Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation

Autores
Viñarta, Silvana Carolina; Castellanos, Lucia Ines; Fariña, Julia Ines
Año de publicación
2011
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Scleroglucan is a neutral water-soluble β-1,3-β-1,6-glucan produced by Sclerotium rolfsii ATCC 201126 whose triple helical conformation can be turned into single coiled chains at high pH or temperature and/or in DMSO. Effects of thermal (65, 95 and 150ºC), alkaline (0·01-0·2 N NaOH) and ultrasonic (1, 5 and 10 min with 20% amplitude) treatments onto the solution properties of S. rolfsii scleroglucans (EPS I, EPS II and EPSi at 0·2% w/v) were comparatively evaluated vs. commercial scleroglucan (LSCL). The more drastic assayed conditions (150ºC, 0·2 N NaOH and 10-min ultrasonication) significantly modified the rheological behavior of scleroglucan solutions, with an abrupt decline in apparent viscosity and the loss of pseudoplastic behavior. EPS I was identified as the less sensitive polymer to thermal degradation, whilst EPSi and LSCL were particularly susceptible to alkaline treatment but the more stable ones against ultrasonication. Scleroglucan samples were all denatured (triple helix -> single brand transition) at 150°C and 0·2 N NaOH. Additionally, size exclusion chromatographic (SEC) profiles of control and treated polymer samples revealed aggregates, single chains and triple helices distinctive zones in accordance with the applied treatment.
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
SCLEROTIUM ROLFSII
SCLEROGLUCAN
DENATURATION
CONFORMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213134
Acceder
id CONICETDig_aa9195e79cdbbdf8f74a4279bab5b996
oai_identifier_str oai:ri.conicet.gov.ar:11336/213134
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradationViñarta, Silvana CarolinaCastellanos, Lucia InesFariña, Julia InesSCLEROTIUM ROLFSIISCLEROGLUCANDENATURATIONCONFORMATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Scleroglucan is a neutral water-soluble β-1,3-β-1,6-glucan produced by Sclerotium rolfsii ATCC 201126 whose triple helical conformation can be turned into single coiled chains at high pH or temperature and/or in DMSO. Effects of thermal (65, 95 and 150ºC), alkaline (0·01-0·2 N NaOH) and ultrasonic (1, 5 and 10 min with 20% amplitude) treatments onto the solution properties of S. rolfsii scleroglucans (EPS I, EPS II and EPSi at 0·2% w/v) were comparatively evaluated vs. commercial scleroglucan (LSCL). The more drastic assayed conditions (150ºC, 0·2 N NaOH and 10-min ultrasonication) significantly modified the rheological behavior of scleroglucan solutions, with an abrupt decline in apparent viscosity and the loss of pseudoplastic behavior. EPS I was identified as the less sensitive polymer to thermal degradation, whilst EPSi and LSCL were particularly susceptible to alkaline treatment but the more stable ones against ultrasonication. Scleroglucan samples were all denatured (triple helix -> single brand transition) at 150°C and 0·2 N NaOH. Additionally, size exclusion chromatographic (SEC) profiles of control and treated polymer samples revealed aggregates, single chains and triple helices distinctive zones in accordance with the applied treatment.Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPotrero de los FunesArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213134Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-930327-9545CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:18Zoai:ri.conicet.gov.ar:11336/213134instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:19.013CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
title Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
spellingShingle Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
Viñarta, Silvana Carolina
SCLEROTIUM ROLFSII
SCLEROGLUCAN
DENATURATION
CONFORMATION
title_short Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
title_full Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
title_fullStr Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
title_full_unstemmed Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
title_sort Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation
dc.creator.none.fl_str_mv Viñarta, Silvana Carolina
Castellanos, Lucia Ines
Fariña, Julia Ines
author Viñarta, Silvana Carolina
author_facet Viñarta, Silvana Carolina
Castellanos, Lucia Ines
Fariña, Julia Ines
author_role author
author2 Castellanos, Lucia Ines
Fariña, Julia Ines
author2_role author
author
dc.subject.none.fl_str_mv SCLEROTIUM ROLFSII
SCLEROGLUCAN
DENATURATION
CONFORMATION
topic SCLEROTIUM ROLFSII
SCLEROGLUCAN
DENATURATION
CONFORMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Scleroglucan is a neutral water-soluble β-1,3-β-1,6-glucan produced by Sclerotium rolfsii ATCC 201126 whose triple helical conformation can be turned into single coiled chains at high pH or temperature and/or in DMSO. Effects of thermal (65, 95 and 150ºC), alkaline (0·01-0·2 N NaOH) and ultrasonic (1, 5 and 10 min with 20% amplitude) treatments onto the solution properties of S. rolfsii scleroglucans (EPS I, EPS II and EPSi at 0·2% w/v) were comparatively evaluated vs. commercial scleroglucan (LSCL). The more drastic assayed conditions (150ºC, 0·2 N NaOH and 10-min ultrasonication) significantly modified the rheological behavior of scleroglucan solutions, with an abrupt decline in apparent viscosity and the loss of pseudoplastic behavior. EPS I was identified as the less sensitive polymer to thermal degradation, whilst EPSi and LSCL were particularly susceptible to alkaline treatment but the more stable ones against ultrasonication. Scleroglucan samples were all denatured (triple helix -> single brand transition) at 150°C and 0·2 N NaOH. Additionally, size exclusion chromatographic (SEC) profiles of control and treated polymer samples revealed aggregates, single chains and triple helices distinctive zones in accordance with the applied treatment.
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Scleroglucan is a neutral water-soluble β-1,3-β-1,6-glucan produced by Sclerotium rolfsii ATCC 201126 whose triple helical conformation can be turned into single coiled chains at high pH or temperature and/or in DMSO. Effects of thermal (65, 95 and 150ºC), alkaline (0·01-0·2 N NaOH) and ultrasonic (1, 5 and 10 min with 20% amplitude) treatments onto the solution properties of S. rolfsii scleroglucans (EPS I, EPS II and EPSi at 0·2% w/v) were comparatively evaluated vs. commercial scleroglucan (LSCL). The more drastic assayed conditions (150ºC, 0·2 N NaOH and 10-min ultrasonication) significantly modified the rheological behavior of scleroglucan solutions, with an abrupt decline in apparent viscosity and the loss of pseudoplastic behavior. EPS I was identified as the less sensitive polymer to thermal degradation, whilst EPSi and LSCL were particularly susceptible to alkaline treatment but the more stable ones against ultrasonication. Scleroglucan samples were all denatured (triple helix -> single brand transition) at 150°C and 0·2 N NaOH. Additionally, size exclusion chromatographic (SEC) profiles of control and treated polymer samples revealed aggregates, single chains and triple helices distinctive zones in accordance with the applied treatment.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213134
Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-93
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213134
identifier_str_mv Sclerotium rolfsii scleroglucan: effects of thermal, alkaline and ultrasonic degradation; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-93
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269689452953600
score 13.13397

Publicaciones similares