Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production

Autores
van der Merwe, Margaretha J.; Osorio, Sonia; Araújo, Wagner L.; Balbo, Ilse; Nunes Nesi, Adriano; Maximova, Eugenia; Carrari, Fernando Oscar; Bunik, Victoria I.; Persson, Staffan; Fernie, Alisdair R.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Transgenic tomato (Solanum lycopersicum 'Moneymaker') plants independently expressing fragments of various genes encoding enzymes of the tricarboxylic acid cycle in antisense orientation have previously been characterized as exhibiting altered root growth. In this study, we evaluate the rates of respiration of roots from these lines in addition to determining their total dry weight accumulation. Given that these features were highly correlated, we decided to carry out an evaluation of the cell wall composition in the transformants that revealed a substantial reduction in cellulose. Since the bulk of cellulose is associated with the secondary cell walls in roots, we reasoned that the transformants most likely were deficient in secondary wall cellulose production. Consistent with these findings, cross-sections of the root collar (approximately 15 mm from the junction between root and stem) displayed reduced lignified secondary cell walls for the transformants. In contrast, cell and cell wall patterning displayed no differences in elongating cells close to the root tip. To further characterize the modified cell wall metabolism, we performed feeding experiments in which we incubated excised root tips in [U-14C]glucose in the presence or absence of phosphonate inhibitors of the reaction catalyzed by 2-oxoglutarate dehydrogenase. Taken together, the combined results suggest that restriction of root respiration leads to a deficit in secondary cell wall synthesis. These data are discussed in the context of current models of biomass partitioning and plant growth.
Fil: van der Merwe, Margaretha J.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Osorio, Sonia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Araújo, Wagner L.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Balbo, Ilse. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Nunes Nesi, Adriano. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Maximova, Eugenia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Bunik, Victoria I.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Persson, Staffan. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Fernie, Alisdair R.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Materia
METABOLISM
ROOT GROWTH
TOMATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189132
Acceder
id CONICETDig_4ab0dc28bface696790a0969f2fe6692
oai_identifier_str oai:ri.conicet.gov.ar:11336/189132
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall productionvan der Merwe, Margaretha J.Osorio, SoniaAraújo, Wagner L.Balbo, IlseNunes Nesi, AdrianoMaximova, EugeniaCarrari, Fernando OscarBunik, Victoria I.Persson, StaffanFernie, Alisdair R.METABOLISMROOT GROWTHTOMATOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Transgenic tomato (Solanum lycopersicum 'Moneymaker') plants independently expressing fragments of various genes encoding enzymes of the tricarboxylic acid cycle in antisense orientation have previously been characterized as exhibiting altered root growth. In this study, we evaluate the rates of respiration of roots from these lines in addition to determining their total dry weight accumulation. Given that these features were highly correlated, we decided to carry out an evaluation of the cell wall composition in the transformants that revealed a substantial reduction in cellulose. Since the bulk of cellulose is associated with the secondary cell walls in roots, we reasoned that the transformants most likely were deficient in secondary wall cellulose production. Consistent with these findings, cross-sections of the root collar (approximately 15 mm from the junction between root and stem) displayed reduced lignified secondary cell walls for the transformants. In contrast, cell and cell wall patterning displayed no differences in elongating cells close to the root tip. To further characterize the modified cell wall metabolism, we performed feeding experiments in which we incubated excised root tips in [U-14C]glucose in the presence or absence of phosphonate inhibitors of the reaction catalyzed by 2-oxoglutarate dehydrogenase. Taken together, the combined results suggest that restriction of root respiration leads to a deficit in secondary cell wall synthesis. These data are discussed in the context of current models of biomass partitioning and plant growth.Fil: van der Merwe, Margaretha J.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Osorio, Sonia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Araújo, Wagner L.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Balbo, Ilse. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Nunes Nesi, Adriano. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Maximova, Eugenia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Bunik, Victoria I.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Persson, Staffan. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaFil: Fernie, Alisdair R.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; RusiaAmerican Society of Plant Biologist2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189132van der Merwe, Margaretha J.; Osorio, Sonia; Araújo, Wagner L.; Balbo, Ilse; Nunes Nesi, Adriano; et al.; Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production; American Society of Plant Biologist; Plant Physiology; 153; 2; 6-2010; 611-6210032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/153/2/611/6109561info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.109.149047info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:23Zoai:ri.conicet.gov.ar:11336/189132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:23.35CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
title Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
spellingShingle Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
van der Merwe, Margaretha J.
METABOLISM
ROOT GROWTH
TOMATO
title_short Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
title_full Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
title_fullStr Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
title_full_unstemmed Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
title_sort Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production
dc.creator.none.fl_str_mv van der Merwe, Margaretha J.
Osorio, Sonia
Araújo, Wagner L.
Balbo, Ilse
Nunes Nesi, Adriano
Maximova, Eugenia
Carrari, Fernando Oscar
Bunik, Victoria I.
Persson, Staffan
Fernie, Alisdair R.
author van der Merwe, Margaretha J.
author_facet van der Merwe, Margaretha J.
Osorio, Sonia
Araújo, Wagner L.
Balbo, Ilse
Nunes Nesi, Adriano
Maximova, Eugenia
Carrari, Fernando Oscar
Bunik, Victoria I.
Persson, Staffan
Fernie, Alisdair R.
author_role author
author2 Osorio, Sonia
Araújo, Wagner L.
Balbo, Ilse
Nunes Nesi, Adriano
Maximova, Eugenia
Carrari, Fernando Oscar
Bunik, Victoria I.
Persson, Staffan
Fernie, Alisdair R.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv METABOLISM
ROOT GROWTH
TOMATO
topic METABOLISM
ROOT GROWTH
TOMATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Transgenic tomato (Solanum lycopersicum 'Moneymaker') plants independently expressing fragments of various genes encoding enzymes of the tricarboxylic acid cycle in antisense orientation have previously been characterized as exhibiting altered root growth. In this study, we evaluate the rates of respiration of roots from these lines in addition to determining their total dry weight accumulation. Given that these features were highly correlated, we decided to carry out an evaluation of the cell wall composition in the transformants that revealed a substantial reduction in cellulose. Since the bulk of cellulose is associated with the secondary cell walls in roots, we reasoned that the transformants most likely were deficient in secondary wall cellulose production. Consistent with these findings, cross-sections of the root collar (approximately 15 mm from the junction between root and stem) displayed reduced lignified secondary cell walls for the transformants. In contrast, cell and cell wall patterning displayed no differences in elongating cells close to the root tip. To further characterize the modified cell wall metabolism, we performed feeding experiments in which we incubated excised root tips in [U-14C]glucose in the presence or absence of phosphonate inhibitors of the reaction catalyzed by 2-oxoglutarate dehydrogenase. Taken together, the combined results suggest that restriction of root respiration leads to a deficit in secondary cell wall synthesis. These data are discussed in the context of current models of biomass partitioning and plant growth.
Fil: van der Merwe, Margaretha J.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Osorio, Sonia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Araújo, Wagner L.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Balbo, Ilse. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Nunes Nesi, Adriano. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Maximova, Eugenia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Bunik, Victoria I.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Persson, Staffan. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
Fil: Fernie, Alisdair R.. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania. Moscow State University; Rusia
description Transgenic tomato (Solanum lycopersicum 'Moneymaker') plants independently expressing fragments of various genes encoding enzymes of the tricarboxylic acid cycle in antisense orientation have previously been characterized as exhibiting altered root growth. In this study, we evaluate the rates of respiration of roots from these lines in addition to determining their total dry weight accumulation. Given that these features were highly correlated, we decided to carry out an evaluation of the cell wall composition in the transformants that revealed a substantial reduction in cellulose. Since the bulk of cellulose is associated with the secondary cell walls in roots, we reasoned that the transformants most likely were deficient in secondary wall cellulose production. Consistent with these findings, cross-sections of the root collar (approximately 15 mm from the junction between root and stem) displayed reduced lignified secondary cell walls for the transformants. In contrast, cell and cell wall patterning displayed no differences in elongating cells close to the root tip. To further characterize the modified cell wall metabolism, we performed feeding experiments in which we incubated excised root tips in [U-14C]glucose in the presence or absence of phosphonate inhibitors of the reaction catalyzed by 2-oxoglutarate dehydrogenase. Taken together, the combined results suggest that restriction of root respiration leads to a deficit in secondary cell wall synthesis. These data are discussed in the context of current models of biomass partitioning and plant growth.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189132
van der Merwe, Margaretha J.; Osorio, Sonia; Araújo, Wagner L.; Balbo, Ilse; Nunes Nesi, Adriano; et al.; Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production; American Society of Plant Biologist; Plant Physiology; 153; 2; 6-2010; 611-621
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189132
identifier_str_mv van der Merwe, Margaretha J.; Osorio, Sonia; Araújo, Wagner L.; Balbo, Ilse; Nunes Nesi, Adriano; et al.; Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production; American Society of Plant Biologist; Plant Physiology; 153; 2; 6-2010; 611-621
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/153/2/611/6109561
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.109.149047
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269282891726848
score 13.13397

Publicaciones similares