component of the polyadenylation complex. We determined the specific amino acids in each protein involved in the interaction. Significant differences were found between the trypanosome interaction surface and its human counterpart. Although CPSF30/FIP1 interaction is known in other organisms, this is the first report mapping the interaction surface at the amino acid level.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79488
Bercovich, Natalia; Levin, Mariano Jorge; Vazquez, Martin Pablo; The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 380; 4; 3-2009; 850-855
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79488
identifier_str_mv Bercovich, Natalia; Levin, Mariano Jorge; Vazquez, Martin Pablo; The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 380; 4; 3-2009; 850-855
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/19338765
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.bbrc.2009.01.182
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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