Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy

Autores
Virgili Alemán, Inés María; Viñarta, Silvana Carolina; Castellanos, Lucia Ines; Fariña, Julia Ines
Año de publicación
2011
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Scleroglucans from Sclerotium rolsfii ATCC 201126 produced at fermenter scale and ethanol recovered at different times (EPS I: 48 h and EPS II: 72 h) did not exhibit significant variations in MW, degree of polymerization or branching, and adopted a triple helical semirigid structure in aqueous neutral solution. However, they showed certain differences in rheological behaviour, anti-syneresis, emulsifier and suspending properties, hydrogel microstructure and their ability to activate LAL and Glucatell coagulation tests. That may be related to different conformational features such as the triplex expansion degree, which tried to be assessed by fluorescence resonance energy transfer (FRET) spectroscopy. Alkali denaturation of EPSs and subsequent renaturation events over time were evaluated by FRET. EPSs double-labelled with a donor/acceptor fluorophores pair (AP/FITC) were treated with increasing NaOH concentrations (0.015-1.00 M) and a gradual partial opening of the triple-helix rather than a complete strands separation was observed. An isopropanol-processed scleroglucan, EPSi (recovered at 72 h), and a commercial scleroglucan LSCL, also exhibited a similar behaviour. FRET results suggested a decreasing triplex compaction degree as follows: LSCL > EPS I > EPS II > EPSi. Renaturalization showed that even without neutralization the triple-helix conformation could be restored after 96 h.
Fil: Virgili Alemán, Inés María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
CONFORMATION
COMPACTION
SCLEROGLUCAN
FRET
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213131
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/213131
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopyVirgili Alemán, Inés MaríaViñarta, Silvana CarolinaCastellanos, Lucia InesFariña, Julia InesCONFORMATIONCOMPACTIONSCLEROGLUCANFREThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Scleroglucans from Sclerotium rolsfii ATCC 201126 produced at fermenter scale and ethanol recovered at different times (EPS I: 48 h and EPS II: 72 h) did not exhibit significant variations in MW, degree of polymerization or branching, and adopted a triple helical semirigid structure in aqueous neutral solution. However, they showed certain differences in rheological behaviour, anti-syneresis, emulsifier and suspending properties, hydrogel microstructure and their ability to activate LAL and Glucatell coagulation tests. That may be related to different conformational features such as the triplex expansion degree, which tried to be assessed by fluorescence resonance energy transfer (FRET) spectroscopy. Alkali denaturation of EPSs and subsequent renaturation events over time were evaluated by FRET. EPSs double-labelled with a donor/acceptor fluorophores pair (AP/FITC) were treated with increasing NaOH concentrations (0.015-1.00 M) and a gradual partial opening of the triple-helix rather than a complete strands separation was observed. An isopropanol-processed scleroglucan, EPSi (recovered at 72 h), and a commercial scleroglucan LSCL, also exhibited a similar behaviour. FRET results suggested a decreasing triplex compaction degree as follows: LSCL > EPS I > EPS II > EPSi. Renaturalization showed that even without neutralization the triple-helix conformation could be restored after 96 h.Fil: Virgili Alemán, Inés María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPotrero de los FunesArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213131Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-930327-9545CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:47:18Zoai:ri.conicet.gov.ar:11336/213131instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:47:18.517CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
title Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
spellingShingle Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
Virgili Alemán, Inés María
CONFORMATION
COMPACTION
SCLEROGLUCAN
FRET
title_short Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
title_full Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
title_fullStr Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
title_full_unstemmed Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
title_sort Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy
dc.creator.none.fl_str_mv Virgili Alemán, Inés María
Viñarta, Silvana Carolina
Castellanos, Lucia Ines
Fariña, Julia Ines
author Virgili Alemán, Inés María
author_facet Virgili Alemán, Inés María
Viñarta, Silvana Carolina
Castellanos, Lucia Ines
Fariña, Julia Ines
author_role author
author2 Viñarta, Silvana Carolina
Castellanos, Lucia Ines
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv CONFORMATION
COMPACTION
SCLEROGLUCAN
FRET
topic CONFORMATION
COMPACTION
SCLEROGLUCAN
FRET
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Scleroglucans from Sclerotium rolsfii ATCC 201126 produced at fermenter scale and ethanol recovered at different times (EPS I: 48 h and EPS II: 72 h) did not exhibit significant variations in MW, degree of polymerization or branching, and adopted a triple helical semirigid structure in aqueous neutral solution. However, they showed certain differences in rheological behaviour, anti-syneresis, emulsifier and suspending properties, hydrogel microstructure and their ability to activate LAL and Glucatell coagulation tests. That may be related to different conformational features such as the triplex expansion degree, which tried to be assessed by fluorescence resonance energy transfer (FRET) spectroscopy. Alkali denaturation of EPSs and subsequent renaturation events over time were evaluated by FRET. EPSs double-labelled with a donor/acceptor fluorophores pair (AP/FITC) were treated with increasing NaOH concentrations (0.015-1.00 M) and a gradual partial opening of the triple-helix rather than a complete strands separation was observed. An isopropanol-processed scleroglucan, EPSi (recovered at 72 h), and a commercial scleroglucan LSCL, also exhibited a similar behaviour. FRET results suggested a decreasing triplex compaction degree as follows: LSCL > EPS I > EPS II > EPSi. Renaturalization showed that even without neutralization the triple-helix conformation could be restored after 96 h.
Fil: Virgili Alemán, Inés María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Scleroglucans from Sclerotium rolsfii ATCC 201126 produced at fermenter scale and ethanol recovered at different times (EPS I: 48 h and EPS II: 72 h) did not exhibit significant variations in MW, degree of polymerization or branching, and adopted a triple helical semirigid structure in aqueous neutral solution. However, they showed certain differences in rheological behaviour, anti-syneresis, emulsifier and suspending properties, hydrogel microstructure and their ability to activate LAL and Glucatell coagulation tests. That may be related to different conformational features such as the triplex expansion degree, which tried to be assessed by fluorescence resonance energy transfer (FRET) spectroscopy. Alkali denaturation of EPSs and subsequent renaturation events over time were evaluated by FRET. EPSs double-labelled with a donor/acceptor fluorophores pair (AP/FITC) were treated with increasing NaOH concentrations (0.015-1.00 M) and a gradual partial opening of the triple-helix rather than a complete strands separation was observed. An isopropanol-processed scleroglucan, EPSi (recovered at 72 h), and a commercial scleroglucan LSCL, also exhibited a similar behaviour. FRET results suggested a decreasing triplex compaction degree as follows: LSCL > EPS I > EPS II > EPSi. Renaturalization showed that even without neutralization the triple-helix conformation could be restored after 96 h.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213131
Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-93
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213131
identifier_str_mv Conformation and structural compaction of scleroglucan biopolymers as witnessed by FRET spectroscopy; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-93
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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