Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism

Autores
Bermudez Salazar, Luisa Fernanda; de Godoy, Fabiana; Baldet, Pierre; Demarco, Diego; Osorio, Sonia; Quadrana, Leandro Daniel; Almeida de Souza, Juliana Beatriz; Asis, Ramón; Gibon, Yves; Fernie, Alisdair; Rossi, Maria Magdalena; Carrari, Fernando Oscar
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Limitations in our understanding about the mechanisms underlying source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates are increased while the activity of phosphoglucomutase, sugar kinases and invertases are reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggest that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.
Fil: Bermudez Salazar, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Universidade de Sao Paulo; Brasil
Fil: de Godoy, Fabiana. Universidade de Sao Paulo; Brasil
Fil: Baldet, Pierre. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Demarco, Diego. Universidade de Sao Paulo; Brasil
Fil: Osorio, Sonia. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almeida de Souza, Juliana Beatriz. Universidade de Sao Paulo; Brasil
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Gibon, Yves. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Fernie, Alisdair. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Rossi, Maria Magdalena. Universidade de Sao Paulo; Brasil
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Tomato
Small Plastidial Protein
Fruit Metabolism
Source-Sink Partitioning
Chloroplast Metabolism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31869
Acceder
id CONICETDig_cf8173d61d9ea9e08ca47ba8dc85ad14
oai_identifier_str oai:ri.conicet.gov.ar:11336/31869
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolismBermudez Salazar, Luisa Fernandade Godoy, FabianaBaldet, PierreDemarco, DiegoOsorio, SoniaQuadrana, Leandro DanielAlmeida de Souza, Juliana BeatrizAsis, RamónGibon, YvesFernie, AlisdairRossi, Maria MagdalenaCarrari, Fernando OscarTomatoSmall Plastidial ProteinFruit MetabolismSource-Sink PartitioningChloroplast Metabolismhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Limitations in our understanding about the mechanisms underlying source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates are increased while the activity of phosphoglucomutase, sugar kinases and invertases are reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggest that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.Fil: Bermudez Salazar, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Universidade de Sao Paulo; BrasilFil: de Godoy, Fabiana. Universidade de Sao Paulo; BrasilFil: Baldet, Pierre. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique; FranciaFil: Demarco, Diego. Universidade de Sao Paulo; BrasilFil: Osorio, Sonia. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Almeida de Souza, Juliana Beatriz. Universidade de Sao Paulo; BrasilFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Gibon, Yves. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique; FranciaFil: Fernie, Alisdair. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Rossi, Maria Magdalena. Universidade de Sao Paulo; BrasilFil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31869Carrari, Fernando Oscar; Rossi, Maria Magdalena; Fernie, Alisdair; Gibon, Yves; Asis, Ramón; Almeida de Souza, Juliana Beatriz; et al.; Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism; Wiley; Plant Journal; 77; 5; 2-2014; 676-6870960-74121365-313XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tpj.12418/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.12418info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:29Zoai:ri.conicet.gov.ar:11336/31869instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:29.779CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
spellingShingle Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
Bermudez Salazar, Luisa Fernanda
Tomato
Small Plastidial Protein
Fruit Metabolism
Source-Sink Partitioning
Chloroplast Metabolism
title_short Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_full Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_fullStr Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_full_unstemmed Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
title_sort Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
dc.creator.none.fl_str_mv Bermudez Salazar, Luisa Fernanda
de Godoy, Fabiana
Baldet, Pierre
Demarco, Diego
Osorio, Sonia
Quadrana, Leandro Daniel
Almeida de Souza, Juliana Beatriz
Asis, Ramón
Gibon, Yves
Fernie, Alisdair
Rossi, Maria Magdalena
Carrari, Fernando Oscar
author Bermudez Salazar, Luisa Fernanda
author_facet Bermudez Salazar, Luisa Fernanda
de Godoy, Fabiana
Baldet, Pierre
Demarco, Diego
Osorio, Sonia
Quadrana, Leandro Daniel
Almeida de Souza, Juliana Beatriz
Asis, Ramón
Gibon, Yves
Fernie, Alisdair
Rossi, Maria Magdalena
Carrari, Fernando Oscar
author_role author
author2 de Godoy, Fabiana
Baldet, Pierre
Demarco, Diego
Osorio, Sonia
Quadrana, Leandro Daniel
Almeida de Souza, Juliana Beatriz
Asis, Ramón
Gibon, Yves
Fernie, Alisdair
Rossi, Maria Magdalena
Carrari, Fernando Oscar
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomato
Small Plastidial Protein
Fruit Metabolism
Source-Sink Partitioning
Chloroplast Metabolism
topic Tomato
Small Plastidial Protein
Fruit Metabolism
Source-Sink Partitioning
Chloroplast Metabolism
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Limitations in our understanding about the mechanisms underlying source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates are increased while the activity of phosphoglucomutase, sugar kinases and invertases are reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggest that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.
Fil: Bermudez Salazar, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Universidade de Sao Paulo; Brasil
Fil: de Godoy, Fabiana. Universidade de Sao Paulo; Brasil
Fil: Baldet, Pierre. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Demarco, Diego. Universidade de Sao Paulo; Brasil
Fil: Osorio, Sonia. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almeida de Souza, Juliana Beatriz. Universidade de Sao Paulo; Brasil
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Gibon, Yves. Universite de Bordeaux; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Fernie, Alisdair. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Rossi, Maria Magdalena. Universidade de Sao Paulo; Brasil
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Limitations in our understanding about the mechanisms underlying source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates are increased while the activity of phosphoglucomutase, sugar kinases and invertases are reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggest that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31869
Carrari, Fernando Oscar; Rossi, Maria Magdalena; Fernie, Alisdair; Gibon, Yves; Asis, Ramón; Almeida de Souza, Juliana Beatriz; et al.; Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism; Wiley; Plant Journal; 77; 5; 2-2014; 676-687
0960-7412
1365-313X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31869
identifier_str_mv Carrari, Fernando Oscar; Rossi, Maria Magdalena; Fernie, Alisdair; Gibon, Yves; Asis, Ramón; Almeida de Souza, Juliana Beatriz; et al.; Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism; Wiley; Plant Journal; 77; 5; 2-2014; 676-687
0960-7412
1365-313X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tpj.12418/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.12418
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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