Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior
- Autores
- Viñarta, Silvana Carolina; Delgado, Osvaldo Daniel; Castellanos de Figueroa, Lucia Ines; Fariña, Julia Inés
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aqueous solutions (0.2%, w/v) of scleroglucans from Sclerotium rolfsii ATCC 201126 from different cultivation time or purification protocol (EPS I, EPS II, EPSi) as well as a commercial scleroglucan (LSCL) exhibited different sensitivity against thermal (65, 95 and 150 ◦C), ultrasonic (1, 5 and 10 min; 20% amplitude) or alkaline (0.01-0.2 N NaOH) treatments. Scleroglucan triple helix usually showed signs of denaturation at 150 ◦C or with 0.2 NaOH with a pronounced decrease in apparent viscosity and loss of pseudoplastic behavior. Differences in sensitivity could be noted depending on the scleroglucan sample, which may be likely related to polysaccharide conformational features, and these latter to production and/or downstream processing conditions. Transmission electronmicroscopy showed scleroglucan topologies in accordance with thermal and alkaline denaturation. Size exclusion chromatography of control scleroglucans revealed elution profiles compatible with macromolecular aggregates which tended to diminish or disappear as thermal, alkali or sonication treatments progressed. Scleroglucan granule dissolution process took ∼8-14 s, according to DIC light microscopy, and showed to be facilitated by addition of NaOH.
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos de Figueroa, Lucia Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Inés. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina - Materia
-
Scleroglucan
Thermal Treatments
Alkaline Treatments
Ultrasonic Treatments - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1825
Ver los metadatos del registro completo
id |
CONICETDig_17c5f4b3440f89853bea1928543f7220 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/1825 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behaviorViñarta, Silvana CarolinaDelgado, Osvaldo DanielCastellanos de Figueroa, Lucia InesFariña, Julia InésScleroglucanThermal TreatmentsAlkaline TreatmentsUltrasonic Treatmentshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aqueous solutions (0.2%, w/v) of scleroglucans from Sclerotium rolfsii ATCC 201126 from different cultivation time or purification protocol (EPS I, EPS II, EPSi) as well as a commercial scleroglucan (LSCL) exhibited different sensitivity against thermal (65, 95 and 150 ◦C), ultrasonic (1, 5 and 10 min; 20% amplitude) or alkaline (0.01-0.2 N NaOH) treatments. Scleroglucan triple helix usually showed signs of denaturation at 150 ◦C or with 0.2 NaOH with a pronounced decrease in apparent viscosity and loss of pseudoplastic behavior. Differences in sensitivity could be noted depending on the scleroglucan sample, which may be likely related to polysaccharide conformational features, and these latter to production and/or downstream processing conditions. Transmission electronmicroscopy showed scleroglucan topologies in accordance with thermal and alkaline denaturation. Size exclusion chromatography of control scleroglucans revealed elution profiles compatible with macromolecular aggregates which tended to diminish or disappear as thermal, alkali or sonication treatments progressed. Scleroglucan granule dissolution process took ∼8-14 s, according to DIC light microscopy, and showed to be facilitated by addition of NaOH.Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Delgado, Osvaldo Daniel. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos de Figueroa, Lucia Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Inés. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaElsevier2013-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1825Viñarta, Silvana Carolina; Delgado, Osvaldo Daniel; Castellanos de Figueroa, Lucia Ines; Fariña, Julia Inés; Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior; Elsevier; Carbohydrate Polymers; 94; 1; 15-4-2013; 496-5040144-8617enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861713000805info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2013.01.063info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:47Zoai:ri.conicet.gov.ar:11336/1825instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:47.474CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior |
title |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior |
spellingShingle |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior Viñarta, Silvana Carolina Scleroglucan Thermal Treatments Alkaline Treatments Ultrasonic Treatments |
title_short |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior |
title_full |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior |
title_fullStr |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior |
title_full_unstemmed |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior |
title_sort |
Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior |
dc.creator.none.fl_str_mv |
Viñarta, Silvana Carolina Delgado, Osvaldo Daniel Castellanos de Figueroa, Lucia Ines Fariña, Julia Inés |
author |
Viñarta, Silvana Carolina |
author_facet |
Viñarta, Silvana Carolina Delgado, Osvaldo Daniel Castellanos de Figueroa, Lucia Ines Fariña, Julia Inés |
author_role |
author |
author2 |
Delgado, Osvaldo Daniel Castellanos de Figueroa, Lucia Ines Fariña, Julia Inés |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Scleroglucan Thermal Treatments Alkaline Treatments Ultrasonic Treatments |
topic |
Scleroglucan Thermal Treatments Alkaline Treatments Ultrasonic Treatments |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Aqueous solutions (0.2%, w/v) of scleroglucans from Sclerotium rolfsii ATCC 201126 from different cultivation time or purification protocol (EPS I, EPS II, EPSi) as well as a commercial scleroglucan (LSCL) exhibited different sensitivity against thermal (65, 95 and 150 ◦C), ultrasonic (1, 5 and 10 min; 20% amplitude) or alkaline (0.01-0.2 N NaOH) treatments. Scleroglucan triple helix usually showed signs of denaturation at 150 ◦C or with 0.2 NaOH with a pronounced decrease in apparent viscosity and loss of pseudoplastic behavior. Differences in sensitivity could be noted depending on the scleroglucan sample, which may be likely related to polysaccharide conformational features, and these latter to production and/or downstream processing conditions. Transmission electronmicroscopy showed scleroglucan topologies in accordance with thermal and alkaline denaturation. Size exclusion chromatography of control scleroglucans revealed elution profiles compatible with macromolecular aggregates which tended to diminish or disappear as thermal, alkali or sonication treatments progressed. Scleroglucan granule dissolution process took ∼8-14 s, according to DIC light microscopy, and showed to be facilitated by addition of NaOH. Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Delgado, Osvaldo Daniel. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Castellanos de Figueroa, Lucia Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Fariña, Julia Inés. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina |
description |
Aqueous solutions (0.2%, w/v) of scleroglucans from Sclerotium rolfsii ATCC 201126 from different cultivation time or purification protocol (EPS I, EPS II, EPSi) as well as a commercial scleroglucan (LSCL) exhibited different sensitivity against thermal (65, 95 and 150 ◦C), ultrasonic (1, 5 and 10 min; 20% amplitude) or alkaline (0.01-0.2 N NaOH) treatments. Scleroglucan triple helix usually showed signs of denaturation at 150 ◦C or with 0.2 NaOH with a pronounced decrease in apparent viscosity and loss of pseudoplastic behavior. Differences in sensitivity could be noted depending on the scleroglucan sample, which may be likely related to polysaccharide conformational features, and these latter to production and/or downstream processing conditions. Transmission electronmicroscopy showed scleroglucan topologies in accordance with thermal and alkaline denaturation. Size exclusion chromatography of control scleroglucans revealed elution profiles compatible with macromolecular aggregates which tended to diminish or disappear as thermal, alkali or sonication treatments progressed. Scleroglucan granule dissolution process took ∼8-14 s, according to DIC light microscopy, and showed to be facilitated by addition of NaOH. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-15 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1825 Viñarta, Silvana Carolina; Delgado, Osvaldo Daniel; Castellanos de Figueroa, Lucia Ines; Fariña, Julia Inés; Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior; Elsevier; Carbohydrate Polymers; 94; 1; 15-4-2013; 496-504 0144-8617 |
url |
http://hdl.handle.net/11336/1825 |
identifier_str_mv |
Viñarta, Silvana Carolina; Delgado, Osvaldo Daniel; Castellanos de Figueroa, Lucia Ines; Fariña, Julia Inés; Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior; Elsevier; Carbohydrate Polymers; 94; 1; 15-4-2013; 496-504 0144-8617 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861713000805 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2013.01.063 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613226325606400 |
score |
13.070432 |