Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient

Autores
Sigaut, Lorena; Pearson, John E.; Colman Lerner, Alejandro Ariel; Ponce Dawson, Silvina Martha
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Within this model, the Bcd diffusion coefficient is critical to set the timescale of gradient formation. This coefficient has been measured using two optical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP one. This discrepancy raises the following questions: which estimate is ?correct?; what is the reason for the disparity; and can the SDD model explain Bcd gradient formation within the experimentally observed times. In this paper, we use a simple biophysical model in which Bcd diffuses and interacts with binding sites to show that both the FRAP and the FCS estimates may be correct and compatible with the observed timescale of gradient formation. The discrepancy arises from the fact that FCS and FRAP report on different effective (concentration dependent) diffusion coefficients, one of which describes the spreading rate of the individual Bcd molecules (the messengers) and the other one that of their concentration (the message). The latter is the one that is more relevant for the gradient establishment and is compatible with its formation within the experimentally observed times.
Fil: Sigaut, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Pearson, John E.. Los Alamos National Laboratory;
Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
development
quantitative microscopy
diffusion
bicoid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18030
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18030
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficientSigaut, LorenaPearson, John E.Colman Lerner, Alejandro ArielPonce Dawson, Silvina Marthadevelopmentquantitative microscopydiffusionbicoidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Within this model, the Bcd diffusion coefficient is critical to set the timescale of gradient formation. This coefficient has been measured using two optical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP one. This discrepancy raises the following questions: which estimate is ?correct?; what is the reason for the disparity; and can the SDD model explain Bcd gradient formation within the experimentally observed times. In this paper, we use a simple biophysical model in which Bcd diffuses and interacts with binding sites to show that both the FRAP and the FCS estimates may be correct and compatible with the observed timescale of gradient formation. The discrepancy arises from the fact that FCS and FRAP report on different effective (concentration dependent) diffusion coefficients, one of which describes the spreading rate of the individual Bcd molecules (the messengers) and the other one that of their concentration (the message). The latter is the one that is more relevant for the gradient establishment and is compatible with its formation within the experimentally observed times.Fil: Sigaut, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Pearson, John E.. Los Alamos National Laboratory;Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaPublic Library Of Science2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18030Sigaut, Lorena; Pearson, John E.; Colman Lerner, Alejandro Ariel; Ponce Dawson, Silvina Martha; Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient; Public Library Of Science; Plos Computational Biology; 10; 6; 4-2014; 1-15; e10036291553-734Xenginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1003629info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pcbi.1003629info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:46Zoai:ri.conicet.gov.ar:11336/18030instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:46.887CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
title Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
spellingShingle Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
Sigaut, Lorena
development
quantitative microscopy
diffusion
bicoid
title_short Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
title_full Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
title_fullStr Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
title_full_unstemmed Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
title_sort Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient
dc.creator.none.fl_str_mv Sigaut, Lorena
Pearson, John E.
Colman Lerner, Alejandro Ariel
Ponce Dawson, Silvina Martha
author Sigaut, Lorena
author_facet Sigaut, Lorena
Pearson, John E.
Colman Lerner, Alejandro Ariel
Ponce Dawson, Silvina Martha
author_role author
author2 Pearson, John E.
Colman Lerner, Alejandro Ariel
Ponce Dawson, Silvina Martha
author2_role author
author
author
dc.subject.none.fl_str_mv development
quantitative microscopy
diffusion
bicoid
topic development
quantitative microscopy
diffusion
bicoid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Within this model, the Bcd diffusion coefficient is critical to set the timescale of gradient formation. This coefficient has been measured using two optical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP one. This discrepancy raises the following questions: which estimate is ?correct?; what is the reason for the disparity; and can the SDD model explain Bcd gradient formation within the experimentally observed times. In this paper, we use a simple biophysical model in which Bcd diffuses and interacts with binding sites to show that both the FRAP and the FCS estimates may be correct and compatible with the observed timescale of gradient formation. The discrepancy arises from the fact that FCS and FRAP report on different effective (concentration dependent) diffusion coefficients, one of which describes the spreading rate of the individual Bcd molecules (the messengers) and the other one that of their concentration (the message). The latter is the one that is more relevant for the gradient establishment and is compatible with its formation within the experimentally observed times.
Fil: Sigaut, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Pearson, John E.. Los Alamos National Laboratory;
Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Within this model, the Bcd diffusion coefficient is critical to set the timescale of gradient formation. This coefficient has been measured using two optical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP one. This discrepancy raises the following questions: which estimate is ?correct?; what is the reason for the disparity; and can the SDD model explain Bcd gradient formation within the experimentally observed times. In this paper, we use a simple biophysical model in which Bcd diffuses and interacts with binding sites to show that both the FRAP and the FCS estimates may be correct and compatible with the observed timescale of gradient formation. The discrepancy arises from the fact that FCS and FRAP report on different effective (concentration dependent) diffusion coefficients, one of which describes the spreading rate of the individual Bcd molecules (the messengers) and the other one that of their concentration (the message). The latter is the one that is more relevant for the gradient establishment and is compatible with its formation within the experimentally observed times.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18030
Sigaut, Lorena; Pearson, John E.; Colman Lerner, Alejandro Ariel; Ponce Dawson, Silvina Martha; Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient; Public Library Of Science; Plos Computational Biology; 10; 6; 4-2014; 1-15; e1003629
1553-734X
url http://hdl.handle.net/11336/18030
identifier_str_mv Sigaut, Lorena; Pearson, John E.; Colman Lerner, Alejandro Ariel; Ponce Dawson, Silvina Martha; Messages Do Diffuse Faster Than Messengers: Reconciling disparate estimates of the morphogen Bicoid diffusion coefficient; Public Library Of Science; Plos Computational Biology; 10; 6; 4-2014; 1-15; e1003629
1553-734X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1003629
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pcbi.1003629
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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