Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism

Autores
Bermudez Salazar, Luisa; de Godoy, Fabiana; Baldet, Pierre; Demarco, Diego; Osorio, Sonia; Quadrana, Leandro Daniel; Almeida de Souza, Juliana Beatriz; Asis, Ramón; Gibon, Yves; Fernie, Alisdair R.; Rossi, Maria Magdalena; Carrari, Fernando
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase, sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.
Instituto de Biotecnología
Fil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: de Godoy, Fabiana. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Baldet, Pierre. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia
Fil: Demarco, Diego. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Osorio, Sonia. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almeida de Souza, Juliana Beatriz. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Gibon, Yves. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia
Fil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Fil: Rossi, Maria Magdalena. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plant journal 77 (5) : 676-687. (March 2014)
Materia
Tomate
Solanum Lycopersicum
Azúcar
Metabolismo
Cloroplasto
Tomatoes
Sugar
Metabolism
Chloroplasts
Small Plastidial Protein
Source-sink Partitioning
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4750
Acceder
id INTADig_6f0c443d53602bf50f29fd29bccdfc75
oai_identifier_str oai:localhost:20.500.12123/4750
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolismBermudez Salazar, Luisade Godoy, FabianaBaldet, PierreDemarco, DiegoOsorio, SoniaQuadrana, Leandro DanielAlmeida de Souza, Juliana BeatrizAsis, RamónGibon, YvesFernie, Alisdair R.Rossi, Maria MagdalenaCarrari, FernandoTomateSolanum LycopersicumAzúcarMetabolismoCloroplastoTomatoesSugarMetabolismChloroplastsSmall Plastidial ProteinSource-sink PartitioningLimitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase, sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.Instituto de BiotecnologíaFil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; BrasilFil: de Godoy, Fabiana. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; BrasilFil: Baldet, Pierre. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; FranciaFil: Demarco, Diego. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; BrasilFil: Osorio, Sonia. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Almeida de Souza, Juliana Beatriz. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; BrasilFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Gibon, Yves. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; FranciaFil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; BulgariaFil: Rossi, Maria Magdalena. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; BrasilFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley; Society for Experimental Biology2019-03-26T18:31:28Z2019-03-26T18:31:28Z2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4750https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.124181365-313Xhttps://doi.org/10.1111/tpj.12418Plant journal 77 (5) : 676-687. (March 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:54Zoai:localhost:20.500.12123/4750instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:54.362INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
spellingShingle Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
Bermudez Salazar, Luisa
Tomate
Solanum Lycopersicum
Azúcar
Metabolismo
Cloroplasto
Tomatoes
Sugar
Metabolism
Chloroplasts
Small Plastidial Protein
Source-sink Partitioning
title_short Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_full Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_fullStr Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_full_unstemmed Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_sort Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
dc.creator.none.fl_str_mv Bermudez Salazar, Luisa
de Godoy, Fabiana
Baldet, Pierre
Demarco, Diego
Osorio, Sonia
Quadrana, Leandro Daniel
Almeida de Souza, Juliana Beatriz
Asis, Ramón
Gibon, Yves
Fernie, Alisdair R.
Rossi, Maria Magdalena
Carrari, Fernando
author Bermudez Salazar, Luisa
author_facet Bermudez Salazar, Luisa
de Godoy, Fabiana
Baldet, Pierre
Demarco, Diego
Osorio, Sonia
Quadrana, Leandro Daniel
Almeida de Souza, Juliana Beatriz
Asis, Ramón
Gibon, Yves
Fernie, Alisdair R.
Rossi, Maria Magdalena
Carrari, Fernando
author_role author
author2 de Godoy, Fabiana
Baldet, Pierre
Demarco, Diego
Osorio, Sonia
Quadrana, Leandro Daniel
Almeida de Souza, Juliana Beatriz
Asis, Ramón
Gibon, Yves
Fernie, Alisdair R.
Rossi, Maria Magdalena
Carrari, Fernando
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomate
Solanum Lycopersicum
Azúcar
Metabolismo
Cloroplasto
Tomatoes
Sugar
Metabolism
Chloroplasts
Small Plastidial Protein
Source-sink Partitioning
topic Tomate
Solanum Lycopersicum
Azúcar
Metabolismo
Cloroplasto
Tomatoes
Sugar
Metabolism
Chloroplasts
Small Plastidial Protein
Source-sink Partitioning
dc.description.none.fl_txt_mv Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase, sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.
Instituto de Biotecnología
Fil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: de Godoy, Fabiana. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Baldet, Pierre. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia
Fil: Demarco, Diego. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Osorio, Sonia. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almeida de Souza, Juliana Beatriz. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Gibon, Yves. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia
Fil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Fil: Rossi, Maria Magdalena. Universidade de São Paulo. Departamento de Botânica, Instituto de Biociências; Brasil
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase, sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
2019-03-26T18:31:28Z
2019-03-26T18:31:28Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4750
https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12418
1365-313X
https://doi.org/10.1111/tpj.12418
url http://hdl.handle.net/20.500.12123/4750
https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12418
https://doi.org/10.1111/tpj.12418
identifier_str_mv 1365-313X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley; Society for Experimental Biology
publisher.none.fl_str_mv Wiley; Society for Experimental Biology
dc.source.none.fl_str_mv Plant journal 77 (5) : 676-687. (March 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341364531986432
score 12.623145

Publicaciones similares