Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii

Autores
Steinhauser, Marie Caroline; Steinhauser, Dirk; Koehl, Karin; Carrari, Fernando Oscar; Gibon, Yves; Fernie, Alisdair R.; Stitt, Mark
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enzymes interact to generate metabolic networks. The activities of more than 22 enzymes from central metabolism were profiled during the development of fruit of the modern tomato cultivar Solanum lycopersicum 'M82' and its wild relative Solanum pennellii (LA0716). In S. pennellii, the mature fruit remains green and contains lower sugar and higher organic acid levels. These genotypes are the parents of a widely used near introgression line population. Enzymes were also profiled in a second cultivar, S. lycopersicum 'Moneymaker', for which data sets for the developmental changes of metabolites and transcripts are available. Whereas most enzyme activities declined during fruit development in the modern S. lycopersicum cultivars, they remained high or even increased in S. pennellii, especially enzymes required for organic acid synthesis. The enzyme profiles were sufficiently characteristic to allow stages of development and cultivars and the wild species to be distinguished by principal component analysis and clustering. Many enzymes showed coordinated changes during fruit development of a given genotype. Comparison of the correlation matrices revealed a large overlap between the two modern cultivars and considerable overlap with S. pennellii, indicating that despite the very different development responses, some basic modules are retained. Comparison of enzyme activity, metabolite profiles, and transcript profiles in S. lycopersicum 'Moneymaker' revealed remarkably little connectivity between the developmental changes of transcripts and enzymes and even less between enzymes and metabolites. We discuss the concept that the metabolite profile is an emergent property that is generated by complex network interactions.
Fil: Steinhauser, Marie Caroline. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Steinhauser, Dirk. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Koehl, Karin. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gibon, Yves. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania
Materia
tomato
fruit metabolism
Solanum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99965
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99965
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennelliiSteinhauser, Marie CarolineSteinhauser, DirkKoehl, KarinCarrari, Fernando OscarGibon, YvesFernie, Alisdair R.Stitt, Marktomatofruit metabolismSolanumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Enzymes interact to generate metabolic networks. The activities of more than 22 enzymes from central metabolism were profiled during the development of fruit of the modern tomato cultivar Solanum lycopersicum 'M82' and its wild relative Solanum pennellii (LA0716). In S. pennellii, the mature fruit remains green and contains lower sugar and higher organic acid levels. These genotypes are the parents of a widely used near introgression line population. Enzymes were also profiled in a second cultivar, S. lycopersicum 'Moneymaker', for which data sets for the developmental changes of metabolites and transcripts are available. Whereas most enzyme activities declined during fruit development in the modern S. lycopersicum cultivars, they remained high or even increased in S. pennellii, especially enzymes required for organic acid synthesis. The enzyme profiles were sufficiently characteristic to allow stages of development and cultivars and the wild species to be distinguished by principal component analysis and clustering. Many enzymes showed coordinated changes during fruit development of a given genotype. Comparison of the correlation matrices revealed a large overlap between the two modern cultivars and considerable overlap with S. pennellii, indicating that despite the very different development responses, some basic modules are retained. Comparison of enzyme activity, metabolite profiles, and transcript profiles in S. lycopersicum 'Moneymaker' revealed remarkably little connectivity between the developmental changes of transcripts and enzymes and even less between enzymes and metabolites. We discuss the concept that the metabolite profile is an emergent property that is generated by complex network interactions.Fil: Steinhauser, Marie Caroline. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Steinhauser, Dirk. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Koehl, Karin. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gibon, Yves. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; AlemaniaAmerican Society of Plant Biologist2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99965Steinhauser, Marie Caroline; Steinhauser, Dirk; Koehl, Karin; Carrari, Fernando Oscar; Gibon, Yves; et al.; Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii; American Society of Plant Biologist; Plant Physiology; 153; 1; 5-2010; 80-980032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/153/1/80info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.110.154336info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862428/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:22Zoai:ri.conicet.gov.ar:11336/99965instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:22.779CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
title Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
spellingShingle Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
Steinhauser, Marie Caroline
tomato
fruit metabolism
Solanum
title_short Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
title_full Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
title_fullStr Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
title_full_unstemmed Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
title_sort Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii
dc.creator.none.fl_str_mv Steinhauser, Marie Caroline
Steinhauser, Dirk
Koehl, Karin
Carrari, Fernando Oscar
Gibon, Yves
Fernie, Alisdair R.
Stitt, Mark
author Steinhauser, Marie Caroline
author_facet Steinhauser, Marie Caroline
Steinhauser, Dirk
Koehl, Karin
Carrari, Fernando Oscar
Gibon, Yves
Fernie, Alisdair R.
Stitt, Mark
author_role author
author2 Steinhauser, Dirk
Koehl, Karin
Carrari, Fernando Oscar
Gibon, Yves
Fernie, Alisdair R.
Stitt, Mark
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv tomato
fruit metabolism
Solanum
topic tomato
fruit metabolism
Solanum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Enzymes interact to generate metabolic networks. The activities of more than 22 enzymes from central metabolism were profiled during the development of fruit of the modern tomato cultivar Solanum lycopersicum 'M82' and its wild relative Solanum pennellii (LA0716). In S. pennellii, the mature fruit remains green and contains lower sugar and higher organic acid levels. These genotypes are the parents of a widely used near introgression line population. Enzymes were also profiled in a second cultivar, S. lycopersicum 'Moneymaker', for which data sets for the developmental changes of metabolites and transcripts are available. Whereas most enzyme activities declined during fruit development in the modern S. lycopersicum cultivars, they remained high or even increased in S. pennellii, especially enzymes required for organic acid synthesis. The enzyme profiles were sufficiently characteristic to allow stages of development and cultivars and the wild species to be distinguished by principal component analysis and clustering. Many enzymes showed coordinated changes during fruit development of a given genotype. Comparison of the correlation matrices revealed a large overlap between the two modern cultivars and considerable overlap with S. pennellii, indicating that despite the very different development responses, some basic modules are retained. Comparison of enzyme activity, metabolite profiles, and transcript profiles in S. lycopersicum 'Moneymaker' revealed remarkably little connectivity between the developmental changes of transcripts and enzymes and even less between enzymes and metabolites. We discuss the concept that the metabolite profile is an emergent property that is generated by complex network interactions.
Fil: Steinhauser, Marie Caroline. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Steinhauser, Dirk. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Koehl, Karin. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gibon, Yves. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania
description Enzymes interact to generate metabolic networks. The activities of more than 22 enzymes from central metabolism were profiled during the development of fruit of the modern tomato cultivar Solanum lycopersicum 'M82' and its wild relative Solanum pennellii (LA0716). In S. pennellii, the mature fruit remains green and contains lower sugar and higher organic acid levels. These genotypes are the parents of a widely used near introgression line population. Enzymes were also profiled in a second cultivar, S. lycopersicum 'Moneymaker', for which data sets for the developmental changes of metabolites and transcripts are available. Whereas most enzyme activities declined during fruit development in the modern S. lycopersicum cultivars, they remained high or even increased in S. pennellii, especially enzymes required for organic acid synthesis. The enzyme profiles were sufficiently characteristic to allow stages of development and cultivars and the wild species to be distinguished by principal component analysis and clustering. Many enzymes showed coordinated changes during fruit development of a given genotype. Comparison of the correlation matrices revealed a large overlap between the two modern cultivars and considerable overlap with S. pennellii, indicating that despite the very different development responses, some basic modules are retained. Comparison of enzyme activity, metabolite profiles, and transcript profiles in S. lycopersicum 'Moneymaker' revealed remarkably little connectivity between the developmental changes of transcripts and enzymes and even less between enzymes and metabolites. We discuss the concept that the metabolite profile is an emergent property that is generated by complex network interactions.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99965
Steinhauser, Marie Caroline; Steinhauser, Dirk; Koehl, Karin; Carrari, Fernando Oscar; Gibon, Yves; et al.; Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii; American Society of Plant Biologist; Plant Physiology; 153; 1; 5-2010; 80-98
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99965
identifier_str_mv Steinhauser, Marie Caroline; Steinhauser, Dirk; Koehl, Karin; Carrari, Fernando Oscar; Gibon, Yves; et al.; Enzyme Activity Profiles during Fruit Development in Tomato Cultivars and Solanum pennellii; American Society of Plant Biologist; Plant Physiology; 153; 1; 5-2010; 80-98
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/153/1/80
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.110.154336
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862428/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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