Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato

Autores
Godoy, Fabiana de; Bermúdez, Luisa; Lira, Bruno Silvestre; Souza, Amanda Pereira de; Elbl, Paula; Demarco, Diego; Alseekh, Saleh; Insani, Ester Marina; Buckeridge, Marcos; Almeida, Juliana; Grigioni, Gabriela Maria; Fernie, Alisdair R.; Carrari, Fernando; Rossi, Magdalena
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomato
Fil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Bermúdez, Luisa. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Souza, Amanda Pereira de. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Elbl, Paula. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Buckeridge, Marcos. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
Fil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica; Brasil
Fuente
Journal of Experimental Botany 64 (8) : 2449–2466 (May 2013)
Materia
Tomate
Pectinas
Tomatoes
Pectins
Carbon
Carbono
Galacturonosyltransferase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1655
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1655
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spelling Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomatoGodoy, Fabiana deBermúdez, LuisaLira, Bruno SilvestreSouza, Amanda Pereira deElbl, PaulaDemarco, DiegoAlseekh, SalehInsani, Ester MarinaBuckeridge, MarcosAlmeida, JulianaGrigioni, Gabriela MariaFernie, Alisdair R.Carrari, FernandoRossi, MagdalenaTomatePectinasTomatoesPectinsCarbonCarbonoGalacturonosyltransferasePectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomatoFil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Bermúdez, Luisa. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Souza, Amanda Pereira de. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Elbl, Paula. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Buckeridge, Marcos. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica; BrasilFil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; ArgentinaFil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica; Brasil2017-11-02T13:04:18Z2017-11-02T13:04:18Z2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1655https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert1060022-0957 (Print)1460-2431 (Online)https://doi.org/10.1093/jxb/ert106Journal of Experimental Botany 64 (8) : 2449–2466 (May 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:07Zoai:localhost:20.500.12123/1655instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:07.962INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
spellingShingle Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
Godoy, Fabiana de
Tomate
Pectinas
Tomatoes
Pectins
Carbon
Carbono
Galacturonosyltransferase
title_short Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_full Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_fullStr Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_full_unstemmed Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_sort Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
dc.creator.none.fl_str_mv Godoy, Fabiana de
Bermúdez, Luisa
Lira, Bruno Silvestre
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Ester Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author Godoy, Fabiana de
author_facet Godoy, Fabiana de
Bermúdez, Luisa
Lira, Bruno Silvestre
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Ester Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author_role author
author2 Bermúdez, Luisa
Lira, Bruno Silvestre
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Ester Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomate
Pectinas
Tomatoes
Pectins
Carbon
Carbono
Galacturonosyltransferase
topic Tomate
Pectinas
Tomatoes
Pectins
Carbon
Carbono
Galacturonosyltransferase
dc.description.none.fl_txt_mv Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomato
Fil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Bermúdez, Luisa. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Souza, Amanda Pereira de. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Elbl, Paula. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Buckeridge, Marcos. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica; Brasil
Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
Fil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica; Brasil
description Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomato
publishDate 2013
dc.date.none.fl_str_mv 2013-04
2017-11-02T13:04:18Z
2017-11-02T13:04:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1655
https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert106
0022-0957 (Print)
1460-2431 (Online)
https://doi.org/10.1093/jxb/ert106
url http://hdl.handle.net/20.500.12123/1655
https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert106
https://doi.org/10.1093/jxb/ert106
identifier_str_mv 0022-0957 (Print)
1460-2431 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Experimental Botany 64 (8) : 2449–2466 (May 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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