Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato

Autores
de Godoy, Fabiana; Bermudez, Luisa; Silvestre, Bruno Lira; Souza, Amanda Pereira de; Elbl, Paula; Demarco, Diego; Alseekh, Saleh; Insani, Marina; Buckeridge, Marcos; Almeida, Juliana; Grigioni, Gabriela Maria; Fernie, Alisdair Robert; Carrari, Fernando Oscar; Rossi, Maria Magdalena
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pectin is a main component of cell wall and the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty seven different enzymatic activities have been sugested to be required for pectin biosynthesis but only a few genes have been identified and studied so far. In this work we characterized the tomato galacturonosyltransferase family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbors all orthologous genes to those previously described in A. thaliana and a transcriptional profile revealed that the GAUT4 gene is expressed at higher levels in developing organs. GAUT4 silenced tomato plants exhibited an increment in vegetative biomass associated with the palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition, accumulated less starch along with reduced amount of pectin that coincided with an increase in firmness. Moreover, harvest index was dramatically reduced as a consequence of reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning and allocation. Hence, this cell wall-related gene seems to be key in determining plant growth and fruit production in tomato.
Fil: de Godoy, Fabiana. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Bermudez, Luisa. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Silvestre, Bruno Lira. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Souza, Amanda Pereira de . Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Elbl, Paula. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Demarco, Diego. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Insani, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Buckeridge, Marcos. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Almeida, Juliana. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; Argentina
Fil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossi, Maria Magdalena. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Materia
Tomato
Firmness
Galaturonosyltransferase
Metabolism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3926
Acceder
id CONICETDig_96e07961d52f3f11213511d86adf84e1
oai_identifier_str oai:ri.conicet.gov.ar:11336/3926
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomatode Godoy, FabianaBermudez, LuisaSilvestre, Bruno LiraSouza, Amanda Pereira de Elbl, PaulaDemarco, DiegoAlseekh, SalehInsani, MarinaBuckeridge, MarcosAlmeida, JulianaGrigioni, Gabriela MariaFernie, Alisdair RobertCarrari, Fernando OscarRossi, Maria MagdalenaTomatoFirmnessGalaturonosyltransferaseMetabolismhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pectin is a main component of cell wall and the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty seven different enzymatic activities have been sugested to be required for pectin biosynthesis but only a few genes have been identified and studied so far. In this work we characterized the tomato galacturonosyltransferase family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbors all orthologous genes to those previously described in A. thaliana and a transcriptional profile revealed that the GAUT4 gene is expressed at higher levels in developing organs. GAUT4 silenced tomato plants exhibited an increment in vegetative biomass associated with the palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition, accumulated less starch along with reduced amount of pectin that coincided with an increase in firmness. Moreover, harvest index was dramatically reduced as a consequence of reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning and allocation. Hence, this cell wall-related gene seems to be key in determining plant growth and fruit production in tomato.Fil: de Godoy, Fabiana. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Bermudez, Luisa. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Silvestre, Bruno Lira. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Souza, Amanda Pereira de . Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Elbl, Paula. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Demarco, Diego. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Insani, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Buckeridge, Marcos. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Almeida, Juliana. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; ArgentinaFil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rossi, Maria Magdalena. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; BrasilOxford University Press2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/3926de Godoy, Fabiana; Bermudez, Luisa; Silvestre, Bruno Lira; Souza, Amanda Pereira de ; Elbl, Paula; et al.; Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato; Oxford University Press; Journal of Experimental Botany; 64; 8; 4-2013; 2449-24660022-0957enginfo:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/64/8/2449.longinfo:eu-repo/semantics/altIdentifier/doi/doi:10.1093/jxb/ert106info:eu-repo/semantics/altIdentifier/issn/0022-0957info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654432/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:57Zoai:ri.conicet.gov.ar:11336/3926instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:58.224CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
spellingShingle Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
de Godoy, Fabiana
Tomato
Firmness
Galaturonosyltransferase
Metabolism
title_short Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_full Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_fullStr Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_full_unstemmed Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_sort Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
dc.creator.none.fl_str_mv de Godoy, Fabiana
Bermudez, Luisa
Silvestre, Bruno Lira
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair Robert
Carrari, Fernando Oscar
Rossi, Maria Magdalena
author de Godoy, Fabiana
author_facet de Godoy, Fabiana
Bermudez, Luisa
Silvestre, Bruno Lira
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair Robert
Carrari, Fernando Oscar
Rossi, Maria Magdalena
author_role author
author2 Bermudez, Luisa
Silvestre, Bruno Lira
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair Robert
Carrari, Fernando Oscar
Rossi, Maria Magdalena
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomato
Firmness
Galaturonosyltransferase
Metabolism
topic Tomato
Firmness
Galaturonosyltransferase
Metabolism
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pectin is a main component of cell wall and the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty seven different enzymatic activities have been sugested to be required for pectin biosynthesis but only a few genes have been identified and studied so far. In this work we characterized the tomato galacturonosyltransferase family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbors all orthologous genes to those previously described in A. thaliana and a transcriptional profile revealed that the GAUT4 gene is expressed at higher levels in developing organs. GAUT4 silenced tomato plants exhibited an increment in vegetative biomass associated with the palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition, accumulated less starch along with reduced amount of pectin that coincided with an increase in firmness. Moreover, harvest index was dramatically reduced as a consequence of reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning and allocation. Hence, this cell wall-related gene seems to be key in determining plant growth and fruit production in tomato.
Fil: de Godoy, Fabiana. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Bermudez, Luisa. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Silvestre, Bruno Lira. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Souza, Amanda Pereira de . Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Elbl, Paula. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Demarco, Diego. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Insani, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Buckeridge, Marcos. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Almeida, Juliana. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; Argentina
Fil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossi, Maria Magdalena. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
description Pectin is a main component of cell wall and the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty seven different enzymatic activities have been sugested to be required for pectin biosynthesis but only a few genes have been identified and studied so far. In this work we characterized the tomato galacturonosyltransferase family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbors all orthologous genes to those previously described in A. thaliana and a transcriptional profile revealed that the GAUT4 gene is expressed at higher levels in developing organs. GAUT4 silenced tomato plants exhibited an increment in vegetative biomass associated with the palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition, accumulated less starch along with reduced amount of pectin that coincided with an increase in firmness. Moreover, harvest index was dramatically reduced as a consequence of reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning and allocation. Hence, this cell wall-related gene seems to be key in determining plant growth and fruit production in tomato.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3926
de Godoy, Fabiana; Bermudez, Luisa; Silvestre, Bruno Lira; Souza, Amanda Pereira de ; Elbl, Paula; et al.; Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato; Oxford University Press; Journal of Experimental Botany; 64; 8; 4-2013; 2449-2466
0022-0957
url http://hdl.handle.net/11336/3926
identifier_str_mv de Godoy, Fabiana; Bermudez, Luisa; Silvestre, Bruno Lira; Souza, Amanda Pereira de ; Elbl, Paula; et al.; Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato; Oxford University Press; Journal of Experimental Botany; 64; 8; 4-2013; 2449-2466
0022-0957
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/64/8/2449.long
info:eu-repo/semantics/altIdentifier/doi/doi:10.1093/jxb/ert106
info:eu-repo/semantics/altIdentifier/issn/0022-0957
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654432/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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